Alginate/Chitosan-Based Hydrogel Film Containing α-Mangostin for Recurrent Aphthous Stomatitis Therapy in Rats

Abstract

Recurrent aphthous stomatitis (RAS) is a prevalent clinical disorder that causes mouth ulcers. Furthermore, corticosteroid treatment has been widely utilized for RAS therapy; however, it has side effects on the oral mucosa that limit its application. This study aimed to develop a novel RAS therapy with the natural ingredient α-mangostin, delivered by alginate and chitosan polymers-based hydrogel film (α-M Alg/Chi-HF). To prepare α-M Alg/Chi-HF, the solvent evaporation and casting methods were used, then characterized by using SEM, FTIR, and XRD. Based on the characterization studies, the α-M in α-M/EtOH Alg/Chi-HF with ethanol (EtOH) was found to be more homogenous compared to α-M in Alg/Chi-HF with distilled water (H2O) as a casting solvent. The in vitro viability study using NIH3T3 cells showed 100% viability of α-M Alg/Chi-HF (EtOH) and Alg/Chi-HF after 24 h incubation, indicating well tolerability of these hydrogel films. Interestingly, the in vivo studies using male white rats (Rattus norvegicus Berkenhout) proved that α-M/EtOH Alg/Chi-HF with a recovery of 81.47 ± 0.09% in seven days significantly more effective RAS therapy compared to control. These results suggest that α-M/EtOH Alg/Chi-HF has the potential as an alternative for RAS therapy.

α-Mangostin hydrogel film with chitosan alginate base for recurrent aphthous stomatitis (RAS) treatment: study protocol for double-blind randomized controlled trial

“…A study demonstrated that an in vivo investigation of αmangostin hydrogel films with a chitosan-alginate base for RAS therapy exhibited a favorable healing response, achieving a healing rate of 93% on the seventh day (Milanda et al, 2022). These promising outcomes have motivated us to conduct a Clinical Trial of α-mangostin Hydrogel Films with a Chitosan-Alginate Base with the aim of evaluating the clinical efficacy and safety in treating patients with RAS.…”

“…In a prior study, the development and assessment of αmangostin hydrogel films with a chitosan-alginate base for in vivo therapy of RAS were conducted using white Wistar rats as test subjects. The results showed a remarkable 93% healing rate within 7 days (Milanda et al, 2022). As a result, further investigations will be carried out to evaluate the clinical efficacy and safety of the chitosan-alginate-based α-mangostin hydrogel film in RAS patients.…”

Chitosan-Coated Bacterial Cellulose (BC)/Hydrolyzed Collagen Films and Their Ascorbic Acid Loading/Releasing Performance: A Utilization of BC Waste from Kombucha Tea Fermentation

“…After that, the films were blotted with filter paper to remove excess water and measured by an UV-Vis spectrophotometer (Labtech, Hopkinton, MA, USA) at λ max = 265 nm. The ascorbic acid loading efficiency, amount of adsorbate, and loading capacity were calculated using the following formula [ 39 ]: where A 0 is the absorbance of the initial solution and A is the absorbance of the tested solution. …”

Alginate and Chitosan-Based Hydrogel Enhance Antibacterial Agent Activity on Topical Application

“…Chitosan is a product resulting from the partial deacetylation of chitin, possessing excellent antibacterial properties, adhesive power, oxygen permeability, non-toxicity, and easy biodegradation, holding great potential for medical applications, such as antibacterial medical dressings. 56 The crucial aspect of chitosan-based hydrogels for use in medical dressings is their host acceptance. Researchers have employed oxidized polysaccharides, like glucomannan, as environmentally friendly bio-crosslinkers.…”

“…The presence of reactive oxygen species (ROS) generated massively around damaged tissues can cause damage to DNA and proteins, inhibiting tissue regeneration. 56 …”