Aligned PLGA/HA nanofibrous nanocomposite scaffolds for bone tissue engineering

Jose, Moncy V.; Thomas, Vinoy; Johnson, Kalonda T; Dean, Derrick; Nyairo, Elijah

doi:10.1016/j.actbio.2008.07.019

Cited by 361 publications

(251 citation statements)

References 42 publications

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“…Other materials that can be used in this area are polymer/hydroxyapatite (HAp) nanocomposites e.g. chitozan/HAp PLGA/HAp [11,12]. The efficacy of silk nanofibers in filling bone defects has been also proven [13].…”

Section: Regeneration Of Bone Tissuementioning

confidence: 99%

Potential Applications of Nonwoven Polymer Mats – Review

Louda

Fijalkowski

Rożek

et al. 2013

Contemporary Materials

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Nonwoven polymer mats with fibre diameters in the nanometer and micrometer range are distinguished for their high porosity with very small pore size, interconnectivity and controllable mesh thickness. These characteristics associated to the easy way to obtain the polymer nanofibres and a large surface area to volume ratio make the nonwoven fiber mats a suitable material for different applications, such as biosensor, electronic materials and filters. Polymer nanofibers mats are being considered for use in biomedical applications, including the production of artificial blood vessels, scaffolds for engineered tissues, wound dressings.

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Section: Regeneration Of Bone Tissuementioning

confidence: 99%

Potential Applications of Nonwoven Polymer Mats – Review

Louda

Fijalkowski

Rożek

et al. 2013

Contemporary Materials

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“…The levels of ions, glucose, proteins, enzymes, hormones or metabolites including glutamic-pyruvic transaminase, urea, total bilirubin, cholesterol, triglyceride, creatine kinase, and alkaline phosphatase were measured in the blood (Jose et al, 2009;Niu et al, 2009). …”

Section: Biochemical Blood Indicesmentioning

confidence: 99%

Biocompatibility of differently proportioned HA/PLGA/BMP-2 composite biomaterials in rabbits

Guo

Han

et al. 2015

Genet. Mol. Res.

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“…2 Nanofibrous scaffolds have been prepared mainly from natural or synthetic polymers, such as collagen and elastin, 3 silk fibroin, 4 chitosan, 5 peptides with ligands for cell adhesion receptors, 6 polyurethane, 7 polycaprolactone, 8 polylactide 9 and particularly its copolymers with polyglycolide. [10][11][12][13][14][15] In our earlier studies, and also in studies by other authors, poly (lactide-co-glycolide) (PLGA) has proved to be an appropriate material for the construction of porous and fibrous scaffolds for bone tissue engineering. 16 In comparison with pure polylactic acid (PLA), the PLGA copolymer is less brittle, and in comparison with pure polyglycolide is less degradable (ie, less prone to hydrolytic degradation).…”

Section: Introductionmentioning

confidence: 99%

Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering

Pařízek

Douglas²,

Novotná³

et al. 2012

IJN

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Background: Nanofibrous scaffolds loaded with bioactive nanoparticles are promising materials for bone tissue engineering. Methods: In this study, composite nanofibrous membranes containing a copolymer of L-lactide and glycolide (PLGA) and diamond nanoparticles were fabricated by an electrospinning technique. PLGA was dissolved in a mixture of methylene chloride and dimethyl formamide (2:3) at a concentration of 2.3 wt%, and nanodiamond (ND) powder was added at a concentration of 0.7 wt% (about 23 wt% in dry PLGA). Results: In the composite scaffolds, the ND particles were either arranged like beads in the central part of the fibers or formed clusters protruding from the fibers. In the PLGA-ND membranes, the fibers were thicker (diameter 270 ± 9 nm) than in pure PLGA meshes (diameter 218 ± 4 nm), but the areas of pores among these fibers were smaller than in pure PLGA samples (0.46 ± 0.02 µm 2 versus 1.28 ± 0.09 µm 2 in pure PLGA samples). The PLGA-ND membranes showed higher mechanical resistance, as demonstrated by rupture tests of load and deflection of rupture probe at failure. Both types of membranes enabled the attachment, spreading, and subsequent proliferation of human osteoblast-like MG-63 cells to a similar extent, although these values were usually lower than on polystyrene dishes. Nevertheless, the cells on both types of membranes were polygonal or spindle-like in shape, and were distributed homogeneously on the samples. From days 1-7 after seeding, their number rose continuously, and at the end of the experiment, these cells were able to create a confluent layer. At the same time, the cell viability, evaluated by a LIVE/DEAD viability/cytotoxicity kit, ranged from 92% to 97% on both types of membranes. In addition, on PLGA-ND membranes, the cells formed well developed talin-containing focal adhesion plaques. As estimated by the determination of tumor necrosis factor-alpha levels in the culture medium and concentration of intercellular adhesion molecule-1, MG-63 cells, and RAW 264.7 macrophages on these membranes did not show considerable inflammatory activity. Conclusion:This study shows that nanofibrous PLGA membranes loaded with diamond nanoparticles have interesting potential for use in bone tissue engineering.

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Aligned PLGA/HA nanofibrous nanocomposite scaffolds for bone tissue engineering

Cited by 361 publications

References 42 publications

Potential Applications of Nonwoven Polymer Mats – Review

Potential Applications of Nonwoven Polymer Mats – Review

Biocompatibility of differently proportioned HA/PLGA/BMP-2 composite biomaterials in rabbits

Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering

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