Alien genes introgression and development of alien monosomic addition lines from a threatened species, Allium roylei Stearn, to Allium cepa L.

Vu, Hoa Q.; Yoshimatsu, Yasuyuki; Khrustaleva, Ludmila; Yamauchi, Naoki; Shigyo, Masayoshi

doi:10.1007/s00122-011-1783-5

Cited by 17 publications

(9 citation statements)

References 51 publications

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“…, Vu et al. ). These species can be utilized to develop onion cultivars resistant against various diseases along with desirable agronomic traits, such as earliness and better rooting system.…”

Section: Breeding Achievements and Challengesmentioning

confidence: 99%

Enhancing onion breeding using molecular tools

et al. 2015

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Bulb onion (Allium cepa L.) is an ancient crop that is thought to have originated in Central Asia and has been cultivated for over 5000 years. Classical genetic and plant breeding approaches have been used to improve onion yield, quality, and resistance against biotic and abiotic stresses. However, its biennial life cycle, cross-pollinated nature and high inbreeding depression have proved challenging for the characterization and breeding of improved traits. New technologies, notably next-generation sequencing, are providing researchers with the genomic resources and approaches to overcome these challenges. Using these genomic technologies, molecular markers are being rapidly developed and utilized for germplasm analysis and mapping in onion. These new tools and knowledge are allowing the integration of molecular and conventional breeding to speed up onion improvement programmes. In this review, we outline recent progress in onion genomics and molecular genetics and prospects for enhancing onion yield and quality in the future.

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Section: Breeding Achievements and Challengesmentioning

confidence: 99%

Enhancing onion breeding using molecular tools

et al. 2015

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“…9) In continuation of our previous research, 10) a comprehensive quantitative and qualitative analysis of cysteine sulfoxides, flavonols, polyphenols, ascorbic acid, and saponins within different organs of A. roylei was conducted. Localization of saponins and flavonoids in A. roylei organs was explored for the first time in Allium species together with an assay of antioxidant activity.…”

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confidence: 99%

Compartmentation and localization of bioactive metabolites in different organs of Allium roylei

Abdelrahman

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et al. 2014

Bioscience, Biotechnology, and Biochemistry

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To investigate the involvement of Allium roylei metabolites in the plant's defenses, a comprehensive analysis of the content of cysteine sulfoxides, flavonols, polyphenols, ascorbic acid, and saponins was carried out in the various organs of this species. Metabolomics high performance liquid chromatography (HPLC), spectral-based analysis, and histochemcial studies have given important insight to the validity of saponins as a key component involved in plant protection. The root-basal stem, bulb, and leaf extracts exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity with inhibition concentration (IC 50 ) ranging from 0.649 to 0.757 mg/mL. The antimicrobial properties of the saponin and flavonoid crude extracts were evaluated. The saponin extracts demonstrated significant antifungal activity depending on the applied concentration, and the growth inhibition rate of the tested fungal pathogens ranged from 1.07 to 47.76%. No appreciable antibacterial activity was recorded in the same sample.Key words: cysteine sulfoxides; flavonoids; saponins; antioxidant activity; antimicrobial activityThe need for disease-and pest-resistant germplasm in onion (Allium cepa L.) breeding has promoted studies of the biosystematic relationships between edible and wild Allium species of the section Cepa.1) Domestication of wild Allium species started millennia ago, followed by extensive dissemination of the flavoring condiment all over the world.2) Many wild Allium species with the characteristic onion or garlic aroma are used as spices or vegetables by various folks.3) The wild Allium species may serve as a potential source for Allium crop improvement, and it may comprise genes for desired traits, as in Allium roylei Stearn. 4) A. roylei has proven to be a crucial bridge species, enabling introgressions of genetic resources from other related species of section Cepa into the onion, allowing disease resistance and other valuable characteristics to be introgressed into the onion.5) The prospect of exploiting A. roylei as a source of disease resistance in onion breeding is very promising, as this wild species has proven to be completely resistant to downy mildew (Perenospora destructor), and partially resistant to leaf blight disease caused by Botrytis squamosa and basal rot disease caused by Fusarium oxysporum f.sp. cepae. 6)Although edible Allium species are rich in numerous phytonutrients and bioactive metabolites (cysteine sulfoxides (ACSO), flavonoids, polyphenols, saponins, etc.) which reportedly have many functional properties such as antimicrobial, anticancer, and antioxidant, 7,8) little effort has been invested in evaluating the wild Allium species as potential sources for these bioactive metabolites with therapeutic and disease resistance properties.There is no available information concerning the distribution and localization of these bioactive metabolites in different organs of A. roylei and their role as a part of the defensive characteristics of the plant against F. oxysporum f.sp cepae. Therefore, under...

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“…8R). The recombination probably resulted from the chiasma formation during the meiosis of the allotriploid as also reported by Vu et al (2012). Further GISH analyses are required to reveal the genomic constitutions and recombination frequencies of all of the BC 2 plants.…”

Section: Discussionmentioning

confidence: 90%

“…In contrast, backcrossings of A. cepa-A. roylei allotriploids had relatively high rates of germinated seeds (Vu et al 2012). We did not succeed in completing the eight possible types of AMALs, but we found that AMALs with 2n = 17 appeared with the highest frequency among the BC 2 plants.…”

Section: Discussionmentioning

confidence: 94%

“…Doubling of the sterile F 1 hybrid is one way to overcome these barriers (Singh 2003). In a previous study, a high number of alien addition lines of A. cepa carrying extra chromosomes from A. roylei were produced by backcrossing the doubled F 1 hybrid (Vu et al 2012). Alien addition lines, which carry the extra chromosomes of wild species and the normal chromosome complement of recipient species, would speed up the introgression process of the wild species by producing chromosome substitution and translocation lines (Singh 2003).…”

Section: Introductionmentioning

confidence: 99%

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