. Developing a stochastic dynamic programming framework for optical tweezers based automated particle transport operations. IEEE Transactions on Automation Science and Engineering, 7(2), 218 -227, 2010. Readers are encouraged to get the official version from the journal's web site or by contacting Dr. S.K. Gupta (skgupta@umd.edu). Ashis Gopal Banerjee, Student Member, IEEE, Andrew Pomerance, Wolfgang Losert, and Satyandra K. GuptaAbstract-Automated particle transport using optical tweezers requires the use of motion planning to move the particle while avoiding collisions with randomly moving obstacles. This paper describes a stochastic dynamic programming based motion planning framework developed by modifying the discrete version of an infinite-horizon partially observable Markov decision process algorithm. Sample trajectories generated by this algorithm are presented to highlight effectiveness in crowded scenes and flexibility. The algorithm is tested using silica beads in a holographic tweezer set-up and data obtained from the physical experiments are reported to validate various aspects of the planning simulation framework. This framework is then used to evaluate the performance of the algorithm under a variety of operating conditions.Note to Practitioners-Micro and nano scale component-based devices are revolutionizing health care, energy, communication, and computing industry. Components need to be assembled together to create useful devices. Such assembly operations remain challenging in spite of the advancements in imaging, measurement, and fabrication at the small scales. This paper deals with directed assembly using optical fields that is useful for prototyping new design concepts, repairing devices, and creating templates for self-assembly.