Alginate-Templated Microcapsules Carrying Heparin via Layer-by-Layer Self-Assembly

Li, Juan; Liu, Xinrong; Zhu, Baoqing; Shao, Yingya; Zhao, Dongxu; Yang, Xinlin

doi:10.1109/icbbe.2008.684

Cited by 1 publication

(1 citation statement)

References 16 publications

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“…ChOx from Streptomyces sp. was chemically coupled by Carbodi‐imide within the spheres and layer‐by‐layer (LBL) self‐assembly was used for enzyme leaching control (Brown et al, 2005; Li et al, 2008; Lolekha et al, 2004; Pollegioni et al, 1999; Srivastava et al, 2005; Zhu et al, 2005b). Co‐entrapment of cholesterol esterase was not performed out as it leads to a very slow reaction and a slow response; therefore, any cholesterol present in esterified form needs to be pretreated with free cholesterol esterase to derive the free cholesterol for sensing application.…”

Section: Introductionmentioning

confidence: 99%

Cholesterol biosensors based on oxygen sensing alginate‐silica microspheres

Prasad¹,

Joshi²,

Jayant³

et al. 2011

Biotech & Bioengineering

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Cholesterol determination in body is important in diagnosis of diseases like coronary heart disease, arteriosclerosis, diabetes, and obstructive jaundice. This research aims at developing fluorimetric cholesterol biosensors based on self-assembled mesoporous alginate-silica (Algilica) microspheres. For preparing the biosensor, Pt-(II)-octaethylporphine (PtOEP; oxygen sensitive metalloporphyrin) dye has been loaded in the Algilica microspheres using the solvent-mediated precipitation method. Cholesterol oxidase (ChOx) was then covalently conjugated to PtOEP/Algilica microspheres using EDC and NHS reagents. PtOEP dye and enzyme encapsulation, activity and stability were then analyzed. Layer-by-layer self-assembly was finally performed using PAH and PSS polyelectrolytes to minimize leaching of the biosensor components. The prepared biosensor exhibited linearity over a range of 0.77-2.5 mM O(2) (K(SV) : 0.097/mM of O(2) ) obtained using from Stern-Volmer plots. The biosensor response to standard cholesterol displayed a linear analytical range from 1.25 to 10 mM of cholesterol with regression coefficient of 0.996 (1.25-3.75 mM), 0.976 (1.25-6 mM), and 0.959 (1.25-10 mM) and response time of 10 min. Thus, the prepared cholesterol biosensor shows great potential in the diagnosis of hypercholesterolemia.

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