Alicyclobacillus spp.: New Insights on Ecology and Preserving Food Quality through New Approaches

Ciuffreda, Emanuela; Bevilacqua, Antonio; Sinigaglia, Milena; Corbo, Maria Rosaria

doi:10.3390/microorganisms3040625

Cited by 31 publications

(20 citation statements)

References 91 publications

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“…They have been isolated everywhere from the upper layers of the atmosphere to the depths of ocean trenches, and from almost every conceivable environment in between (Fajardo‐Cavazos et al ., ; Zeigler and Perkins, ). Members include not only mesophiles, but acidophiles (Ciuffreda et al ., ), alikaliphiles (Preiss et al ., ), halophiles (Hanelt and Muller, ), psychrophiles (Larkin and Stokes, ), thermophiles (Zeigler, ; Hussein et al ., ) and UV‐radiation tolerant organisms (Ordonez et al ., ). Their physiological capabilities suggest they play a significant role in global carbon, nitrogen and sulphur cycles and in the degradation of plant, fungus and insect biomass (Mandic‐Mulec and Prosser, ).…”

Section: Introductionmentioning

confidence: 99%

Experimental evolution of Bacillus subtilis

Zeigler

Nicholson

2017

Environmental Microbiology

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Summary The endospore‐forming bacteria have persisted on earth perhaps 3Ga, leveraging the flexibility of their distinctive lifestyle to adapt to a remarkably wide range of environments. This process of adaptation can be investigated through the simple but powerful technique of laboratory evolution. Evolved strains can be analyzed by whole genome sequencing and an array of omics technologies. The intensively studied, genetically tractable endospore‐former, Bacillus subtilis, is an ideal subject for laboratory evolution experiments. Here, we describe the use of the B. subtilis model system to study the adaptation of these bacteria to reduced and stringent selection for endospore formation, as well as to novel environmental challenges of low atmospheric pressure, high ultraviolet radiation, and unfavourable growth temperatures. In combination with other approaches, including comparative genomics and environmental field work, laboratory evolution may help elucidate how these bacteria have so successfully adapted to life on earth, and perhaps beyond.

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Section: Introductionmentioning

confidence: 99%

Experimental evolution of Bacillus subtilis

Zeigler

Nicholson

2017

Environmental Microbiology

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“…Members of Alicyclobacillus , Anaerovirgula and Geobacillus (Firmicutes) were also enriched in the earthworm-amended soil and some of their members are characterized by cellulolytic activity. Alicyclobacillus consists of a group of thermo-acidophilic, strictly aerobic, heterotrophic and spore-forming bacteria (Ciuffreda et al, 2015) with some members with cellulolytic capacity, for example, Alicyclobacillus cellulosilyticus (Kusube et al, 2014). Anaerovirgula is an alkaliphilic, cellulolytic obligate anaerobic bacterium (Porsch et al, 2015), while Geobacillus produces thermostable hemicellulose hydrolytic enzymes (De Maayer et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

TiO₂ nanoparticles affect the bacterial community structure and Eisenia fetida (Savigny, 1826) in an arable soil

Sánchez-López

Santos-Ramos

Gómez-Acata

et al. 2019

PeerJ

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The amount of nanoparticles (NP), such as TiO2, has increased substantially in the environment. It is still largely unknown, however, how NP might interact with earthworms and organic material and how this might affect the bacterial community structure and their functionality. Therefore, an arable soil was amended with TiO2 NP at 0, 150 or 300 mg kg−1 and subjected to different treatments. Treatments were soil amended with ten earthworms (Eisenia fetida (Savigny, 1826)) with fully developed clitellum and an average fresh mass of 0.5 to 500 g dry soil, 1.75 g tyndallized Quaker® oat seeds Avena sativa (L.) kg−1, or earthworms plus oat seeds, or left unamended. The bacterial community structure was monitored throughout the incubation period. The bacterial community in the unamended soil changed over time and application of oats, earthworm and a combination of both even further, with the largest change found in the latter. Application of NP to the unamended soil and the earthworm-amended soil altered the bacterial community, but combining it by adding oats negated that effect. It was found that the application of organic material, that is, oats, reduced the effect of the NP applied to soil. However, as the organic material applied was mineralized by the soil microorganisms, the effect of NP increased again over time.

