Alginate encapsulation technology supports embryonic stem cells differentiation into insulin-producing cells

Wang, Nan; Adams, Gary G.; Buttery, Lee D.K.; Falcone, Franco H.; Stolnik, Snow

doi:10.1016/j.jbiotec.2009.08.008

Cited by 126 publications

(100 citation statements)

References 29 publications

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“…The immobilization of cells in particles is widely discussed in tissue engineering; the possibility to encapsulate non-human cells reduces the host's immune system response, facilitating transplantation as an alternative to the limited donor tissues available [1]. The technique of cell encapsulation is being used for the production of "Bio artificial Pancreas" [2], immunobarrier for islet cells transplantation [2][3][4], and the encapsulation of stem cells [5,6]. Encapsulation of living cells was also suggested for the production of chemicals such as alcohol, organic acids, steroids, antibiotics, vaccines [7].…”

Section: Introductionmentioning

confidence: 99%

“…Eudragit coated microparticles were produced for the colonic release of therapeutics [16,18] or proteins [19], but it has not yet been applied for the coating of encapsulated cells. A variety of materials have been tested for the encapsulation of living cells; alginate [4,6,8,12,15,[20][21][22][23] is the most common and versatile, chitosan [9,23], gelatin [22,24], cellulose [23,25], agarose [23,26], dextran [1], carrageenan [9,12,27], poly(lactide-co-glycolide) (PLGA) [1,23], Poly (Ethylene Glycol) [23,28] all have been used individually, and in blends [1,27]. Chitosan is a proven biocompatible natural polymer produced from natural sources (crustacean shells, fungi, and insects), which has been widely used for cell encapsulation and other pharmaceutical purposes [23,29].…”

Section: Introductionmentioning

confidence: 99%

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Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Morelli

Holdich

Dragosavac

2017

Journal of Membrane Science

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Citation: MORELLI, S., HOLDICH, R. and DRAGOSAVAC, M., 2017. Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification. Journal of Membrane Science, 524, Additional Information:• This paper was accepted for publication in the journal Journal of This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. conditions. The liquid drops were loaded with increasing amount of yeast (3.14 x 10 7 to 3.14 x 10 8 cells/ mL). The stability and uniformity of the emulsions was independent of the cell concentration. PTFE coated hydrophobic membrane produced smaller W/O drops compared to FAS coated membranes. The liquid polymeric droplets were solidified in solid particles using thermal gelation and/or ionic crosslinking, obtaining yeast encapsulated particles sized ~100 µm.The pH sensitive polymer, Eudragit S100, was used as a coating to create gastro resistant particles suitable for intestinal-colonic targeted release. Viability of the released yeast cells was demonstrated using fluorescence probes and checking cell glucose metabolism with time. AbbreviationsW/O emulsion, water in oil

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Morelli

Holdich

Dragosavac

2017

Journal of Membrane Science

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“…18 Alginate is a biomaterial, typically derived from seaweed or algae, which is commonly used as a wound dressing in the treatment of pressure ulcers and has a long history of biocompatibility. 1,19,20 The purpose of this report is to demonstrate, through a small three-person case study, the results of using CaCl 2 -activated PRP and PRP containing alginate beads as a delivery vehicle for the sustained release of PRP-derived growth factors and cytokines to stimulate healing in stalled pressure ulcers where conventional treatment methods have failed.…”

Section: Introductionmentioning

confidence: 99%

A case report on the use of sustained release platelet-rich plasma for the treatment of chronic pressure ulcers

Sell

Ericksen

Reis

et al. 2011

The Journal of Spinal Cord Medicine

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Background/objectives: Chronic pressure ulcers affect patient health, emotional state, and quality of life, causing considerable morbidity and mortality in addition to contributing to significant health care costs from lengthy hospitalizations to advanced home care and surgical care costs. The conventional treatment of these wounds can be slow due to their chronic inflammatory state and the senescence of local reparative cells. Platelet-rich plasma (PRP) therapy has been growing as a viable treatment alternative for a number of clinical applications and has potential benefit for use in chronic wounds. The sustained release of large quantities of autologous growth factors, cytokines, and other mediators found in PRP plus the favorable mononuclear cell profile of PRP may help us to stimulate wound healing and resolve chronic inflammation. Methods: Three veterans with spinal cord injury (SCI), presenting with chronic stage IV pressure ulcers, were treated with a sustained release PRP therapy to stimulate wound healing. Results: PRP treatment consistently resulted in the formation of granulation tissue and improved vascularity for each of the three patients treated, while reducing the overall ulcer area and volume. Conclusion: The controlled release of growth factors from PRP demonstrated a positive stimulatory effect on the healing rate of chronic pressure ulcers in individuals with SCI.

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“…Various technologies yield spherical hydrogels with diameters adjustable in the range of a few microns to few millimeters upon extrusion of a sodium alginate (Na-alg) solution through a needle or nozzle into a gelation bath. Such microspheres serve as containers for a multitude of materials including solids, vitamins, hormones and even living cells ( [5][6][7][8]). Our idea was to encapsulate iron particles (CIP, carbonyl iron powder) into Ca-alg and subsequently to incorporate these into epoxy material.…”

Section: Introductionmentioning

confidence: 99%

Alginate Beads and Epoxy Resin Composites as Candidates for Microwave Absorbers

Živković¹,

Wandrey²,

Bogićević³

2012

PIER C

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Abstract-This paper presents a new composite material, which is developed by mixing calcium alginate spheres with commercially available epoxies Stycas 2850 FT (s2850) and Stycast W19 (W19). The resulting composite material is examined in terms of transmission and reflection coefficients in microwave frequencies (26 to 40 GHz, 70 to 110 GHz and 300 to 320 GHz). The study reveals that the new material exhibits reflection coefficients much lower than some commercial CR absorbers from the Eccosorb group. The experimental results justify the use of the new composite material as absorber at microwave frequencies.

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Alginate encapsulation technology supports embryonic stem cells differentiation into insulin-producing cells

Cited by 126 publications

References 29 publications

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

A case report on the use of sustained release platelet-rich plasma for the treatment of chronic pressure ulcers

Alginate Beads and Epoxy Resin Composites as Candidates for Microwave Absorbers

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