Alginate Lyase of a Novel Algae Fermentation Strain

Abstract

A novel algae fermentation strain was obtained in our previous work. This strain can produce alginate lyase and alcohol dehydrogenase used for the ethanol fermentation from algae. This research investigated the fermentation, separation and purification of alginate lyase, and the molecular weight of alginate lyase was determined. The optimum conditions for enzyme fermentation were as follows: fermentation medium with 20 g L–1 alginate, initial pH 6.0, and temperature 35 °C. The flasks were cultured in a shaking incubator at 120 rpm for 96 h. The enzyme was purified using the method of salting out, dialysis, and gel chromatography. After purification, the SDS-PAGE method was used to determine the molecular weight of the protein. The molecular weight of alginate lyase was 30–35 KDa. This research contributes to algae biodegradation and fuels production from algae.

Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials

“…Such requirements are fulfilled by alginates, marine-originated [24], biodegradable [25][26][27][28][29] biopolymers applied as a basis for drug delivery, wound dressings, and tissue engineering [30][31][32][33][34].…”

“…Such requirements are fulfilled by alginates, marine-originated [ 24 ], biodegradable [ 25 , 26 , 27 , 28 , 29 ] biopolymers applied as a basis for drug delivery, wound dressings, and tissue engineering [ 30 , 31 , 32 , 33 , 34 ]. The strong affinity of alginates to metal cations allows for their application as antibacterial hybrids (e.g., [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ]), and also for the removal of heavy metal salts, e.g., copper, lead, and mercury (e.g., [ 45 , 46 , 47 , 48 , 49 , 50 ]).…”

Ethanol Production from Colpomenia sinuosa by an Alginate Fermentation Strain Meyerozyma guilliermondii

“…All biochemical reagents were purchased from Bio-Technology Factory (Beijing, China). The alginate fermentation yeast Meyerozyma guilliermondii was obtained from our previous study [ 19 ]. The yeast was stored at 4 °C.…”

“…The mass of ethanol produced was the volume of distillate (L) multiplied by the ethanol concentration (g L −1 ). The enzyme activity of alginate lyase was measured according to the reference [ 19 ]. The components of Colpomenia sinuosa was measured by the following methods according to the reference [ 20 ].…”

“…was EF375700). Meyerozyma guilliermondii can produce alcohol dehydrogenase (ADH) and alginate lyase to realize the alginate fermentation [ 19 ]. It can also utilize other sugars such as mannitol and glucose.…”