Alignment and bioactive molecule enrichment of bio-composite scaffolds towards peripheral nerve tissue engineering

Kijeńska‐Gawrońska, Ewa; Bolek, Tomasz; Bil, Monika; Święszkowski, Wojciech

doi:10.1039/c9tb00367c

Cited by 27 publications

(14 citation statements)

References 41 publications

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“…To overcome these limitations, this synthetic polymer is mixed with natural bio-active agents such as proteins, growth factors, etc. A collagen (COL) compound was chosen for the fabrication of our conduit after a prior optimization study, which revealed superior proneurogenic feature of P(LLA-CL) with collagen compared to pristine synthetic polymer [ 14 ]. Polyanilin (PANI) was added because of its electroconductive features, which promote neuroregeneration [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Dębski

Kijeńska‐Gawrońska

Zołocińska

et al. 2021

IJMS

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The aim was to examine the efficiency of a scaffold made of poly (L-lactic acid)-co-poly(ϵ-caprolactone), collagen (COL), polyaniline (PANI), and enriched with adipose-derived stem cells (ASCs) as a nerve conduit in a rat model. P(LLA-CL)-COL-PANI scaffold was optimized and electrospun into a tubular-shaped structure. Adipose tissue from 10 Lewis rats was harvested for ASCs culture. A total of 28 inbred male Lewis rats underwent sciatic nerve transection and excision of a 10 mm nerve trunk fragment. In Group A, the nerve gap remained untouched; in Group B, an excised trunk was used as an autograft; in Group C, nerve stumps were secured with P(LLA-CL)-COL-PANI conduit; in Group D, P(LLA-CL)-COL-PANI conduit was enriched with ASCs. After 6 months of observation, rats were sacrificed. Gastrocnemius muscles and sciatic nerves were harvested for weight, histology analysis, and nerve fiber count analyses. Group A showed advanced atrophy of the muscle, and each intervention (B, C, D) prevented muscle mass decrease (p < 0.0001); however, ASCs addition decreased efficiency vs. autograft (p < 0.05). Nerve fiber count revealed a superior effect in the nerve fiber density observed in the groups with the use of conduit (D vs. B p < 0.0001, C vs. B p < 0.001). P(LLA-CL)-COL-PANI conduits with ASCs showed promising results in managing nerve gap by decreasing muscle atrophy.

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Section: Introductionmentioning

confidence: 99%

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Dębski

Kijeńska‐Gawrońska

Zołocińska

et al. 2021

IJMS

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“…Studies have reported that electrospun nanofibers can reproduce the tissue ECM and offer a sufficient biomimetic environment for cell adhesion, growth, and differentiation, favoring a better tissue regeneration. [ 158,162–164 ] Electrospinning parameters such as polymer concentration to be dissolved in the solvent solution, flow rate, voltage, working distance or ambience conditions can be manipulated to control fiber diameter, porosity, and mechanical strength of the electrospun scaffold. It has been demonstrated that nanofibrous constructs have greater mechanical properties than bulk polymer material and enhance cell proliferation, metabolic activity, and matrix expression.…”

Section: Fiber‐based Engineered Scaffolds For Tendons and Ligamentsmentioning

confidence: 99%

Fibrous Systems as Potential Solutions for Tendon and Ligament Repair, Healing, and Regeneration

Rinoldi

Kijeńska‐Gawrońska

Khademhosseini

et al. 2021

Adv Healthcare Materials

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Tendon and ligament injuries caused by trauma and degenerative diseases are frequent and affect diverse groups of the population. Such injuries reduce musculoskeletal performance, limit joint mobility, and lower people's comfort. Currently, various treatment strategies and surgical procedures are used to heal, repair, and restore the native tissue function. However, these strategies are inadequate and, in some cases, fail to re‐establish the lost functionality. Tissue engineering and regenerative medicine approaches aim to overcome these disadvantages by stimulating the regeneration and formation of neotissues. Design and fabrication of artificial scaffolds with tailored mechanical properties are crucial for restoring the mechanical function of tendons. In this review, the tendon and ligament structure, their physiology, and performance are presented. On the other hand, the requirements are focused for the development of an effective reconstruction device. The most common fiber‐based scaffolding systems are also described for tendon and ligament tissue regeneration like strand fibers, woven, knitted, braided, and braid‐twisted fibrous structures, as well as electrospun and wet‐spun constructs, discussing critically the advantages and limitations of their utilization. Finally, the potential of multilayered systems as the most effective candidates for tendon and ligaments tissue engineering is pointed out.

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“…Such SGN regrowth will also be necessary to reinnervate regenerated hair cells, as that becomes feasible. Strategies to direct neurite guidance have commonly relied on the use of patterned bioactive molecules (Branch et al, 1998;Branch, Wheeler et al, 2001;Gustavsson et al, 2007;Kijenska-Gawronska et al, 2019;Millet et al;Tuft et al, 2014). Using techniques such as microcontact printing, surface modification for selective adsorption, and microfluidics, patterns of peptides that guide neurite growth have been created and used as platforms for directed neurite regeneration (Belisle et al, 2008;Fricke et al, 2011;Honegger et al, 2016;Joo et al, 2015;Klein et al;Wittig et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Interaction of micropatterned topographical and biochemical cues to direct neurite growth from spiral ganglion neurons

Truong

Leigh

Vecchi

et al. 2021

Preprint

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Functional outcomes with neural prosthetic devices, such as cochlear implants, are limited in part due to physical separation between the stimulating elements and the neurons they stimulate. One strategy to close this gap aims to precisely guide neurite regeneration to position the neurites in closer proximity to electrode arrays. Here, we explore the ability of micropatterned biochemical and topographic guidance cues, singly and in combination, to direct the growth of spiral ganglion neuron (SGN) neurites, the neurons targeted by cochlear implants. Photopolymerization of methacrylate monomers was used to form unidirectional topographical features of ridges and grooves in addition to multidirectional patterns with 90o angle turns. Microcontact printing was also used to create similar uni- and multi-directional patterns of peptides on polymer surfaces. Biochemical cues included peptides that facilitate (laminin, LN) or repel (EphA4-Fc) neurite growth. On flat surfaces, SGN neurites preferentially grew on LN-coated stripes and avoided EphA4-Fc-coated stripes. LN or EphA4-Fc was selectively adsorbed onto the ridges or grooves to test the neurite response to a combination of topographical and biochemical cues. Coating the ridges with EphA4-Fc and grooves with LN lead to enhanced SGN alignment to topographical patterns. Conversely, EphA4-Fc coating on the grooves or LN coating on the ridges tended to disrupt alignment to topographical patterns. SGN neurites respond to combinations of topographical and biochemical cues and surface patterning that leverages both cues enhance guided neurite growth.

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Alignment and bioactive molecule enrichment of bio-composite scaffolds towards peripheral nerve tissue engineering

Abstract: Providing topographical cues along with chemical and biological factors is essential for biomimetic scaffolds applied in nerve tissue engineering.

Cited by 27 publications

References 41 publications

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Fibrous Systems as Potential Solutions for Tendon and Ligament Repair, Healing, and Regeneration

Interaction of micropatterned topographical and biochemical cues to direct neurite growth from spiral ganglion neurons

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