Alginate Biosynthesis and Biotechnological Production

Moradali, M. Fata; Ghods, Shirin; Rehm, Bernd H. A.

doi:10.1007/978-981-10-6910-9_1

Cited by 42 publications

(35 citation statements)

References 115 publications

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“…Next, the authors examined whether there was a functional connection between the biofilms and the adjacent NET layer. Consistently, the NET barrier was absent upon infection with mutant bacteria that were deficient in biofilm formation due to lack of the psl gene cluster that produces the key polysaccharide Psl, indicating that NET formation depended on biofilm formation (Moradali et al, 2017). But even in the absence of NETs and the biofilm, neutrophils were not able to eradicate the infection, and eye pathology persisted despite their ability to engage single bacteria.…”

mentioning

confidence: 91%

“…Pseudomonas aeruginosa is a gram-negative bacterium that has perfected the art of biofilm formation. This microbe causes a variety of opportunistic infections predominately in immune-compromised individuals but is best known for causing chronic respiratory infections in cystic fibrosis (CF) (Moradali et al, 2017). The basis for the specific susceptibility of CF patients to P. aeruginosa infection is poorly understood.…”

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confidence: 99%

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Neutrophils Facing Biofilms: The Battle of the Barriers

Papayannopoulos

2019

Cell Host & Microbe

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confidence: 91%

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confidence: 99%

Neutrophils Facing Biofilms: The Battle of the Barriers

Papayannopoulos

2019

Cell Host & Microbe

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“…Alginate-and mannitol-correlated pathways responsible for their accumulation Moradali et al (2018) reported that F6P is produced via pyruvate metabolism, TCA cycle and gluconeogenesis in bacterial alginate biosynthesis [12]. Our transcriptome analysis identified the importance of glyoxylate cycle in addition to TCA cycle, which might supply GTP for alginate synthesis in brown algae.…”

Section: Content Variations Of Alginate and Mannitolmentioning

confidence: 88%

“…Some studies regarding to variations in alginate and mannitol between months and structures in brown seaweeds have been reported [9][10][11]. To date, the biosynthesis pathways of alginate and mannitol in algae and regulatory mechanism of their contents remain largely unknown [12]. Hence, it is worth investigating the genes involved in alginate and mannitol pathways through transcriptional profiles.…”

Section: Japonica Backgroundmentioning

confidence: 99%

Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis

Shao

Zhang

et al. 2019

Preprint

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Background: Alginate is an important cell wall component and mannitol is a soluble storage carbon substance in brown seaweed Saccharina japonica. Their contents vary with kelp developmental periods and harvesting time. Alginate and mannitol regulatory networks and molecular mechanisms are largely unknown. Results: With WGCNA and trend analysis of 20,940 known genes and 4,264 new genes produced from transcriptome sequencing of 30 kelp samples from different stages and tissues, we deduced that ribosomal proteins, light harvesting complex proteins and imm upregulated 3 gene family are closely associated with the meristematic growth and kelp maturity. Moreover, 134 and 6 genes directly involved in the alginate and mannitol metabolism were identified, respectively. Mannose-6-phosphate isomerase (MPI2), phosphomannomutase (PMM1), GDP-mannose 6-dehydrogenase (GMD3) and mannuronate C5-epimerase (MC5E70 and MC5E122) are closely related with the high content of alginate in the distal blade. Mannitol accumulation in the basal blade might be ascribed to high expression of mannitol-1-phosphate dehydrogenase (M1PDH1) and mannitol-1-phosphatase (M1Pase) (in biosynthesis direction) and low expression of mannitol-2-dehydrogenase (M2DH) and fructokinase (FK) (in degradation direction). Oxidative phosphorylation and photosynthesis provide ATP and NADH for mannitol metabolism whereas glycosylated cycle and tricarboxylic acid (TCA) cycle produce GTP for alginate biosynthesis. RNA/protein synthesis and transportation might affect alginate complex polymerization and secretion processes. Cryptochrome (CRY-DASH), xanthophyll cycle, photosynthesis and carbon fixation influence the production of intermediate metabolite of fructose-6phosphate, contributing to high content of mannitol in the basal blade. Conclusions: The network of co-responsive DNA synthesis, repair and proteolysis are presumed to be involved in alginate polymerization and secretion, while upstream light-responsive reactions are important for mannitol accumulation in meristem of kelp. Our transcriptome analysis provides new insights into the transcriptional regulatory networks underlying the biosynthesis of alginate and mannitol during S. japonica developments.

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“…Alginates are isolated from the cell walls of brown algae (Laminaria digtata/Ascophyllum nodosum), where they are found in the form of sodium, calcium, and magnesium salts of alginic acid [29]; they can also be synthesized by microorganisms [30]. Alginate is a linear, anionic, water-soluble polysaccharide, and its most useful property-the formation of strong gels or polymers with low solubility (Figure 1)-is due to its ability to react with polyvalent metal cations, especially with calcium ions.…”

Section: Sodium Alginate-a Basic Component Of Bio-based Polymer Matermentioning

confidence: 99%

The Use of Edible Films Based on Sodium Alginate in Meat Product Packaging: An Eco-Friendly Alternative to Conventional Plastic Materials

2020

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The amount of plastics used globally today exceeds a million tonnes annually, with an alarming annual growth. The final result is that plastic packaging is thrown into the environment, and the problem of waste is increasing every year. A real alternative is the use bio-based polymer packaging materials. Research carried out in the laboratory context and products tested at the industrial level have confirmed the success of replacing plastic-based packaging with new, edible or completely biodegradable foils. Of the polysaccharides used to obtain edible materials, sodium alginate has the ability to form films with certain specific properties: resistance, gloss, flexibility, water solubility, low permeability to O2 and vapors, and tasteless or odorless. Initially used as coatings for perishable or cut fresh fruits and vegetables, these sodium alginate materials can be applied to a wide range of foods, especially in the meat industry. Used to cover meat products, sodium alginate films prevent mass loss and degradation of color and texture. The addition of essential oils prevents microbial contamination with Escherichia coli, Salmonella enterica, Listeria monocytogenes, or Botrytis cinerea. The obtained results promote the substitution of plastic packaging with natural materials based on biopolymers and, implicitly, of sodium alginate, with or without other natural additions. These natural materials have become the packaging of the future.

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Alginate Biosynthesis and Biotechnological Production

Cited by 42 publications

References 115 publications

Neutrophils Facing Biofilms: The Battle of the Barriers

Neutrophils Facing Biofilms: The Battle of the Barriers

Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis

The Use of Edible Films Based on Sodium Alginate in Meat Product Packaging: An Eco-Friendly Alternative to Conventional Plastic Materials

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