Alien Species of Fish in the Littoral of Volga and Kama Reservoirs (Results of Complex Expeditions of IBIW RAS in 2005-2017)

Karabanov, D. P.; Pavlov, D. F.; Bazarov, M. I.; Боровикова, Е. А.; Yu., V Gerasimov; Kodukhova, Yu. V.; Smirnov, A. K.; Столбунов, И. А.

doi:10.1101/355362

Cited by 9 publications

(10 citation statements)

References 35 publications

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“…The sampling was carried out during coastal fish surveys in the water bodies and watercourses of Central Russia, mainly in the Volga-Kama basin [17] in accordance with the state permit issued to the IBIW RAS for catching biological resources. Some samples were provided by regional environmental inspectors.…”

Section: Samplingmentioning

confidence: 99%

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Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region)

Karabanov¹,

Ei²,

Dd³

et al. 2021

Preprint

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Reliable species identification is critical for detection and monitoring of biological invasions. In this study, we propose four sets of primers for efficient amplification of several loci, including the mitochondrial cytochrome oxidase-c (COI) subunit I gene which is a basis for DNA barcoding. This set of primers gives a shorter product which can be used in high-throughput sequencing systems for metabarcoding purposes. Another mitochondrial locus encoding the large ribosomal subunit (16S) may be useful to study the population structure and as an additional source of information in the metabarcoding of communities. We propose to use a set of primers for the nuclear locus of the small ribosomal subunit (18S) as a positive control and to verify the results of the barcoding. Our proposed sets of primers demonstrate a high amplification efficiency and a high specificity both for freshwater alien and indigenous fishes. The proposed research design makes it possible to carry out extremely cheap studies on the assessment of biological diversity using genetic analysis without expensive equipment, and with the technique for conducting laboratory work and processing of the results available to any researcher. The paper also presents original data on the genetic polymorphism of all mass alien fish species in the Volga-Kama region. High efficiency of DNA identification based on our primers is shown as compared to traditional monitoring of biological invasions.

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Section: Samplingmentioning

confidence: 99%

“…Currently, 11 alien fish species are found in mass in the Volga-Kama region (the largest in Europe), and another ten are represented by single findings or local populations [17]. All common species are studied here, however, different efficiency of DNA identification may be noted for different taxa.…”

Section: Dna Manipulation Amplification and Sequencingmentioning

confidence: 99%

Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region)

Karabanov¹,

Ei²,

Dd³

et al. 2021

Preprint

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“…Since the 1950-60s, non-indigenous hydrobionts became common in the Volga basin [19,27,28], and NIS of fishes have been detected during 1970-80. All previous records were summarized and discussed in publications appearing only in the 21st century [19,25,29].…”

Section: Introductionmentioning

confidence: 99%

New Sets of Primers for DNA Identification of Non-Indigenous Fish Species in the Volga-Kama Basin (European Russia)

Karabanov

Bekker

Pavlov

et al. 2022

Water

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Adequate species’ identification is critical for the detection and monitoring of biological invasions. In this study, we proposed and assessed the efficiency of newly created primer sets for the genetic identification of non-indigenous species (NIS) of fishes in the Volga basin based on: (a) a “long” fragment of cytochrome c oxidase subunit one of the mitochondrial gene (COI) (0.7 kb), used in “classical” DNA barcoding; (b) a short 3’-fragment (0.3 kb) of COI, suitable for use in high-throughput sequencing systems (i.e., for dietary analysis); (c) fragment of 16S mitochondrial rRNA, including those designed to fill the library of reference sequences for work on the metabarcoding of communities and eDNA studies; (d) a fragment of 18S nuclear rRNA, including two hypervariable regions V1-V2, valuable for animal phylogeny. All four sets of primers demonstrated a high amplification efficiency and high specificity for freshwater fish. Also, we proposed the protocols for the cost-effective isolation of total DNA and purification of the PCR product without the use of commercial kits. We propose an algorithm to carry out extremely cheap studies on the assessment of biological diversity without expensive equipment. We also present original data on the genetic polymorphism of all mass NIS fish species in the Volga-Kama region. The high efficiency of DNA identification based on our primers is shown relative to the traditional monitoring of biological invasions.

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“…Common bream Abramis brama is one of the most abundant species of Cyprinidae family in the water reservoirs of the Volga-Caspian Basin. The species has an important place in the ecosystem of water bodies and is considered an important object of commercial and sport fishing (Subbotkin & Subbotkina, 2016;Gerasimov et al, 2018;Karabanov et al, 2018). Therefore, study of the content of metabolic products in the muscles and organs of bream that live in the conditions of the water reservoirs of the Upper Volga is of great interest for solving a number of tasks in hydrobiology.…”

Section: Introductionmentioning

confidence: 99%

Impact of habitation conditions on metabolism in the muscles, liver, and gonads of different sex and age groups of bream

Payuta

Флерова

2021

Regul. Mech. Biosyst.

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Impact of the factors of the aquatic environment is an inevitable aspect of the life of fish as poikilothermic animals and provokes responses in their organisms. The study focused on determining peculiarities in the composition of the metabolic products in the tissues of different age and sex groups of common bream Abramis brama (L.) depending on the living conditions in the water reservoirs of the Upper Volga. The fish were captured in the fattening period in summer and autumn, measured, weighed, identifying sex, maturity stage of the gonads and age. In the muscles, liver and gonads of bream, we analyzed the contents of water, dry matter, lipids, protein, ash and carbohydrates using the standard techniques. The contents of biochemical components in the organism of bream were to a higher degree determined by the peculiarities of the living conditions rather than sex and age of the individuals. In the muscles and liver of bream living in the conditions of increased water temperature and low concentration of oxygen, the concentrations of protein and ash were lower. In the same tissues of bream from the water bodies with high concentrations of pollutants in the water and benthic deposits, we found increased fat content. The muscles of male bream contained less protein and more carbohydrates than females and juvenile individuals, and the muscles of juveniles had less fat. The environmental factors had greater influence on the content of the metabolic products in the liver of bream than sex, and only females had higher ash content than males. The testes had more fat and less protein than the ovaries. During the transition of the gonads from maturity stage II to III, the contents of dry matter and lipids in them decreased. We determined that the environmental factors have stronger impact on biochemical parameters in the tissues of bream than age, because we found no general age patterns in the dynamics of biochemical components, except the liver, in which the highest content of organic compounds was found in individuals aged 6 to 10 years. Water heated by the Hydro Power Plant positively influenced the young bream, as indicated by higher content of metabolic products in individuals aged 5+ than older groups and bream from the surveyed water bodies. Monitoring of the physiological condition of bream focusing on the contents of metabolic products in the tissues may provide necessary data for successful regulation of bream populations in the water reservoirs of the Upper Volga.

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Alien Species of Fish in the Littoral of Volga and Kama Reservoirs (Results of Complex Expeditions of IBIW RAS in 2005-2017)

Cited by 9 publications

References 35 publications

Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region)

Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region)

New Sets of Primers for DNA Identification of Non-Indigenous Fish Species in the Volga-Kama Basin (European Russia)

Impact of habitation conditions on metabolism in the muscles, liver, and gonads of different sex and age groups of bream

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