Alienimonas chondri sp. nov., a novel planctomycete isolated from the biofilm of the red alga Chondrus crispus

Vitorino, Inês; Albuquerque, Luciana; Wiegand, Sandra; Kallscheuer, Nicolai; Costa, Milton S. da; Lobo‐da‐Cunha, Alexandre; Jogler, Christian; Lage, Olga Maria

doi:10.1016/j.syapm.2020.126083

Cited by 18 publications

(17 citation statements)

References 56 publications

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“…(seventeen isolates) and Porphyra dioica (four isolates). Only one strain was retrieved from Fucus sp., which is affiliated with Rhodopirellula lusitana, and two strains from Chondrus crispus, LzC1 and LzC2 T , which belong to a novel taxon that was recently described based on a polyphasic description study, Alienimonas chondri [57]. In October 2020, one strain closely related to Novipirellula caenicola was retrieved from Ulva sp., and another strain affiliated with R. baltica from Corallina sp.…”

Section: Macroalgae As Source For Planctomycetesmentioning

confidence: 98%

“…The taxa to which all the isolated planctomycetes belong have only been obtained, until present, from marine environments [3,4,6,7,41,57,[66][67][68] (data from the NCBI database, August 2021). In fact, the genus Rhodopirellula, the genus of class Planctomycetia with most isolated members, is well distributed in marine environments and well associated with a variety of macroalgae, which includes diverse species found on the coast facing the Atlantic [3,4,7,10,41,69,70].…”

Section: Ecology Of the Isolated Planctomycetal Speciesmentioning

confidence: 99%

“…Regarding Alienimonas chondri, besides our two isolates retrieved from the macroalga Chondrus crispus (which include the species type strain), there is currently no data on other isolates/uncultured clones in the NCBI database with an affiliation to this species (>98.7% similarity of the 16S rRNA gene) [57].…”

Section: Ecology Of the Isolated Planctomycetal Speciesmentioning

confidence: 99%

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Novel and Conventional Isolation Techniques to Obtain Planctomycetes from Marine Environments

et al. 2021

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Bacteria from the distinctive Planctomycetes phylum are well spread around the globe; they are capable of colonizing many habitats, including marine, freshwater, terrestrial, and even extreme habitats such as hydrothermal vents and hot springs. They can also be found living in association with other organisms, such as macroalgae, plants, and invertebrates. While ubiquitous, only a small fraction of the known diversity includes axenic cultures. In this study, we aimed to apply conventional techniques to isolate, in diverse culture media, planctomycetes from two beaches of the Portuguese north-coast by using sediments, red, green, and brown macroalgae, the shell of the mussel Mytilus edulis, an anemone belonging to the species Actinia equina, and seawater as sources. With this approach, thirty-seven isolates closely related to seven species from the families Planctomycetaceae and Pirellulaceae (class Planctomycetia) were brought into pure culture. Moreover, we applied an iChip inspired in-situ culturing technique to successfully retrieve planctomycetes from marine sediments, which resulted in the isolation of three additional strains, two affiliated to the species Novipirellula caenicola and one to a putative novel Rubinisphaera. This work enlarges the number of isolated planctomycetal strains and shows the adequacy of a novel methodology for planctomycetes isolation.

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Section: Macroalgae As Source For Planctomycetesmentioning

confidence: 98%

Section: Ecology Of the Isolated Planctomycetal Speciesmentioning

confidence: 99%

Section: Ecology Of the Isolated Planctomycetal Speciesmentioning

confidence: 99%

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Novel and Conventional Isolation Techniques to Obtain Planctomycetes from Marine Environments

et al. 2021

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“…A great number of published data has reported that Planctomycetes are frequently associated with the epibacterial community of several algae (Longford et al, 2007;Burke et al, 2011;Lachnit et al, 2011;Lage and Bondoso, 2011;Hollants et al, 2013;Lage and Bondoso, 2014;Jogler et al, 2020;Vitorino et al, 2020). The abundant planctomycete populations on kelp surfaces and in association with other macroalga suggest that coexistence with these eukaryotes may be a key feature of many planctomycete lifestyles.…”

Section: Planctomycetes Associated With Algaementioning

confidence: 99%

Planctomycetes as Host-Associated Bacteria: A Perspective That Holds Promise for Their Future Isolations, by Mimicking Their Native Environmental Niches in Clinical Microbiology Laboratories

Kaboré

Godreuil

Drancourt

2020

Front. Cell. Infect. Microbiol.

