Alignment of Gold Nanoparticle-Decorated DNA Origami Nanotubes: Substrate Prepatterning versus Molecular Combing

Teschome, Bezu; Facsko, Stefan; Gothelf, Kurt V.; Keller, Adrian

doi:10.1021/acs.langmuir.5b02569

Cited by 38 publications

(48 citation statements)

References 40 publications

(79 reference statements)

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“…The gentlest way to do this is by dipping the sample into pure water for a couple of seconds [24]. While this dipping may result in the dislocation and rearrangement of the adsorbed DNA origami nanostructures [52,53], it does not induce major damage of the Rothemund triangles [24].…”

Section: Resultsmentioning

confidence: 99%

Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability

Kielar

Yang

et al. 2019

Molecules

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DNA origami nanostructures are widely employed in various areas of fundamental and applied research. Due to the tremendous success of the DNA origami technique in the academic field, considerable efforts currently aim at the translation of this technology from a laboratory setting to real-world applications, such as nanoelectronics, drug delivery, and biosensing. While many of these real-world applications rely on an intact DNA origami shape, they often also subject the DNA origami nanostructures to rather harsh and potentially damaging environmental and processing conditions. Furthermore, in the context of DNA origami mass production, the long-term storage of DNA origami nanostructures or their pre-assembled components also becomes an issue of high relevance, especially regarding the possible negative effects on DNA origami structural integrity. Thus, we investigated the effect of staple age on the self-assembly and stability of DNA origami nanostructures using atomic force microscopy. Different harsh processing conditions were simulated by applying different sample preparation protocols. Our results show that staple solutions may be stored at −20 °C for several years without impeding DNA origami self-assembly. Depending on DNA origami shape and superstructure, however, staple age may have negative effects on DNA origami stability under harsh treatment conditions. Mass spectrometry analysis of the aged staple mixtures revealed no signs of staple fragmentation. We, therefore, attribute the increased DNA origami sensitivity toward environmental conditions to an accumulation of damaged nucleobases, which undergo weaker base-pairing interactions and thus lead to reduced duplex stability.

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Section: Resultsmentioning

confidence: 99%

Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability

Kielar

Yang

et al. 2019

Molecules

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“…However, the attachment probability increased with the amount of capture strands per binding sites. High-density nanoparticle assembly on DNA origami templates can be achieved by carefully controlling the concentration of MgCI 2 in buffer, the concentration of nanoparticles, and the hybridization time [ 35 , 46 , 47 ]. Metal nanoparticle growth on DNA templates is extensively discussed in Section 2.4 .…”

Section: Dna Metalizationmentioning

confidence: 99%

Review of the Electrical Characterization of Metallic Nanowires on DNA Templates

Bayrak

Jagtap

Erbe

2018

IJMS

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The use of self-assembly techniques may open new possibilities in scaling down electronic circuits to their ultimate limits. Deoxyribonucleic acid (DNA) nanotechnology has already demonstrated that it can provide valuable tools for the creation of nanostructures of arbitrary shape, therefore presenting an ideal platform for the development of nanoelectronic circuits. So far, however, the electronic properties of DNA nanostructures are mostly insulating, thus limiting the use of the nanostructures in electronic circuits. Therefore, methods have been investigated that use the DNA nanostructures as templates for the deposition of electrically conducting materials along the DNA strands. The most simple such structure is given by metallic nanowires formed by deposition of metals along the DNA nanostructures. Here, we review the fabrication and the characterization of the electronic properties of nanowires, which were created using these methods.

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“…These unique features make DNA origami nanostructures appropriate candidates for use as platform to alignment of nanoparticles for nanodevices fabrication. Consequently, a number of studies have shown the precise organization of metallic nanoparticles on/ in various DNA origami shapes [13,14].…”

Section: Introductionmentioning

confidence: 99%

Origami-Induced Alignment of MNP Inside of DNA Nanotubes

Rafati¹,

Zarrabi²,

Gill³

2020

Int J Med Nano Res

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Background: The advent of nanotubes in the nanotechnology world has led to significant advances in a number of biological and materials application, due to their structural properties such as the surface to volume ratio and potential to surface carrying or inside capsulation any materials. Among the various types of nanotubes, DNA nanotubes, due to their unique characteristics, such as precis controllability and programmability in shape/size/length/diameter and its biological origin compared to different types of nanomaterial, can be the suitable candidate for template patterning alignment and precise organization of nanoparticles at surface or into channel. These characteristics can be used in nanoelectronic devise or in the field of diagnostic nanobiosensores. Methods: Here, we report a new construction methodology for encapsulation of magnetic nanoparticles inside DNA nanotubes channel. Constructed-simultaneously encapsulation of magnetic nanoparticles into the large channel of this tubes leads to "pea-pod" particle alignment in nanotube channel. Results: Transmission electron microscopy and atomic force microscopy confirmed the fabrication of DNA nanotubes contained the magnetic nanoparticles inside the channel. Conclusion: These biohybrid nanomaterial would be proposed as the nanoarray platform in nanobiosensing devices.

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Alignment of Gold Nanoparticle-Decorated DNA Origami Nanotubes: Substrate Prepatterning versus Molecular Combing

Cited by 38 publications

References 40 publications

Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability

Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability

Review of the Electrical Characterization of Metallic Nanowires on DNA Templates

Origami-Induced Alignment of MNP Inside of DNA Nanotubes

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