Alginate Nanoparticles Enhance Anti-Clostridium perfringens Activity of the Leaderless Two-Peptide Enterocin DD14 and Affect Expression of Some Virulence Factors

Zgheib, Hassan; Belguesmia, Yanath; Boukherroub, Rabah; Drider, Djamel

doi:10.1007/s12602-020-09730-y

Cited by 12 publications

(15 citation statements)

References 58 publications

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“…Indeed, lacticaseicin 30 or lacticaseicin 30+ colistin enabled a clear downregulation of mcr -1 or mcr -9 expression transcript. Similar effects were observed by Zgheib et al [ 61 ] and Belguesmia et al [ 59 ] when assessing the impact of EntDD14 on the expression of genes coding for virulence factors in Clostridium perfringens and MRSA-SA1 strains. From the twelve strains considered in this study, ten possess the plasmidic mcr-1 gene, sharing common regulation features [ 62 ].…”

Section: Discussionsupporting

confidence: 82%

Lacticaseicin 30 and Colistin as a Promising Antibiotic Formulation against Gram-Negative β-Lactamase-Producing Strains and Colistin-Resistant Strains

et al. 2021

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Antimicrobial resistance is a global health concern across the world and it is foreseen to swell if no actions are taken now. To help curbing this well announced crisis different strategies are announced, and these include the use of antimicrobial peptides (AMP), which are remarkable molecules known for their killing activities towards pathogenic bacteria. Bacteriocins are ribosomally synthesized AMP produced by almost all prokaryotic lineages. Bacteriocins, unlike antibiotics, offer a set of advantages in terms of cytotoxicity towards eukaryotic cells, their mode of action, cross-resistance and impact of microbiota content. Most known bacteriocins are produced by Gram-positive bacteria, and specifically by lactic acid bacteria (LAB). LAB-bacteriocins were steadily reported and characterized for their activity against genetically related Gram-positive bacteria, and seldom against Gram-negative bacteria. The aim of this study is to show that lacticaseicin 30, which is one of the bacteriocins produced by Lacticaseibacillus paracasei CNCM I-5369, is active against Gram-negative clinical strains (Salmonella enterica Enteritidis H10, S. enterica Typhimurium H97, Enterobacter cloacae H51, Escherichia coli H45, E. coli H51, E. coli H66, Klebsiella oxytoca H40, K. pneumoniae H71, K. variicola H77, K. pneumoniae H79, K. pneumoniae H79), whereas antibiotics failed. In addition, lacticaseicin 30 and colistin enabled synergistic interactions towards the aforementioned target Gram-negative clinical strains. Further, the combinations of lacticaseicin 30 and colistin prompted a drastic downregulation of mcr-1 and mcr-9 genes, which are associated with the colistin resistance phenotypes of these clinical strains. This report shows that lacticaseicin 30 is active against Gram-negative clinical strains carrying a rainbow of mcr genes, and the combination of these antimicrobials constitutes a promising therapeutic option that needs to be further exploited.

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Section: Discussionsupporting

confidence: 82%

Lacticaseicin 30 and Colistin as a Promising Antibiotic Formulation against Gram-Negative β-Lactamase-Producing Strains and Colistin-Resistant Strains

et al. 2021

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“…EntDD14 was purified to homogeneity, using a two-step procedure described by Zgheib et al [ 14 ]. E. faecalis 14 was grown in 100 mL of M17 broth (Sigma-Aldrich, St Louis, MO, USA), supplemented with 0.5% of glucose and buffered with 60 mM sodium phosphate (pH 6.3), at 37 °C for 24 h. After harvesting by centrifugation (8,000 rpm, 4 °C, 10 min), the cell-free supernatant was incubated 24 h with 160 rpm continuous shaking at room temperature with 3 g of CM Sephadex® C-25 resin (GE Healthcare Life Sciences, Chicago, IL, USA) previously suspended in 30 mL of distilled water and equilibrated for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…The culture medium was changed every two days to maintain optimal conditions for cell growth. Then obtained Caco-2 cells monolayers were pre-stimulated with lipopolysaccharide (LPS) from E. coli (Sigma-Aldrich, St Louis, MO, USA) (50 μg/mL) for 36 h. Then, the EntDD14 was added, in triplicate, at two different concentrations (60 μg/mL and 240 μg/mL) which were previously tested [ 13 , 14 ], dexamethasone (20 mM) (Sigma-Aldrich, St Louis, MO, USA) as positive control and DMEM containing LPS (50 μg/mL) as negative control. The plate was then incubated overnight at 37 °C under 5% CO 2 atmosphere.…”

