Aligned microcontact printing of micrometer-scale poly-L-Lysine structures for controlled growth of cultured neurons on planar microelectrode arrays

James, Conrad D.; Davis, R.; Meyer, Michael J.; Turner, A. M.P.; Turner, Stephen W.; Withers, Ginger S.; Kam, Lance C.; Banker, Gary; Craighead, Harold G.; Issacson, M.; Turner, James N.; Shain, William

doi:10.1109/10.817614

Cited by 188 publications

(137 citation statements)

References 32 publications

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“…Many cell types have been successfully patterned with microfluidics [14][15][16], lCP [17], inkjet printing [18,19], plasma treatment [20], self-assembled monolayers [21][22][23][24], self-assembled constructs [25], laser scanning lithography [26], atomic force microscope lithography, dip-pen nanolithography [27], topography [28,29], carbon nano-tubes [30], or their combinations [31,32]. Neurons are, however, distinctive cells with highly polarized morphology, much smaller somata, and thus few anchoring points for adhesion in comparison to most types of adherent mammalian cells.…”

Section: Introductionmentioning

confidence: 99%

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

et al. 2012

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By quantitatively comparing a variety of macromolecular surface coating agents, we discovered that surface coating strongly modulates the adhesion and morphogenesis of primary hippocampal neurons and serves as a switch of somata clustering and neurite fasciculation in vitro. The kinetics of neuronal adhesion on poly-lysine-coated surfaces is much faster than that on laminin and Matrigel-coated surfaces, and the distribution of adhesion is more homogenous on poly-lysine. Matrigel and laminin, on the other hand, facilitate neuritogenesis more than poly-lysine does. Eventually, on Matrigel-coated surfaces of self-assembled monolayers, neurons tend to undergo somata clustering and neurite fasciculation. By replacing coating proteins with cerebral astrocytes, and patterning neurons on astrocytes through self-assembled monolayers, microfluidics and micro-contact printing, we found that astrocyte promotes soma adhesion and astrocyte processes guide neurites. There, astrocytes could be a versatile substrate in engineering neuronal networks in vitro. Besides, quantitative measurements of cellular responses on various coatings would be valuable information for the neurobiology community in the choice of the most appropriate coating strategy.

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Section: Introductionmentioning

confidence: 99%

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

et al. 2012

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“…Cell positioning onto electrodes can be improved by surface micropatterning to improve the sensitivity and repeatability of MEA recordings. James et al 178 microstamped poly lysine grids (1-2 μm line width) aligned to underlying MEA of Au electrodes. Rat hippocampal neurons attached and neurites extended faithfully on the poly lysine areas, thus crossing over to the electrode sites.…”

Section: Extracellular Electrode Arrays For Multisite Recording and Smentioning

confidence: 99%

“…Extracellular recordings are noninvasive and allow for long-term recordings for multiple sites, but suffer from very low signal-to-noise ratios because of imprecise positioning of the cell relative to the electrode surface (both in the vertical and horizontal directions). To better define the cell-to-electrode distance, electrodes have been coated with ECM proteins, 177 poly lysine, 178,179 or silane-based monolayers. 180 In addition, electroplating of Pt-back 181 or coating metal electrode with electrically conductive polymers (e.g., polypyrrole) 182 can be used to lower the impedance of the electrode sites.…”

Section: Extracellular Electrode Arrays For Multisite Recording and Smentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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“…The combination of these two approaches will give both spatial and temporal control over the formation of neuronal circuits, allowing scientists to tease apart essential elements of how these networks form and determine what kinds of communication take place within them. For example, microfabrication has already been used to align a substrate pattern with an electrode array, increasing the probability that cells will contact a recording electrode [10]. If cells can be placed on to electrodes with precision, then the patterned substrate could be a molecule that promotes a specific aspect of development (e.g.…”

Section: Future Prospects For Printing Cellsmentioning

confidence: 99%

New ways to print living cells promise breakthroughs for engineering complex tissues in vitro

Withers

2006

Biochemical Journal

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The ability to control the placement of cells and the assembly of networks in vitro has tremendous potential for understanding the regulation of development as well as for generating artificial tissues. To date, most engineering tools that can place materials with precision are not compatible with the requirements of living cells, and so approaches to tissue engineering have focused on patterning substrates as a way of controlling cell growth rather than patterning cells directly. In this issue of Biochemical Journal, however, Eagles et al. adapt electrohydrodynamic printing technology to 'print' living cells from a neuronal cell line on to a substrate. The importance of this approach is that it has the potential for unprecedented control over the position of cells in culture by directly placing them, thus allowing for the systematic assembly of cell networks.

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Aligned microcontact printing of micrometer-scale poly-L-Lysine structures for controlled growth of cultured neurons on planar microelectrode arrays

Cited by 188 publications

References 32 publications

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

New ways to print living cells promise breakthroughs for engineering complex tissues in vitro

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