Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application

Karimi, Sarah; Bagher, Zohreh; Najmoddin, Najmeh; Simorgh, Sara; Pezeshki‐Modaress, Mohamad

doi:10.1016/j.ijbiomac.2020.11.199

Cited by 60 publications

(41 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effect of concentration on the storage (G′) and loss modulus (G″) of collagen hydrogels was evaluated by rheometric mechanical spectroscopy (RMS) tests using an MCR300 rheometer (Anton Paar, Graz, Austria) equipped with a cone-plate geometry [ 11 ]. For sample preparation, the hydrogels were poured into a mold and then placed on the plate of the rheometer; and before beginning the procedure, surface water was carefully removed with tissue paper.…”

Section: Methodsmentioning

confidence: 99%

“…The viability of human OE-MSCs was quantified using a live/dead viability package (ThermoFisher, Berlin, Germany), following the manufacturer’s staining protocol [ 11 ]. Briefly, after stem cell encapsulation in collagen hydrogels on days 1, 7, and 14, the samples were dyed for 10 min after the culture medium was extracted.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, the different type of stem cell has been examined in the tissue engineering field and can be isolated from adult and embryonic tissues [ 10 ]. Immunomodulatory effects, releasing paracrine molecules, and remarkable angiogenic capacity are known as the leading mechanisms for tissue regeneration [ 11 ]. Various mesenchymal stem cells (MSC) sources, such as dental pulp MSCs, human amniotic MSCs, placental-derived MSCs, and mesenchymal cell derived from bone marrow and adipose tissue, have been identified for bone regeneration [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering

et al. 2021

Self Cite

View full text Add to dashboard Cite

For bone tissue engineering, stem cell-based therapy has become a promising option. Recently, cell transplantation supported by polymeric carriers has been increasingly evaluated. Herein, we encapsulated human olfactory ectomesenchymal stem cells (OE-MSC) in the collagen hydrogel system, and their osteogenic potential was assessed in vitro and in vivo conditions. Collagen type I was composed of four different concentrations of (4 mg/mL, 5 mg/mL, 6 mg/mL, 7 mg/mL). SDS-Page, FTIR, rheologic test, resazurin assay, live/dead assay, and SEM were used to characterize collagen hydrogels. OE-MSCs encapsulated in the optimum concentration of collagen hydrogel and transplanted in rat calvarial defects. The tissue samples were harvested after 4- and 8-weeks post-transplantation and assessed by optical imaging, micro CT, and H&E staining methods. The highest porosity and biocompatibility were confirmed in all scaffolds. The collagen hydrogel with 7 mg/mL concentration was presented as optimal mechanical properties close to the naïve bone. Furthermore, the same concentration illustrated high osteogenic differentiation confirmed by real-time PCR and alizarin red S methods. Bone healing has significantly occurred in defects treated with OE-MSCs encapsulated hydrogels in vivo. As a result, OE-MSCs with suitable carriers could be used as an appropriate cell source to address clinical bone complications.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Finally, the use of scaffolds made of various nanomaterials can serve as structural support, induce or mimic the formation of an ECM, inhibit glial differentiation, promote neuronal growth and control hemostasis. In this context, IONPs can be used as part of the biocomposite for diagnostic purposes, to magnetically induce a specific surface topology or neurite outgrowth or to further improve cellular differentiation [ 416 , 417 , 418 , 419 , 420 ].…”

Section: Pns and Cns Regenerationmentioning

confidence: 99%

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

2021

View full text Add to dashboard Cite

In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.

show abstract

“…A number of other studied have also developed MNP‐based scaffolds for tissue engineering applications 246,258–260,264,265 . Application of magnetic field in responsive scaffolds have shown promising results for nerve regeneration 249 . The substantial effect of applied IONPs in nerve scaffolds was seen in terms of the axonal extension, increase in the neurite outgrowth, and activation of the signaling pathways of neuronal differentiation via direct interaction of SPIONs with proteins 248,249 .…”

Section: Applicationsmentioning

confidence: 99%

Progress and prospects of magnetic iron oxide nanoparticles in biomedical applications: A review

Elahi

Rizwan

2021

Artificial Organs

View full text Add to dashboard Cite

Nanoscience has been considered as one of the most substantial research in modern science. The utilization of nanoparticle (NP) materials provides numerous advantages in biomedical applications due to their unique properties. Among various types of nanoparticles, the magnetic nanoparticles (MNPs) of iron oxide possess intrinsic features, which have been efficiently exploited for biomedical purposes including drug delivery, magnetic resonance imaging, Magnetic‐activated cell sorting, nanobiosensors, hyperthermia, and tissue engineering and regenerative medicine. The size and shape of nanostructures are the main factors affecting the physicochemical features of superparamagnetic iron oxide nanoparticles, which play an important role in the improvement of MNP properties, and can be controlled by appropriate synthesis strategies. On the other hand, the proper modification and functionalization of the surface of iron oxide nanoparticles have significant effects on the improvement of physicochemical and mechanical features, biocompatibility, stability, and surface activity of MNPs. This review focuses on popular methods of fabrication, beneficial surface coatings with regard to the main required features for their biomedical use, as well as new applications.

show abstract

Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application

Cited by 60 publications

References 48 publications

Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering

Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

Progress and prospects of magnetic iron oxide nanoparticles in biomedical applications: A review

Contact Info

Product

Resources

About