Alginate enhances Toll-like receptor 4-mediated phagocytosis by murine RAW264.7 macrophages

Bi, Decheng; Zhou, Rui; Cai, Nan; Lai, Qiuxian; Han, Qingguo; Peng, Yanwen; Jiang, Zedong; Tang, Zhishu; Lu, Jun; Bao, Wei-Yang; Xu, Hong; Xu, Xu

doi:10.1016/j.ijbiomac.2017.07.129

Cited by 47 publications

(28 citation statements)

References 33 publications

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“…TLR4 is a very important class of membrane receptors that are expressed on the surfaces of macrophages and mediate the activation of macrophages by converting extracellular signals (61–64). The binding of TLR4 to the ligand results in the formation of a complex between the TLR cytoplasmic domain, the adaptor protein myeloid differentiation primary response gene 88 (MyD88), and the interleukin-1 receptor-associated kinase (IRAK), which in turn activates tumor necrosis factor receptor-associated factor 6 (TRAF6) and finally initiates the mitogen-activated protein kinase (MAPK) signaling pathway (65–67).…”

Section: Mechanism Of Immunoregulation By Plant Polysaccharidesmentioning

confidence: 99%

Advances in Research on Immunoregulation of Macrophages by Plant Polysaccharides

2019

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Polysaccharides are among the most important members of the biopolymer family. They are natural macromolecules composed of monosaccharides. To date, more than 300 kinds of natural polysaccharide compounds have been identified. They are present in plants, animals, and microorganisms, and they engage in a variety of physiological functions. In the 1950s, due to the discovery of their immunoregulatory and anti-tumor activities, polysaccharides became a popular topic of research in pharmacology, especially in immunopharmacology. Plants are an important source of natural polysaccharides. Pharmacological and clinical studies have shown that plant polysaccharides have many functions, such as immune regulation, anti-tumor activity, anti-inflammatory activity, anti-viral functions, anti-radiation functions, and a hypoglycaemic effect. The immunomodulatory effects of plant polysaccharides have received much attention. Polysaccharides with these effects are also referred to as biological response modifiers (BRMs), and research on them is one of the most active areas of polysaccharide research. Thus, we summarize immunomodulatory effects of botanical polysaccharides isolated from different species of plants on the macrophage. The primary effect of botanical polysaccharides is to enhance and/or activate macrophage immune responses, including increasing reactive oxygen species (ROS) production, and enhancing secretion of cytokines and chemokines. Therefore, it is believed that botanical polysaccharides have significant therapeutic potential, and represent a new method for discovery and development of novel immunomodulatory medicine.

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Section: Mechanism Of Immunoregulation By Plant Polysaccharidesmentioning

confidence: 99%

Advances in Research on Immunoregulation of Macrophages by Plant Polysaccharides

2019

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“…Macrophages serve important roles in innate immunity, displaying a high degree of plasticity and polarize into two main subtypes: Classically activated M1 macrophages and alternatively activated M2 macrophages (13,14). Previous studies have reported that M1 macrophages can be activated by interferon-γ and toll-like receptors, which display a proinflammatory phenotype by producing proinflammatory cytokines, including IL-1, IL-6, TNF-α and IL-12 (15,16). Macrophages can also be polarized into an anti-inflammatory phenotype, known as M2 macrophages, which promote T helper cell 2 responses and contribute to tissue repair and healing (17,18).…”

Section: Introductionmentioning

confidence: 99%

miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis

Cui

Wang

2020

Exp Ther Med

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Osteoarthritis (OA) is one of the most prevalent degenerative joint diseases that affects millions of individuals worldwide. During OA, proinflammatory factors (including IL-1, IL-6, IL-17 and TNF-α) are released from chondrocytes and proliferating synoviocytes potentiate the proinflammatory microenvironment of the synovial fluid (SF). The altered SF microenvironment affects the infiltration, polarization and apoptosis of macrophages, though the underlying mechanisms are not completely understood. In the present study, the hypothesis that the knee synovial fluid of patients with knee osteoarthritis (KOA SF) promotes the polarization of peripheral blood mononuclear cell (PBMC)-derived M1 macrophages and inhibits PBMC-derived macrophage apoptosis was investigated. KOA SF increased PBMC-derived macrophage M1 polarization via the microRNA (miR)-155-5p/suppressor of cytokine signaling 1 signaling pathway. Caspase-3 (CASP3) was identified as a novel target of miR-155-5p, where KOA SF inhibited macrophage apoptosis via the miR-155-5p/CASP3 signaling pathway. The results suggested that the proinflammatory environment of KOA SF promoted macrophage M1 polarization and reduced macrophage apoptosis via miR-155-5p. The results provided a potential explanation for the increased number of M1 macrophages observed in KOA SF during OA. In addition, the present study suggested that miR-155-5p may serve as a potential therapeutic target for KOA.

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“…Macrophages initiate phagocytosis by identifying molecular patterns associated with the pathogen surface through pattern recognition receptors, which include lectins, mannose receptor, Toll-like receptors (TLRs), and Fcγ receptor. TLR4, a member of the TLR family, plays a critical role in pathogen detection and triggering immune responses by initiating signal transduction [40]. Nuclear factor-κB (NF-κB) protein complex and mitogen-activated protein kinase are key elements related to proinflammatory signaling, and NF-κB is an evolutionarily conserved transcription factor that modulates the expression of various genes involved in immune responses [40].…”

Section: Dna Damage Repair Foci Increased After Exposure To Binpsmentioning

confidence: 99%

“…TLR4, a member of the TLR family, plays a critical role in pathogen detection and triggering immune responses by initiating signal transduction [40]. Nuclear factor-κB (NF-κB) protein complex and mitogen-activated protein kinase are key elements related to proinflammatory signaling, and NF-κB is an evolutionarily conserved transcription factor that modulates the expression of various genes involved in immune responses [40]. As both signaling pathways are considered to be involved in phagocytosis, BiNPs could also be interfering with these pathways in RAW 264.7 cells.…”

Section: Dna Damage Repair Foci Increased After Exposure To Binpsmentioning

confidence: 99%

Cytotoxicity of bismuth nanoparticles in the murine macrophage cell line RAW 264.7

Luz

Machado

Bezerra

et al. 2020

J Mater Sci: Mater Med

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A promising use of bismuth nanoparticles (BiNPs) for different biomedical applications leads to a search for the elucidation of their toxicity mechanisms, since toxicity studies are still at early stage. In the current study, cytotoxic effects of BiNPs produced by laser ablation in solution (LASiS) was investigated in the murine macrophage line RAW 264.7. The cells were exposed to 0.01-50 µg ml −1 of BiNPs for 24 and 48 h and then cytotoxicity assays were performed. Decrease of MTT conversion to formazan and of cell attachment were observed with no effects on cell proliferation. No loss of membrane integrity or significant changes of ROS and RNS levels were observed in exposed cells. Foremost, increased phagocytic activity and DNA repair foci occurred for cells exposed to BiNPs. These effects are important findings that must be considered in the case of biomedical application of BiNPs, since inappropriate macrophages activation and inactivation may lead to immunotoxicity.

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Alginate enhances Toll-like receptor 4-mediated phagocytosis by murine RAW264.7 macrophages

Cited by 47 publications

References 33 publications

Advances in Research on Immunoregulation of Macrophages by Plant Polysaccharides

Advances in Research on Immunoregulation of Macrophages by Plant Polysaccharides

miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis

Cytotoxicity of bismuth nanoparticles in the murine macrophage cell line RAW 264.7

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