Aligned carbon nanotube reinforced polymeric scaffolds with electrical cues for neural tissue regeneration

Gupta, Pallavi; Sharan, Shruti; Roy, Partha; Lahiri, Debrupa

doi:10.1016/j.carbon.2015.08.107

Cited by 89 publications

(69 citation statements)

References 35 publications

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“…Graphene, GBNs, and CNTs are being investigated as potential suitable materials for the growth of NSCs, neurons, and glial cells and for their ability to modulate the function of neuronal circuits in vitro (Li et al, 2011, 2013; Park et al, 2011; Lorenzoni et al, 2013; Solanki et al, 2013; Tang et al, 2013; Shah et al, 2014; Gupta et al, 2015; Vicentini et al, 2015; Weaver and Cui, 2015; Guo et al, 2016c; Marchesan et al, 2016). However, in these previous studies cells were plated on those materials previously coated with extracellular matrix proteins, which are known to promote cell adhesion, differentiation, and neurite outgrowth (Vicario et al, 1993; Calof et al, 1994; Specht et al, 2004; Otaegi et al, 2006; Nishimune et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, cells were plated on graphene composites, graphene oxides, or on reduced graphene oxides with different surface charges and degree of electrical, photo, and laser stimulation (Akhavan and Ghaderi, 2013a,b, 2014; Tu et al, 2013a, 2014; Akhavan et al, 2014, 2015; Guo et al, 2016a). Similarly, both uncoated and coated functionalized single-walled CNTs (SWCNTs) and multi-walled CNTs (MWCNTs) as well as aligned CNTs and nanofibers have been reported to permit and stimulate neuronal growth and the formation of active synaptic contacts (Jan and Kotov, 2007; Malarkey et al, 2009; Cellot et al, 2011; Jin et al, 2011; Fabbro et al, 2013; Gupta et al, 2015; Vicentini et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

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In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

Defteralı

Verdejo

Majeed

et al. 2016

Front. Bioeng. Biotechnol.

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Graphene, graphene-based nanomaterials (GBNs), and carbon nanotubes (CNTs) are being investigated as potential substrates for the growth of neural cells. However, in most in vitro studies, the cells were seeded on these materials coated with various proteins implying that the observed effects on the cells could not solely be attributed to the GBN and CNT properties. Here, we studied the biocompatibility of uncoated thermally reduced graphene (TRG) and poly(vinylidene fluoride) (PVDF) membranes loaded with multi-walled CNTs (MWCNTs) using neural stem cells isolated from the adult mouse olfactory bulb (termed aOBSCs). When aOBSCs were induced to differentiate on coverslips treated with TRG or control materials (polyethyleneimine-PEI and polyornithine plus fibronectin-PLO/F) in a serum-free medium, neurons, astrocytes, and oligodendrocytes were generated in all conditions, indicating that TRG permits the multi-lineage differentiation of aOBSCs. However, the total number of cells was reduced on both PEI and TRG. In a serum-containing medium, aOBSC-derived neurons and oligodendrocytes grown on TRG were more numerous than in controls; the neurons developed synaptic boutons and oligodendrocytes were more branched. In contrast, neurons growing on PVDF membranes had reduced neurite branching, and on MWCNTs-loaded membranes oligodendrocytes were lower in numbers than in controls. Overall, these findings indicate that uncoated TRG may be biocompatible with the generation, differentiation, and maturation of aOBSC-derived neurons and glial cells, implying a potential use for TRG to study functional neuronal networks.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

Defteralı

Verdejo

Majeed

et al. 2016

Front. Bioeng. Biotechnol.

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“…Among the different nanofillers used as reinforcement phase, the most used in the development of CHI nanocomposites are: (i) layered silicates, such as clay [27–29]; (ii) metal/ceramic nanoparticles [30–32]; (iii) carbon nanotubes [33–35]; and more recently (iv) graphene based materials. [36–38] In the next subsections, each of these fillers will be analyzed.…”

Section: Chitosan Nanocompositesmentioning

confidence: 99%

Chitosan nanocomposites based on distinct inorganic fillers for biomedical applications

Moura

Mano

Paiva

et al. 2016

Science and Technology of Advanced Materials

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Chitosan (CHI), a biocompatible and biodegradable polysaccharide with the ability to provide a non-protein matrix for tissue growth, is considered to be an ideal material in the biomedical field. However, the lack of good mechanical properties limits its applications. In order to overcome this drawback, CHI has been combined with different polymers and fillers, leading to a variety of chitosan-based nanocomposites. The extensive research on CHI nanocomposites as well as their main biomedical applications are reviewed in this paper. An overview of the different fillers and assembly techniques available to produce CHI nanocomposites is presented. Finally, the properties of such nanocomposites are discussed with particular focus on bone regeneration, drug delivery, wound healing and biosensing applications.

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“…The oriented architectures of carbon nanotubes guide cell orientation at an efficient rate, although the biocompatibility of these materials is low which leads to poor cell proliferation . The driving forces for nanotube ordered patterns include electrical field, shear, and magnetic forces . More recently, the anisotropic patterns of nanotubes were made via capillary force induced self‐assembly.…”

Section: Introductionmentioning

confidence: 99%

Clay Nanotubes Aligned with Shear Forces for Mesenchymal Stem Cell Patterning

Zhao

Zhou

Lvov

et al. 2019

Small

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Aligned halloysite nanotubes on solid substrates are fabricated by a shearing method with brush assistance. These clay nanotubes are aligned by shear force in strip‐like patterns accomplished with drying ordering at elevated temperatures. The nanotubes' orientation is governed by “coffee‐ring” formation mechanisms depending on the dispersion concentration, nanotube charge, and speed of thermos‐evaporation. Polarized light irradiated through the patterns demonstrates birefringence and confirms the orientation. Scanning electron microscopy and atomic force microscopy show that the nanotubes are aligned along the direction of the wetting lines above 4 wt%, while they are not oriented at lower concentrations. Halloysite concentration, drying temperature, and type of brush fibers affect the pattern ordering. The aligned halloysite systems on glass, tissue culture plates, and polymer films, provide a promising platform for biocell guiding. Human foreskin fibroblasts proliferated well on the aligned clay patterns and the cell orientation agrees with the nanotube direction. Human bone mesenchymal stem cells (HBMSCs) are also cultured on the organized halloysite coating. The clay patterns support HBMSC proliferation with alignment, and such nanostructured substrates promote osteogenesis differentiation without growth factors. This facile method for preparing aligned halloysite patterns on solid substrates is very promising for surface modification in biotissue engineering.

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Aligned carbon nanotube reinforced polymeric scaffolds with electrical cues for neural tissue regeneration

Cited by 89 publications

References 35 publications

In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

Chitosan nanocomposites based on distinct inorganic fillers for biomedical applications

Clay Nanotubes Aligned with Shear Forces for Mesenchymal Stem Cell Patterning

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