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“…The genus has been named Alicyclobacillus since 1992 to include several isolates previously wrongly attributed to the Bacillus genus (Wisotzkey et al, 1992). Alicyclobacillus are strictly aerobic thermo-acidophilic microorganisms that produce ω-alicyclic fatty acids as major cellular fatty acids (Hippchen et al, 1981;Poralla and König, 1983) and grow optimally at temperatures between 28 and 65 • C (Baumgart, 2003;Ciuffreda et al, 2015). After their discovery, a high number of enzymes have been characterized from those microbes that helped to define the molecular determinants of thermostability (Bartolucci et al, 1997;Atalah et al, 2019).…”

Section: Site Description Sampling and Identification Of A New Strain Of Alicyclobacillusmentioning

confidence: 99%

Genomic Insight of Alicyclobacillus mali FL18 Isolated From an Arsenic-Rich Hot Spring

et al. 2021

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Extreme environments are excellent places to find microorganisms capable of tolerating extreme temperature, pH, salinity pressure, and elevated concentration of heavy metals and other toxic compounds. In the last decades, extremophilic microorganisms have been extensively studied since they can be applied in several fields of biotechnology along with their enzymes. In this context, the characterization of heavy metal resistance determinants in thermophilic microorganisms is the starting point for the development of new biosystems and bioprocesses for environmental monitoring and remediation. This work focuses on the isolation and the genomic exploration of a new arsenic-tolerant microorganism, classified as Alicyclobacillus mali FL18. The bacterium was isolated from a hot mud pool of the solfataric terrains in Pisciarelli, a well-known hydrothermally active zone of the Campi Flegrei volcano near Naples in Italy. A. mali FL18 showed a good tolerance to arsenite (MIC value of 41 mM), as well as to other metals such as nickel (MIC 30 mM), cobalt, and mercury (MIC 3 mM and 17 μM, respectively). Signatures of arsenic resistance genes (one arsenate reductase, one arsenite methyltransferase, and several arsenite exporters) were found interspersed in the genome as well as several multidrug resistance efflux transporters that could be involved in the export of drugs and heavy metal ions. Moreover, the strain showed a high resistance to bacitracin and ciprofloxacin, suggesting that the extreme environment has positively selected multiple resistances to different toxic compounds. This work provides, for the first time, insights into the heavy metal tolerance and antibiotic susceptibility of an Alicyclobacillus strain and highlights its putative molecular determinants.

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Alicyclobacillus spp.: New Insights on Ecology and Preserving Food Quality through New Approaches

Cited by 31 publications

References 91 publications

Experimental evolution of Bacillus subtilis

Experimental evolution of Bacillus subtilis

TiO₂ nanoparticles affect the bacterial community structure and Eisenia fetida (Savigny, 1826) in an arable soil

Genomic Insight of Alicyclobacillus mali FL18 Isolated From an Arsenic-Rich Hot Spring

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Alicyclobacillus spp.: New Insights on Ecology and Preserving Food Quality through New Approaches

Cited by 31 publications

References 91 publications

Experimental evolution of Bacillus subtilis

Experimental evolution of Bacillus subtilis

TiO2 nanoparticles affect the bacterial community structure and Eisenia fetida (Savigny, 1826) in an arable soil

Genomic Insight of Alicyclobacillus mali FL18 Isolated From an Arsenic-Rich Hot Spring

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TiO₂ nanoparticles affect the bacterial community structure and Eisenia fetida (Savigny, 1826) in an arable soil