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Traditionally recognized as environmental bacteria, Planctomycetes have just been linked recently to human pathology as opportunistic pathogens, arousing a great interest for clinical microbiologists. However, the lack of appropriate culture media limits our future investigations as no Planctomycetes have ever been isolated from patients’ specimens despite several attempts. Several Planctomycetes have no cultivable members and are only recognized by 16S rRNA gene sequence detection and analysis. The cultured representatives are slow-growing fastidious bacteria and mostly difficult to culture on synthetic media. Accordingly, the provision of environmental and nutritional conditions like those existing in the natural habitat where yet uncultured/refractory bacteria can be detected might be an option for their potential isolation. Hence, we systematically reviewed the various natural habitats of Planctomycetes, to review their nutritional requirements, the physicochemical characteristics of their natural ecological niches, current methods of cultivation of the Planctomycetes and gaps, from a perspective of collecting data in order to optimize conditions and the protocols of cultivation of these fastidious bacteria. Planctomycetes are widespread in freshwater, seawater, and terrestrial environments, essentially associated to particles or organisms like macroalgae, marine sponges, and lichens, depending on the species and metabolizable polysaccharides by their sulfatases. Most Planctomycetes grow in nutrient-poor oligotrophic environments with pH ranging from 3.4 to 11, but a few strains can also grow in quite nutrient rich media like M600/M14. Also, a seasonality variation of abundance is observed, and bloom occurs in summer-early autumn, correlating with the strong growth of algae in the marine environments. Most Planctomycetes are mesophilic, but with a few Planctomycetes being thermophilic (50°C to 60°C). Commonly added nutrients are N-acetyl-glucosamine, yeast-extracts, peptone, and some oligo and macro-elements. A biphasic host-associated extract (macroalgae, sponge extract) conjugated with a diluted basal medium should provide favorable results for the success of isolation in pure culture.

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“…16S rRNA gene sequence-based phylogeny was computed for the strains described here and the type strains of reference species. The analysis also included strains described in the last year (Boersma et al 2019;Dedysh et al 2020a;Kallscheuer et al 2020b;Kohn et al 2016Kohn et al , 2019Kohn et al , 2020Kumar et al 2020;Peeters et al 2020;Rivas-Marin et al 2020a, b;Vitorino et al 2020) and strains recently published, but not yet described (Wiegand et al 2020). The 16S rRNA gene sequences were aligned with SINA (Pruesse et al 2012).…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

Additions to the genus Gimesia: description of Gimesia alba sp. nov., Gimesia algae sp. nov., Gimesia aquarii sp. nov., Gimesia aquatilis sp. nov., Gimesia fumaroli sp. nov. and Gimesia panareensis sp. nov., isolated from aquatic habitats of the Northern Hemisphere

Wiegand

Jogler

Boedeker

et al. 2020

Antonie van Leeuwenhoek

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Thirteen novel planctomycetal strains were isolated from five different aquatic sampling locations. These comprise the hydrothermal vent system close to Panarea Island (Italy), a biofilm on the surface of kelp at Monterey Bay (CA, USA), sediment and algae on Mallorca Island (Spain) and Helgoland Island (Germany), as well as a seawater aquarium in Braunschweig, Germany. All strains were shown to belong to the genus Gimesia. Their genomes cover a size range from 7.22 to 8.29 Mb and have a G+C content between 45.1 and 53.7%. All strains are mesophilic (Topt 26–33 °C) with generation times between 12 and 32 h. Analysis of fatty acids yielded palmitic acid (16:0) and a fatty acid with the equivalent chain length of 15.817 as major compounds. While five of the novel strains belong to the already described species Gimesia maris and Gimesia chilikensis, the other strains belong to novel species, for which we propose the names Gimesia alba (type strain Pan241wT = DSM 100744T = LMG 31345T = CECT 9841T = VKM B-3430T), Gimesia algae (type strain Pan161T = CECT 30192T = STH00943T = LMG 29130T), Gimesia aquarii (type strain V144T = DSM 101710T = VKM B-3433T), Gimesia fumaroli (type strain Enr17T = DSM 100710T = VKM B-3429T) and Gimesia panareensis (type strain Enr10T = DSM 100416T = LMG 29082T). STH numbers refer to the Jena Microbial Resource Collection (JMRC).

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Alienimonas chondri sp. nov., a novel planctomycete isolated from the biofilm of the red alga Chondrus crispus

Cited by 18 publications

References 56 publications

Novel and Conventional Isolation Techniques to Obtain Planctomycetes from Marine Environments

Novel and Conventional Isolation Techniques to Obtain Planctomycetes from Marine Environments

Planctomycetes as Host-Associated Bacteria: A Perspective That Holds Promise for Their Future Isolations, by Mimicking Their Native Environmental Niches in Clinical Microbiology Laboratories

Additions to the genus Gimesia: description of Gimesia alba sp. nov., Gimesia algae sp. nov., Gimesia aquarii sp. nov., Gimesia aquatilis sp. nov., Gimesia fumaroli sp. nov. and Gimesia panareensis sp. nov., isolated from aquatic habitats of the Northern Hemisphere

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