Alginate-Chitosan Microencapsulated Cells for Improving CD34+ Progenitor Maintenance and Expansion

Abstract

Protocols for isolation, characterization, and transplantation of hematopoietic stem cells (HSCs) have been well established. However, difficulty in finding human leucocyte antigens (HLA)-matched donors and scarcity of HSCs are still the major obstacles of allogeneic transplantation. In this study, we developed a double-layered microcapsule to deliver paracrine factors from non-matched or low-matched HSCs to other cells. The umbilical cord blood-derived hematopoietic progenitor cells, identified as CD34+ cells, were entrapped in alginate polymer and further protected by chitosan coating. The microcapsules showed no toxicity for surrounding CD34+ cells. When CD34+ cells-loaded microcapsules were co-cultured with bare CD34+ cells that have been collected from unrelated donors, the microcapsules affected surrounding cells and increased the percentage of CD34+ cell population. This study is the first to report the potency of alginate-chitosan microcapsules containing non-HLA-matched cells for improving proliferation and progenitor maintenance of CD34+ cells.

The Effect of Umbilical Cord Blood Serum and Platelet-Rich Plasma Coatings on the Characteristics of Poly(?-caprolactone) Scaffolds for Skin Tissue Engineering Applications

“…Human UCBS was isolated from umbilical cord blood obtained post-C-section surgery using a gradient density centrifugation method (Nurhayati et al, 2021b). The blood was pipetted onto the Ficoll solution, centrifuged at 400 × g for 10 min, and the top layer containing UCBS was carefully transferred to a new tube and filtered with a 0.2 µm membrane filter.…”

Tissue Mechanics and Tissue Engineering

“…Two papers face different applications of tissue regeneration: the skin [1] and the retina [2]. One paper [3] deals with cell encapsulation in biomaterials for protection against immune reactions. Two papers underline that tissue mechanics play a key role in understanding tissue functions, the former focusing on the role of biomechanics related to the prediction of aortic aneurysm rupture [4], whereas the latter on the role of biomechanics for early diagnosis of glaucoma [5].…”

“…Nurhayati et al [3] propose a novel design of a three-dimensional (3D) scaffold for encapsulating CD34+ cells and the idea of co-culturing CD34+ cells from different donors. Microencapsulated CD34+ cells showed no toxicity for surrounding CD34+ cells and improved their proliferation.…”

“…Scaffolds are designed to mimic the native extracellular matrix, thus allowing cell growth and differentiation while avoiding adverse reactions [1,2]. Autologous cells or co-cultures of donor cells either encapsulated or seeded on scaffolds are deeply studied in response to chemical and physical stimuli with an effort to make them survive, grow, and differentiate [3].…”

Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics

“…Emulsification is a technology used to protect valuable therapeutic molecules [1,2], living cells [3], or even secretory tissues/islets [4][5][6] in sealed capsules that can release these contents at controlled rates under the influences of specific conditions. This technology dampens or even prevents rapid degradation [7], facilitates immuno-isolation [8], and inactivates target materials until the product reaches the intended spatial location [1,2].…”