Section: Methodsmentioning

confidence: 99%

“…Bacteriocins without the signal peptide have been reported and called leaderless bacteriocins. Among these bacteriocins, the enterocin DD14 (Ent DD14), which is produced by Enterococcus faecalis 14 strain isolated from newborn’s meconium, exerts a wide spectrum of antibacterial activities, specially in combination with other molecules including alginate nanoparticules and antibiotics [ 11 – 14 ]. Interestingly, a recent study by Pérez-Ramos et al [ 15 ] showed that EntDD14 requires for its transport and consequently externalization an ABC transport system and noteworthy a transmembrane canal composed of two pleckstrin-homology domain-containing proteins, named DdE and DdF, in a stoichiometric ratio that remains to be determined.…”

Section: Introductionmentioning

confidence: 99%

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Anti-adhesion and Anti-inflammatory Potential of the Leaderless Class IIb Bacteriocin Enterocin DD14

Teiar

Pérez-Ramos

Zgheib

et al. 2022

Probiotics & Antimicro. Prot.

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In this study, we investigate the interactions between the leaderless class IIb bacteriocin, enterocin DD14 (EntDD14), or the methicillin or the combination of these antibacterials, and two methicillin-resistant Staphylococcus aureus strains (MRSA-S1 and USA 300) which are respectively a clinical strain and a reference strain. The results obtained showed that EntDD14 alone or in combination with the antibiotic could significantly prevent the adhesion of these pathogenic bacteria to human cells. On the other hand, we investigated the anti-inflammatory effect of EntDD14 on the secretion of pro-inflammatory interleukins, including IL-6 and IL-8. The results show that EntDD14 is able to decrease significantly the secretion of both interleukins on Caco-2 cells following their treatments with lipopolysaccharides. These novel data provide insightful informations to support applications of bacteriocins as therapeutic agents capable as well to defeat pathogenic bacteria and concomitantly limit their inflammatory reactions.

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“…This safe, biodegradable, and economically-affordable polymer has been found to be a mucoadhesive and non-immunogenic substance [ 19 , 20 , 21 , 22 , 23 ]. Of note, the optimal loading of proteinaceous AMPs such as bacteriocins on Alg NPs has resulted in a formulation with improved antibacterial activity compared to the use of bacteriocin alone [ 24 , 25 ]. Here, we show that the optimal loading of DRS-B2, which is a member of the dermaseptin family, on Alg NPs also results in a more active formulation compared to DRS-B2 alone when assessed against Escherichia coli, including strains displaying a resistant phenotype to colistin.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Antibacterial Activity of Dermaseptin through Its Immobilization on Alginate Nanoparticles—Effects of Menthol and Lactic Acid on Its Potentialization

et al. 2022

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Dermaseptin B2 (DRS-B2) is an antimicrobial peptide secreted by Phyllomedusa bicolor, which is an Amazonian tree frog. Here, we show that the adsorption of DRS-B2 on alginate nanoparticles (Alg NPs) results in a formulation (Alg NPs + DRS-B2) with a remarkable antibacterial activity against Escherichia coli ATCC 8739 and E. coli 184 strains, which are sensitive and resistant, respectively, to colistin. The antibacterial activity, obtained with this new formulation, is higher than that obtained with DRS-B2 alone. Of note, the addition of lactic acid or menthol to this new formulation augments its antibacterial activity against the aforementioned Gram-negative bacilli. The safety of DRS-B2, and also that of the new formulation supplemented or not with a small molecule such as lactic acid or menthol has been proven on the human erythrocytes and the eukaryotic cell line types HT29 (human) and IPEC-1 (animal). Similarly, their stability was determined under the conditions mimicking the gastrointestinal tract with different conditions: pH, temperature, and the presence of digestive enzymes. Based on all the obtained data, we assume that these new formulations are promising and could be suggested, after in vivo approval and completing regulation aspects, as alternatives to antibiotics to fight infections caused by Gram-negative bacilli such as E. coli.

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Alginate Nanoparticles Enhance Anti-Clostridium perfringens Activity of the Leaderless Two-Peptide Enterocin DD14 and Affect Expression of Some Virulence Factors

Cited by 12 publications

References 58 publications

Lacticaseicin 30 and Colistin as a Promising Antibiotic Formulation against Gram-Negative β-Lactamase-Producing Strains and Colistin-Resistant Strains

Lacticaseicin 30 and Colistin as a Promising Antibiotic Formulation against Gram-Negative β-Lactamase-Producing Strains and Colistin-Resistant Strains

Anti-adhesion and Anti-inflammatory Potential of the Leaderless Class IIb Bacteriocin Enterocin DD14

Enhanced Antibacterial Activity of Dermaseptin through Its Immobilization on Alginate Nanoparticles—Effects of Menthol and Lactic Acid on Its Potentialization

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