Alignment of multiple glial cell populations in 3D nanofiber scaffolds: Toward the development of multicellular implantable scaffolds for repair of neural injury

Weightman, Alan P.; Jenkins, Stuart I.; Pickard, Michael A.; Chari, Divya M.; Yang, Ying

doi:10.1016/j.nano.2013.09.001

Cited by 36 publications

(37 citation statements)

References 10 publications

(12 reference statements)

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“…Further, in a subcutaneous model, none of the substrates induced cellular orientation parallel to the direction of the substrate topography. It is tempting to hypothesise that two-dimensional imprinted substrates are overwhelmed with body fluids and protein adsorption upon implantation, prohibiting favourable cell / material interaction at the substrate-tissue nano-biointerface and that three-dimensional fibrous constructs are more effective for directional neural [77][78][79], tendon [29,35,80], bone [81][82][83] and skin [84][85][86] neotissue formation and promote relatively enhanced cell growth, motility, matrix deposition and neotissue growth through the provision of a true three-dimensional environment.…”

Section: Discussionmentioning

confidence: 99%

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractControlling the cell-substrate interactions at the bio-interface is becoming an inherent element in the design of implantable devices. Modulation of cellular adhesion in vitro, through topographical cues, is a well-documented process that offers control over subsequent cellular functions. However, it is still unclear whether surface topography can be translated into a clinically functional response in vivo at the tissue / device interface. Herein, we demonstrated that anisotropic substrates with a groove depth of ~317 nm and ~1,988 nm promoted human tenocyte alignment parallel to the underlying topography in vitro. However, the rigid poly(lactic-co-glycolic acid) substrates used in this study upregulated the expression of chondrogenic and osteogenic genes, indicating possible tenocyte trans-differentiation. Of significant importance is that none of the topographies assessed (~37 nm, ~317 nm and ~1,988 nm groove depth) induced extracellular matrix orientation parallel to the substrate orientation in a rat patellar tendon model. These data indicate that two-dimensional imprinting technologies are useful tools for in vitro cell phenotype maintenance, rather than for organised neotissue formation in vivo, should multifactorial approaches that consider both surface topography and substrate rigidity be established.

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Section: Discussionmentioning

confidence: 99%

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

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“…Electrospinning is a versatile and mainstream technique for obtaining engineer conduits with micro to nanoscale topography and high porosity similar to natural extra cellular matrix (Lannutti et al, 2007; Xu et al, 2013). Conduits generated by electrospinning have been proved to induce nerve regeneration (Weightman et al, 2014). Conductive materials have also attracted much attention to be considered as one of the most promising NGC biomaterials because of their properties of biocompatibility, conductivity and suitable hydrophobicity for cell adhesion (Ravichandran et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Polymerizing Pyrrole Coated Poly (l-lactic acid-co-ε-caprolactone) (PLCL) Conductive Nanofibrous Conduit Combined with Electric Stimulation for Long-Range Peripheral Nerve Regeneration

Song

Sun

Liu

et al. 2016

Front. Mol. Neurosci.

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Electrospinning and electric stimulation (ES) are both promising methods to support neuron adhesion and guide extension of neurons for nerve regeneration. Concurrently, all studies focus on either electrospinning for conduits material or ES in vitro study to accelerate nerve regeneration; few work on the combined use of these two strategies or ES in vivo study. Therefore, this study aimed to investigate the abilities of direct current ES through electrospinning conductive polymer composites composed of polypyrrole and Poly (l-lactic acid-co-ε-caprolactone) (PPY/PLCL) in peripheral nerve regeneration. PPY/PLCL composite conduits were synthesized by polymerizing pyrrole coated electrospun PLCL scaffolds. Morphologies and chemical compositions were characterized by scanning electron microscope and attenuated total reflection fourier transform infrared (ATR-FTIR) microscope. Rat pheochromocytoma 12 (PC12) cells and dorsal root ganglia (DRG) cells cultured on PPY/PLCL scaffolds were stimulated with 100 mV/cm for 4 h per day. The median neurite length and cell viability were measured in PC-12 cells. The levels of brain-derived neurotrophic factor (BDNF), glial cell derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) were analyzed in DRG cells. In rats, 15 mm gaps of sciatic nerves were bridged using an autograft, non-stimulated PPY/PLCL conduit and PPY/PLCL conduit stimulated with 100 mV potential, respectively. A 100 mV potential direct current ES was applied for 1 h per day at 1, 3, 5 and 7 days post-implantation. The PPY/PLCL conduits with ES showed a similar performance compared with the autograft group, and significantly better than the non-stimulated PPY/PLCL conduit group. These promising results show that the PPY/PLCL conductive conduits’ combined use with ES has great potential for peripheral nerve regeneration.

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“…This indicates that the frequency and the extent of bone-to-substrate contact were unrelated to the topographical features and that osteogenesis onto the various substrates occurred in a random manner. We recognize that this [152][153][154][155] tendon, [156][157][158] bone [30,159,160] and skin [161][162][163]. However, these studies used 3D nano-and micro-fibrous constructs that allowed cell penetration within the 3D fibrous matrix and subsequent cell-guidance and neotissue growth within this contained/restricted architecture/environment.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Anisotropic Nano- To Micro-Topography on in Vitro and in Vivo Osteogenesis

Azeem

English

Kumar

et al. 2015

Nanomedicine (Lond.)

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Alignment of multiple glial cell populations in 3D nanofiber scaffolds: Toward the development of multicellular implantable scaffolds for repair of neural injury

Cited by 36 publications

References 10 publications

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

Polymerizing Pyrrole Coated Poly (l-lactic acid-co-ε-caprolactone) (PLCL) Conductive Nanofibrous Conduit Combined with Electric Stimulation for Long-Range Peripheral Nerve Regeneration

The Influence of Anisotropic Nano- To Micro-Topography on in Vitro and in Vivo Osteogenesis

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