Alginate Microencapsulation Technology for the Percutaneous Delivery of Adipose-Derived Stem Cells

Moyer, Hunter R.; Kinney, Ramsey C.; Singh, Kimberly A.; Williams, Joseph K.; Schwartz, Zvi; Boyan, Barbara D.

doi:10.1097/sap.0b013e3181d37713

Cited by 54 publications

(32 citation statements)

References 20 publications

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“…Cells producing the anti‑angiogenic protein endostatin have been encapsulated in ultrapure alginate and implanted into the brains of dogs being treated for spontaneous brain cancer [84,85,86]. Many other cell types have been immobilized in alginate such as adipose-derived stem cells [87,88], mesenchymal stem cells [89,90], and chondrocytes [91,92]. …”

Section: 3d Cell Culturementioning

confidence: 99%

3D Cell Culture in Alginate Hydrogels

2015

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This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of alginate used, its concentration, the choice of gelation technique (ionic or covalent), and divalent cation chosen as the gel inducing ion. The use of peptide-coupled alginate can control cell–matrix interactions. Gelation of alginate with concomitant immobilization of cells can take various forms. Droplets or beads have been utilized since the 1980s for immobilizing cells. Newer matrices such as macroporous scaffolds are now entering the 3D cell culture product market. Finally, delayed gelling, injectable, alginate systems show utility in the translation of in vitro cell culture to in vivo tissue engineering applications. Alginate has a history and a future in 3D cell culture. Historically, cells were encapsulated in alginate droplets cross-linked with calcium for the development of artificial organs. Now, several commercial products based on alginate are being used as 3D cell culture systems that also demonstrate the possibility of replacing or regenerating tissue.

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Section: 3d Cell Culturementioning

confidence: 99%

3D Cell Culture in Alginate Hydrogels

2015

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“…The latter was recently confirmed using a human whole blood model to evaluate the inflammatory properties of several microbeads and microcapsules [5,6]. As evident from recent publications, on the one hand research continues with simple calcium and barium alginate microbeads [7–13], while on the other hand poly(L-lysine) has been replaced by poly(L-ornithine) [14] or chitosan [15–18], or alginate has been combined with other compounds such as carrageenan [19] or synthetic methacrylate-based polymers [20–22]. However, using Na-alg in combination with polycations, the chance of success in terms of biocompatibility is uncertain [23].…”

Section: Introductionmentioning

confidence: 94%

Alginate-Poly(ethylene glycol) Hybrid Microspheres for Primary Cell Microencapsulation

et al. 2014

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The progress of medical therapies, which rely on the transplantation of microencapsulated living cells, depends on the quality of the encapsulating material. Such material has to be biocompatible, and the microencapsulation process must be simple and not harm the cells. Alginate-poly(ethylene glycol) hybrid microspheres (alg-PEG-M) were produced by combining ionotropic gelation of sodium alginate (Na-alg) using calcium ions with covalent crosslinking of vinyl sulfone-terminated multi-arm poly(ethylene glycol) (PEG-VS). In a one-step microsphere formation process, fast ionotropic gelation yields spherical calcium alginate gel beads, which serve as a matrix for simultaneously but slowly occurring covalent cross-linking of the PEG-VS molecules. The feasibility of cell microencapsulation was studied using primary human foreskin fibroblasts (EDX cells) as a model. The use of cell culture media as polymer solvent, gelation bath, and storage medium did not negatively affect the alg-PEG-M properties. Microencapsulated EDX cells maintained their viability and proliferated. This study demonstrates the feasibility of primary cell microencapsulation within the novel microsphere type alg-PEG-M, serves as reference for future therapy development, and confirms the suitability of EDX cells as control model.

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“…The microbead fabrication method prevents alginate calcification and a hyaluronic acid delivery vehicle was developed to ensure that the beads did not aggregate during percutaneous injection. The result is a novel cell delivery system that enables large number of cells to be injected into the wound site in a controlled manner, avoiding cell apoptosis due to poor diffusion of nutrients, and using the cells as living biological factories for production of continuous, controlled release of wound healing factors [57][58][59]. Currently, this technology is being developed to address cartilage regeneration strategies for chondral defects and has been licensed for product and commercialization development.…”

Section: Examples Of Tatrc-sponsored Nanotechnology and Biomaterimentioning

confidence: 99%

Promoting Innovation and Convergence in Military Medicine: Technology-Inspired Problem Solving

Grundfest

Lai

Friedl

2012

IEEE Circuits Syst. Mag.

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Large organizations develop layers and rules for members to operate within accepted processes and conventions whereas innovation tends to occur in a less constrained, less conventional, and less risk averse environment. This basic cultural difference creates a need for protected semi-autonomous centers that cultivate great ideas, providing freedom to explore new concepts and harbor the zealots to champion them past institutional barriers to change. The management objective at the Telemedicine and Advanced Technology Research Center (TATRC) is to advocate and accelerate technology development and ensure benefi cial implementation in the shortest possible time. TATRC accomplishes this objective through integrating multidisciplinary teams that combine engineering technology and physical sciences with both basic and applied clinical biosciences to solve medical problems. This convergence in medical research complements Department of Defense (DoD) investments in long term basic research and large investments in high risk problem solving. TATRC successes in this "technology push to satisfy clinical need" began with radiograph digitization standards and has continued to spin out medical systems and program initiatives to new DoD core programs in rehabilitative medicine (e.g., regenerative medicine, advanced prosthetics, vision research, and integrative pain management), medical modeling and simulation, and current combat zone telemedicine applications. TATRC "technology scouts" look for transformational approaches across traditional boundaries and provide active assistance to build new capabilities and to successfully complete projects through commercialization and DoD implementation. Many near term problems can be addressed by mature technologies in medical robotics, synthetic biology, tissue engineering, nano-and biomaterials science, medical imaging, and neuroengineering. Everyday technologies such as smartphones can be immediately harnessed for better access to medical care, improved safety and efficiency in medicine, technology management and ultimately reduced medical costs. The end result of this culture of convergence can be transformational, calling for disruptive change in technology and capability as exemplifi ed by telemedicine and m-Health, fostered through the unique TATRC research management model.

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Alginate Microencapsulation Technology for the Percutaneous Delivery of Adipose-Derived Stem Cells

Abstract: ADSCs can be readily cultured, encapsulated, and injected in alginate microspheres. Stem cells suspended in alginate microspheres survive in vivo and are seen to replicate in vitro.

Cited by 54 publications

References 20 publications

3D Cell Culture in Alginate Hydrogels

3D Cell Culture in Alginate Hydrogels

Alginate-Poly(ethylene glycol) Hybrid Microspheres for Primary Cell Microencapsulation

Promoting Innovation and Convergence in Military Medicine: Technology-Inspired Problem Solving

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