Aligned fibrous PVDF-TrFE scaffolds with Schwann cells support neurite extension and myelination <i>in vitro</i>

Wu, Siliang; Chen, Ming Shuo; Maurel, Patrice; Lee, Young Soon; Bunge, Mary Bartlett; Arinzeh, Treena Livingston

doi:10.1088/1741-2552/aac77f

Cited by 62 publications

(66 citation statements)

References 80 publications

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“…Electrospun fibers can be modified to specifically promote cell proliferation in the context of wound regeneration, [30,31,34] and piezoelectric materials in particular have a unique capacity for enhancing replication of numerous cell types. [16,54,59,67] For instance, Wang et al demonstrated that modulation of the piezoelectric effect in PVDF-TrFE nanofibers with targeted vibrations can increase the proliferation rate of fibroblasts 1.6 fold. [68] Therefore, these results in conjunction with the adhesion assay show both unaligned and aligned scaffolds are biocompatible and capable of facilitating cell proliferation.…”

Section: Cell Adhesion and Integration With Scaffoldmentioning

confidence: 99%

“…It is also possible to subvert this caveat by developing integrated devices, such as 3D electrospun, aligned nerve conduits, that incorporate additional material technology to affect cell phenotypes in feasible clinical applications. [16,41]…”

Section: Cell Adhesion and Integration With Scaffoldmentioning

confidence: 99%

“…Qualitatively, our fibers promoted alignment in both Schwann cells and fibroblasts ( Figure 8A) consistent with previous work. [16,18,68] To further confirm the extent of alignment, we performed fast Fourier transformations (FFT) on F-actin labeled images to examine alignment of the cell cytoskeleton ( Figure 8B,C). Full width at half-maximum values were obtained and used as quantification for cell alignment.…”

Section: Cell Alignment On Scaffoldsmentioning

confidence: 99%

“…In vitro models have been shown to promote osteogenic differentiation of human mesenchymal cells and early mineralization of the corresponding ECM, [12] differentiation of human neural stem/progenitor cells and neuron mimicking PC-12 cells, [13][14][15] neuron and neurite extension of dorsal root ganglia, [16] and alignment and proliferation of Schwann cells and fibroblasts. [16,17] Though scarce in total, in vivo models have utilized PVDF-TrFE as nerve conduits to enhance regeneration of axons, ancillary cells, and neurotrophic factors such as astrocyte processes and blood vessels. [18,19] The tunable physical, chemical, and electrical properties of PVDF-TrFE make fabricated scaffolds an ideal template for PNS biomaterials.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Piezoelectric PVDF‐TrFE Fibrous Scaffold to Guide Cell Adhesion, Proliferation, and Alignment

Orkwis

Wolf

Shahid

et al. 2020

Macromolecular Bioscience

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Severe peripheral nervous system injuries currently hold limited therapeutic solutions. Existing clinical techniques such as autografts, allografts, and newer nerve guidance conduits have shown variable outcomes in functional recovery, adverse immune responses, and in some cases low or minimal availability. This can be attributed in part to the lack of chemical, physical, and electrical cues directing both nerve guidance and regeneration. To address this pressing clinical issue, electrospun nanofibers and microfibers composed of piezoelectric polyvinylidene flouride‐triflouroethylene (PVDF‐TrFE) have been introduced as an alternative template for tissue engineered biomaterials, specifically as it pertains to their relevance in soft tissue and nerve repair. Here, biocompatible scaffolds of PVDF‐TrFE are fabricated and their ability to generate an electrical response to mechanical deformations and produce a suitable regenerative microenvironment is examined. It is determined that 20% (w/v) PVDF‐TrFE in (6:4) dimethyl formamide (DMF):acetone solvent maintains a desirable piezoelectric coefficient and the proper physical and electrical characteristics for tissue regeneration. Further, it is concluded that scaffolds of varying thickness promoted the adhesion and alignment of Schwann cells and fibroblasts. This work offers a prelude to further advancements in nanofibrous technology and a promising outlook for alternative, autologous remedies to peripheral nerve damage.

