Alginate production by clinical nonmucoid Pseudomonas aeruginosa strains

Anastassiou, E.D.; Mintzas, Anastassios C.; Kounavis, C; Dimitracopoulos, G.

doi:10.1128/jcm.25.4.656-659.1987

Cited by 53 publications

(21 citation statements)

References 30 publications

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“…After centrifugation (14,000 rpm, 10 min), 50 ml of the obtained supernatant was precipitated using 150 ml of cold absolute ethanol (Sigma Aldrich, USA), 10% sodium acetate, and 1% glacial acetic acid (Sigma Aldrich, USA). The alginate pellet was then freeze dried (Anastassiou, Mintzas, Kounavis, & Dimitracopoulos, ).…”

Section: Methodsmentioning

confidence: 99%

Wound healing property of a gel prepared by the combination ofPseudomonas aeruginosaalginate andAlhagi maurorumaqueous extract in rats

Pourali

Yahyaei

2018

Dermatologic Therapy

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Although alginate has been known to be a good wound dressing, it does not have antimicrobial properties, has low availability, and is expensive. To overcome these problems, the present study was conducted, where the extraction of this material from an available small factory Pseudomonas aeruginosa and the improvement of its wound healing property by its combination with herb extract, Alhagi maurorum, done. Nineteen P. aeruginosa strains were isolated and identified from burned skin, and the one isolated strain with the highest ability of alginate production was selected. A. maurorum aqueous extract was prepared, and the toxicity of each material was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide assay. A mixture of nontoxic doses of each substance was then prepared. Thirty-two Wistar rats were divided into four groups (n = 8). The control group and the rest three groups, which were treated by alginate, A. maurorum extract, and alginate-A. maurorum extract. Throughout the 21 days of treatment, the open wound sites were checked. Finally, the rats were sacrificed and the effect of each substance on their skin tissue was evaluated. The results showed that the high alginate production without any toxic effect was obtained from the P. aeruginosa strain K1. A. maurorum aqueous extract had dose-dependent toxicity. The aqueous solution of alginate-A. maurorum extract complex group showed the best wound healing activity in both macroscopic and microscopic examinations. Recent research has introduced a new type of wound dressing with high wound healing properties. This could decrease the time for re-epithelialization and increase wound contraction percentage. K E Y W O R D Salginate, Alhagi maurorum, Pseudomonas aeruginosa, wound healing

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Section: Methodsmentioning

confidence: 99%

Wound healing property of a gel prepared by the combination ofPseudomonas aeruginosaalginate andAlhagi maurorumaqueous extract in rats

Pourali

Yahyaei

2018

Dermatologic Therapy

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“…The P. aeruginosa strains that initially infect these patients are generally non-mucoid and sensitive to antibiotics (Doggett et al, 1966;Burns et al, 2001). Phenotypically non-mucoid P. aeruginosa strains, when cultured in vitro, produce low levels of alginate (Pier et al, 1986;Anastassiou et al, 1987). CF patients that only have non-mucoid strains infecting their lungs have antibody responses specific for alginate (Pedersen et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Proteolytic regulation of alginate overproduction in Pseudomonas aeruginosa

Damron

Goldberg

2012

Molecular Microbiology

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Summary Pseudomonas aeruginosa, a Gram‐negative bacterium, is a significant opportunistic pathogen associated with skin and soft tissue infections, nosocomial pneumonia and sepsis. In addition, it can chronically colonize the lungs of cystic fibrosis (CF) patients. Overproduction of the exopolysaccharide called alginate provides P. aeruginosa with a selective advantage and facilitates survival in the CF lung. The in vitro phenotype of alginate overproduction observed on solid culture media is referred to as mucoid. Expression of the alginate machinery and biosynthetic enzymes are controlled by the extracytoplasmic sigma factor, σ22 (AlgU/T). The key negative regulator of both σ22 activity and the mucoid phenotype is the cognate anti‐sigma factor MucA. MucA sequesters σ22 to the inner membrane inhibiting the sigma factor's transcriptional activity. The well‐studied mechanism for transition to the mucoid phenotype is mutation of mucA, leading to loss of MucA function and therefore activation of σ22. Recently, regulated intramembrane proteolysis (RIP) has been recognized as a mechanism whereby proteolysis of the anti‐sigma factor MucA leads to active σ22 allowing P. aeruginosa to respond to environmental stress conditions by overproduction of alginate. The goal of this review is to illuminate the pathways leading to RIP that have been identified and proposed.

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“…; Franklin and Ohman ) which are typical for uronic acid containing polymers (Anastassiou et al . ). In FT/IR spectra of alkali‐treated alginate, no bands were observed at 1250 and 1735 cm −1 from ester bonds in acetyl groups (Fig.…”

Section: Discussionmentioning

confidence: 97%