Alginate oligosaccharide alleviates D‐galactose‐induced cardiac ageing via regulating myocardial mitochondria function and integrity in mice

Abstract

Ageing is a crucial risk factor for the development of age‐related cardiovascular diseases. Therefore, the molecular mechanisms of ageing and novel anti‐ageing interventions need to be deeply studied. Alginate oligosaccharide (AOS) possesses high pharmacological activities and beneficial effects. Our study was undertaken to investigate whether AOS could be used as an anti‐ageing drug to alleviate cardiac ageing. D‐galactose (D‐gal)‐induced C57BL/6J ageing mice were established by subcutaneous injection of D‐gal (200 mg·kg‐1·d‐1) for 8 weeks. AOS (50, 100 and 150 mg·kg‐1·d‐1) were administrated intragastrically for the last 4 weeks. As a result, AOS prevented cardiac dysfunction in D‐gal‐induced ageing mice, including partially preserved ejection fraction (EF%) and fractional shortening (FS%). AOS inhibited D‐gal‐induced up‐regulation of natriuretic peptides A (ANP), brain natriuretic peptide (BNP) and ageing markers p53 and p21 in a dose‐dependent manner. To further explore the potential mechanisms contributing to the anti‐ageing protective effect of AOS, the age‐related mitochondrial compromise was analysed. Our data indicated that AOS alleviated D‐gal‐induced cardiac ageing by improving mitochondrial biogenesis, maintaining the mitochondrial integrity and enhancing the efficient removal of impaired mitochondria. AOS also decreased the ROS production and oxidative stress status, which, in turn, further inhibiting cardiac mitochondria from being destroyed. Together, these results demonstrate that AOS may be an effective therapeutic agent to alleviate cardiac ageing.

Silencing MR-1 Protects against Myocardial Injury Induced by Chronic Intermittent Hypoxia by Targeting Nrf2 through Antioxidant Stress and Anti-Inflammation Pathways

“…Numerous animal models have been developed for the study of hypoxia, of which the most widely used is the CIH model, which simulates the intermittent hypoxia that occurs in OSAHS [ 18 , 19 ]. Heart damage, the HW/BW ratio, or levels of BNP and ANP have been reported to be significantly increased in CIH animals or OSA patients [ 9 , 20 ]. Our experimental results are consistent with this research data and showed that silencing MR-1 could reduce the indices of CIH-induced heart failure.…”

Patchouli Alcohol Inhibits D-Gal Induced Oxidative Stress and Ameliorates the Quality of Aging Cartilage via Activating the Nrf2/HO-1 Pathway in Mice

“…D-galactose- (D-gal-) induced aging animal model has been widely applied to study the senescence mechanism and antiaging efficacy of drugs [ 15 ]. It is considered as an agent for inducing animal senescence for its ability to accumulate ROS, which further brings about a physiological state similar to aging, consisting of decreased antioxidant activity, increased free radical level, and enhanced oxidative stress level in all organs of the organism [ 16 , 17 ]. Pogostemon cablin is a Chinese herbal medicine with the functions of dispelling dampness and removing heat and external syndrome [ 18 ].…”

Nobiletin Prevents D-Galactose-Induced C2C12 Cell Aging by Improving Mitochondrial Function

“…In order to understand the underlying mechanisms of Nob for the prevention of D-gal-induced C2C12 cell aging, we examined the signaling pathway involved in autophagy-dependent catabolism. AMPK-activated kinase (AMPK) has a vital role in the regulation of autophagy [ 28 , 29 ]. Therefore, we evaluated the expression of phosphorylated AMPK at Thr172 (p-AMPK) and AMPK in CK and D-gal-induced C2C12 myoblasts treated with DMSO or Nob.…”