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Section: Cell Adhesion and Integration With Scaffoldmentioning

confidence: 99%

Section: Cell Adhesion and Integration With Scaffoldmentioning

confidence: 99%

Section: Cell Alignment On Scaffoldsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of a Piezoelectric PVDF‐TrFE Fibrous Scaffold to Guide Cell Adhesion, Proliferation, and Alignment

Orkwis

Wolf

Shahid

et al. 2020

Macromolecular Bioscience

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“…Scaffolds were prepared using the electrospinning technique. Random or aligned fibrous PVDF-TrFE scaffolds were fabricated by modifying previous methods as described 45 A horizontal electrospinning setup was used to fabricate the PCL and PLA scaffolds. Solutions (15% w/v) were prepared by weighing the appropriate amount of PCL or PLA pellets and dissolving by stirring in dichloromethane.…”

Section: Fabrication Of Scaffoldsmentioning

confidence: 99%

Artificial scaffolds that mimic the plant extracellular environment for the culture and attachment of plant cells

Calcutt

Vincent

Dean

et al. 2020

Preprint

Self Cite

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Plant growth and development involves an intricate program of cell division and cell expansionto generate different cell types, tissue patterns and organ shapes. Plant cells are stuck together by their cell walls and the spatial context of cells within tissues plays a critical role in cell fate specification and morphogenesis. An in vitro model system to study plant development and its regulation by various extrinsic and intrinsic factors requires the ability to mimic the physical interactions between cells and their environment. Here, we present a set of artificial scaffolds to which cultured tobacco BY-2 cells adhere without causing morphological abnormalities. These scaffolds mimic native plant cell walls in terms of their fibrous nature, charge, hydrophobicity and piezoelectricity. We found that the extent of plant cell adhesion was essentially insensitive to the stiffness, fiber dimension, and fiber orientation of the scaffolds, but was affected by the piezoelectric properties of scaffolds where adhesion increased on piezoelectric materials. We also found that the plant cell wall polysaccharide, pectin, is largely responsible for adhesion to scaffolds, analogous to pectin-mediated adhesion of plant cells in tissues. Together, this work establishes biomimetic scaffolds that realistically emulate the plant tissue environment and provide the capability to develop microfluidic devices to study how cell-cell and cell-matrix interactions affect plant developmental pathways.

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Electrospinning Piezoelectric Fibers for Biocompatible Devices

Azimi

Milazzo

Lazzeri

et al. 2019

Adv Healthcare Materials

110

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The field of nanotechnology has been gaining great success due to its potential in developing new generations of nanoscale materials with unprecedented properties and enhanced biological responses. This is particularly exciting using nanofibers, as their mechanical and topographic characteristics can approach those found in naturally occurring biological materials. Electrospinning is a key technique to manufacture ultrafine fibers and fiber meshes with multifunctional features, such as piezoelectricity, to be available on a smaller length scale, thus comparable to subcellular scale, which makes their use increasingly appealing for biomedical applications. These include biocompatible fiber-based devices as smart scaffolds, biosensors, energy harvesters and nanogenerators for the human body. This paper provides a comprehensive review of current studies focused on the fabrication of ultrafine polymeric and ceramic piezoelectric fibers specifically designed for, or with the potential to be translated toward, biomedical applications. It provides an applicative and technical overview of the biocompatible piezoelectric fibers, with actual and potential applications, an understanding of the electrospinning process, and the properties of nanostructured fibrous materials, including the available modeling approaches. Ultimately, this This article is protected by copyright. All rights reserved. 3 review aims at enabling a future vision on the impact of these nanomaterials as stimuli-responsive devices in the human body.

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Aligned fibrous PVDF-TrFE scaffolds with Schwann cells support neurite extension and myelination in vitro

Abstract: This study demonstrated PVDF-TrFE scaffolds containing aligned fibers supported SC-neurite extension and myelination. The combination of SCs and PVDF-TrFE scaffolds may be a promising tissue engineering strategy for spinal cord repair.

Cited by 62 publications

References 80 publications

Development of a Piezoelectric PVDF‐TrFE Fibrous Scaffold to Guide Cell Adhesion, Proliferation, and Alignment

Development of a Piezoelectric PVDF‐TrFE Fibrous Scaffold to Guide Cell Adhesion, Proliferation, and Alignment

Artificial scaffolds that mimic the plant extracellular environment for the culture and attachment of plant cells

Electrospinning Piezoelectric Fibers for Biocompatible Devices

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