Alginate Nanoparticles as a Promising Adjuvant and Vaccine Delivery System

Sarei, F; Dounighi, Naser Mohammadpour; Zolfagharian, Hossein; Khaki, Pejvak; Bidhendi, Soheila Moradi

doi:10.4103/0250-474x.119829

Cited by 111 publications

(21 citation statements)

References 38 publications

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“…This micro-environment is thought to tip responses of oral vaccines towards T H 2 [4] . Furthermore, the main mechanism of action of alginate encapsulated antigens has been proposed to be biased towards T H 2 responses [15] – [18] . These findings are consistent with reports of others using different antigens [19] , [20] and suggest that oral boosting with alginate encapsulated antigens holds promise as a means of augmenting immune responses against IPN.…”

Section: Discussionmentioning

confidence: 99%

Augmentation of the Antibody Response of Atlantic Salmon by Oral Administration of Alginate-Encapsulated IPNV Antigens

et al. 2014

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The objective of the present study was to assess the effect of alginate-encapsulated infectious pancreatic necrosis virus antigens in inducing the immune response of Atlantic salmon as booster vaccines. One year after intraperitoneal injection with an oil-adjuvanted vaccine, post-smolts were orally boosted either by 1) alginate-encapsulated IPNV antigens (ENCAP); 2) soluble antigens (UNENCAP) or 3) untreated feed (control). This was done twice, seven weeks apart. Sampling was done twice, firstly at 7 weeks post 1st oral boost and the 2nd, at 4 weeks after the 2nd oral boost. Samples included serum, head kidney, spleen and hindgut. Serum antibodies were analyzed by ELISA while tissues were used to assess the expression of IgM, IgT, CD4, GATA3, FOXP3, TGF-β and IL-10 genes by quantitative PCR. Compared to controls, fish fed with ENCAP had a significant increase (p<0.04) in serum antibodies following the 1st boost but not after the 2nd boost. This coincided with significant up-regulation of CD4 and GATA3 genes. In contrast, serum antibodies in the UNENCAP group decreased both after the 1st and 2nd oral boosts. This was associated with significant up-regulation of FOXP3, TGF-β and IL-10 genes. The expression of IgT was not induced in the hindgut after the 1st oral boost but was significantly up-regulated following the 2nd one. CD4 and GATA3 mRNA expressions exhibited a similar pattern to IgT in the hindgut. IgM mRNA expression on the other hand was not differentially regulated at any of the times examined. Our findings suggest that 1) Parenteral prime with oil-adjuvanted vaccines followed by oral boost with ENCAP results in augmentation of the systemic immune response; 2) Symmetrical prime and boost (mucosal) with ENCAP results in augmentation of mucosal immune response and 3) Symmetrical priming and boosting (mucosal) with soluble antigens results in the induction of systemic immune tolerance.

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Section: Discussionmentioning

confidence: 99%

Augmentation of the Antibody Response of Atlantic Salmon by Oral Administration of Alginate-Encapsulated IPNV Antigens

et al. 2014

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“…Additionally, an important feature of CaCl 2 cross-linking is that the process can be conducted in an aqueous environment at a low temperature, and that both macromolecular and low molecular weight therapeutic agents can be encapsulated [ 12 , 13 , 14 , 15 ]. Cross-linked ALG is also utilized in biomedicine—in cell encapsulation or tissue engineering [ 16 , 17 , 18 ]. The commonly used and well-described method to obtain ALG microparticles cross-linked by CaCl 2 is the internal gelation by emulsification method [ 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Calcium Chloride Modified Alginate Microparticles Formulated by the Spray Drying Process: A Strategy to Prolong the Release of Freely Soluble Drugs

et al. 2018

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Alginate (ALG) cross-linking by CaCl2 is a promising strategy to obtain modified-release drug delivery systems with mucoadhesive properties. However, current technologies to produce CaCl2 cross-linked alginate microparticles possess major disadvantages, such as a poor encapsulation efficiency of water-soluble drugs and a difficulty in controlling the process. Hence, this study presents a novel method that streamlines microparticle production by spray drying; a rapid, continuous, reproducible, and scalable technique enabling obtainment of a product with low moisture content, high drug loading, and a high production yield. To model a freely water-soluble drug, metformin hydrochloride (MF) was selected. It was observed that MF was successfully encapsulated in alginate microparticles cross-linked by CaCl2 using a one-step drying process. Modification of ALG provided drug release prolongation—particles obtained from 2% ALG cross-linked by 0.1% CaCl2 with a prolonged MF rate of dissolution of up to 12 h. Cross-linking of the ALG microparticles structure by CaCl2 decreased the swelling ratio and improved the mucoadhesive properties which were evaluated using porcine stomach mucosa.

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“…Other factors, such as pH, temperature and stirring speed, may play major roles in controlling the size of alginate nanoparticles [ 130 ]. Of critical importance is the fact that alginate-based nanoparticles avoid aggregation during blood circulation by reducing the interaction with serum proteins and usually do not concentrate in internal organs while they deliver drugs or proteins [ 131 ].…”

Section: Applications Of Alginate In the Medical Fieldmentioning

confidence: 99%

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

et al. 2020

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Initially used extensively as an additive and ingredient in the food industry, alginate has become an important compound for a wide range of industries and applications, such as the medical, pharmaceutical and cosmetics sectors. In the food industry, alginate has been used to coat fruits and vegetables, as a microbial and viral protection product, and as a gelling, thickening, stabilizing or emulsifying agent. Its biocompatibility, biodegradability, nontoxicity and the possibility of it being used in quantum satis doses prompted scientists to explore new properties for alginate usage. Thus, the use of alginate has been expanded so as to be directed towards the pharmaceutical and biomedical industries, where studies have shown that it can be used successfully as biomaterial for wound, hydrogel, and aerogel dressings, among others. Furthermore, the ability to encapsulate natural substances has led to the possibility of using alginate as a drug coating and drug delivery agent, including the encapsulation of probiotics. This is important considering the fact that, until recently, encapsulation and coating agents used in the pharmaceutical industry were limited to the use of lactose, a potentially allergenic agent or gelatin. Obtained at a relatively low cost from marine brown algae, this hydrocolloid can also be used as a potential tool in the management of diabetes, not only as an insulin delivery agent but also due to its ability to improve insulin resistance, attenuate chronic inflammation and decrease oxidative stress. In addition, alginate has been recognized as a potential weight loss treatment, as alginate supplementation has been used as an adjunct treatment to energy restriction, to enhance satiety and improve weight loss in obese individuals. Thus, alginate holds the promise of an effective product used in the food industry as well as in the management of metabolic disorders such as diabetes and obesity. This review highlights recent research advances on the characteristics of alginate and brings to the forefront the beneficial aspects of using alginate, from the food industry to the biomedical field.

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Alginate Nanoparticles as a Promising Adjuvant and Vaccine Delivery System

Cited by 111 publications

References 38 publications

Augmentation of the Antibody Response of Atlantic Salmon by Oral Administration of Alginate-Encapsulated IPNV Antigens

Augmentation of the Antibody Response of Atlantic Salmon by Oral Administration of Alginate-Encapsulated IPNV Antigens

Calcium Chloride Modified Alginate Microparticles Formulated by the Spray Drying Process: A Strategy to Prolong the Release of Freely Soluble Drugs

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

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