Alginate/chitosan microparticles for gastric passage and intestinal release of therapeutic protein nanoparticles

Ling, Kevin; Wu, Huixia; Neish, Andrew S.; Champion, Julie A.

doi:10.1016/j.jconrel.2018.12.017

Cited by 96 publications

(64 citation statements)

References 81 publications

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“…The presence of potassium ions from the STPP appears to be a very important factor in the formation of nanodisk shapes, leading the rapid increase in the agglomeration and particle size produced by a compact membrane of chitosan on the surface of the nanodisks [31,32]. These results of particle size distribution and hydrodynamic size are consistent with the SEM images reported above and are in agreement with the results reported in the literature about the synthesis of chitosan/alginate nanoparticles by ionotropic gelation [6,33].…”

Section: Resultssupporting

confidence: 91%

“…Polysaccharides such as chitosan and alginate are abundant and can be found easily in the environment and possess excellent physicochemical and biological properties such as biocompatibility, biodegradability, and low toxicity, which demonstrate that these biopolymers are suitable for sustained and controlled drug delivery systems [3][4][5]. These biopolymers are catalogued as Generally Regarded as Safe (GRAS) by the Food and Drug Administration (FDA), allowing their use for drug delivery systems [6].…”

Section: Introductionmentioning

confidence: 99%

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Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

Patiño-Ruiz

Marrugo

Reyes

et al. 2020

International Journal of Polymer Science

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We report on the synthesis of chitosan-alginate nanodisks (Cs-Al NDs) using a simple approach consisting of the ionotropic gelation method. Sodium tripolyphosphate (STPP) was used as crosslinking agent to promote the electrostatic interaction between amine groups the chitosan and hydroxyl and carboxyl groups of alginate. Scanning electron microscopy (SEM) images provided direct evidence of the morphology of the nanodisks where agglomeration was observed due to the electrostatic interaction between the functional groups. Furthermore, dynamic light scattering (DLS) showed that the hydrodynamic size of the Cs-Al NDs was 227 nm and 152 nm in pH 1.2 and pH 7.4, respectively, which is in agreement with the information observed in the SEM images. The chemical structure is presented mainly the amine and carboxyl groups due to the presence of chitosan and alginate in the nanodisks, respectively, which allow the electrostatic interaction through N-H linkages. According to the X-ray diffraction, we found that the Cs-Al NDs exhibited the typical structure of chitosan and alginate, which lead the formation of polyelectrolyte complexes. We also evaluated the encapsulation of amoxicillin in the nanodisk, obtaining a loading efficiency of 74.98%, as well as a maximum in vitro release amount of 63.2 and 52.3% at pH 1.2 and 7.4, respectively. Finally, the cytotoxicity effect of the Cs-Al nanodisks was performed in human prostatic epithelial PWR-1E and Caucasian prostate adenocarcinoma PC-3 cell lines, in which the cell viability was above 80% indicating low inhibition and determining the Cs-Al NDs as a promising technology for controlled delivery systems.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

Patiño-Ruiz

Marrugo

Reyes

et al. 2020

International Journal of Polymer Science

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“…Song fabricated magnetic alginate/CHI layer-by-layer nanoparticles to deliver curcumin for treating with breast cancer cells [21]. The biocompatibility and cellular uptake characteristics of alginate-based NPs are of great importance for biomedical applications [22,23]. The effective cellular uptake profile of NPs may influence its performance as a drug or nutrient carrier [24].…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate Nanoparticles

Zhang

Zhao

et al. 2019

Molecules

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Polymeric nanoparticles could offer promising controlled drug delivery. The biocompatibility is of extreme importance for future applications in humans. Self-assembled polymeric nanoparticles based on phenylalanine ethyl ester (PAE)-modified alginate (Alg) had been successfully prepared and characterized in our lab. However, little is known about their interaction with cells and other biological systems. In this study, nanoparticles (NPs) based on PAE-Alg conjugates (PEA-NPs) with different degree of substitution (DS) were prepared and investigated. Our results showed that PEA-NPs had no effects on the proliferation of the human intestinal epithelial Caco-2 cells at concentrations up to 1000 μg/mL. Furthermore, the in vitro cellular uptake profile of PEA-NPs, concerning several parameters involved in the application of therapeutic or diagnostic NPs, such as NPs concentration, time and temperature, was described. Different NPs have been adopted for cellular uptake studies and the NPs internalized into Caco-2 cells were quantified. Cellular uptake efficiency could reach 60% within 4 h. PEA-NPs also showed greater cell permeability than oleoyl alginate ester nanoparticles (OAE-NPs) previously prepared in our lab. Our studies reveal that NPs based on PEA conjugate are promising nanosystems for cellular delivery.

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“…All these significant inherent properties make chitosan the best option for pharmaceutical and biomedical applications. Chitosan hydrogels have been used widely as gastric drug delivery systems due to its anti‐ulcer and anti‐microbial properties . Chitosan has a semicrystalline structure and several H‐bond‐forming functional groups and correspondingly its cohesive energy density is high .…”

Section: Introductionmentioning

confidence: 99%

“…Chitosan hydrogels have been used widely as gastric drug delivery systems due to its anti-ulcer and anti-microbial properties. [11] Chitosan has a semicrystalline structure and several H-bond-forming functional groups and correspondingly its cohesive energy density is high. [12] Thus solving chitosan in water and usual organic solvents is difficult.…”

Section: Introductionmentioning

confidence: 99%

Tunable Thermo‐ and pH‐Responsive Hydrogels and MWCNTs/Hydrogel Containing 2‐Aminobenzamide Units in Their Crosslink Chains

et al. 2019

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In the present article, a new class of chitosan hydrogels containing 2‐aminobenzamide units in their crosslink chains was prepared by reaction of chitosan with different percentages of bis‐isatoic anhydride as cross‐linker at 70 °C. Then, multi‐walled carbon nanotubes (MWCNTs)/hydrogel nanocomposites were fabricated by crosslinking of chitosan with bis‐isatoic anhydride in the presence of 0.1% and 0.3% (w/w) MWCNTs. Scanning electron microscopy images have verified a 3D porous nanostructure with uniform morphology which MWCNTs were dispersed uniformly on the matrix of hydrogel nanocomposite. The swelling behaviours of the hydrogels and their nanocomposites were investigated by swelling the gels in media of different temperatures and pH. The results indicated the pH‐thermo dual behaviour of hydrogels. Finally, metronidazole was selected as a model drug for the investigation of the drug release properties of the prepared hydrogels. The metronidazole release was affected by physical properties of medium and chemical content of hydrogels.

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Alginate/chitosan microparticles for gastric passage and intestinal release of therapeutic protein nanoparticles

Cited by 96 publications

References 81 publications

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate Nanoparticles

Tunable Thermo‐ and pH‐Responsive Hydrogels and MWCNTs/Hydrogel Containing 2‐Aminobenzamide Units in Their Crosslink Chains

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