Alginate Biosynthesis: A Model System for Gene Regulation and Function in Pseudomonas

Deretić, Vojo; Gill, John F.; Chakrabarty, Ananda M.

doi:10.1038/nbt0587-469

Cited by 67 publications

(45 citation statements)

References 49 publications

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“…Deletion of 86 bp (pCOP7B) or 124 bp (pCOP8B) from the 5' end reduced the level of copper resistance, but resistance was elevated when thes'e derivatives were cloned with the ORFs oriented behind the lac promoter (pCOP7A and pCOP8A). This (6,10) were not observed in this region. Deletions from the 5' end into ORFs A, B, C, and D generally inactivated resistance to copper, regardless of orientation to the lac promoter.…”

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confidence: 55%

Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringae pv. tomato

1988

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The nucleotide sequence of a 4.5-kilobase copper resistance determinant from Pseudomonas syringae pv. tomato revealed four open reading frames (ORFs) in the same orientation. Deletion and site-specific mutational analyses indicated that the first two ORFs were essential for copper resistance; the last two ORFs were required for full resistance, but low-level resistance could be conferred in their absence. Five highly conserved, direct 24-base repeats were found near the beginning of the second ORF, and a similar, but less conserved, repeated region was found in the middle of the first ORF.

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confidence: 55%

Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringae pv. tomato

1988

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“…Our next objective will be to analyze transcription initiations in these cases to determine whether this conserved region is involved in promoting transcription, FIG. 10. Hybridization of KpnI-digested p1414, pDE1470, pDE1554, PPG1, PPDE Gl, and PPDE G2 DNA with 32P-labeled 646-bp EcoRI-EcoRV fragment from p1414 (A) and 32P-labeled 750-bp XhoI-HindIII fragment from pDE-Km (B).…”

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confidence: 99%

Evolutionary differences in chromosomal locations of four early genes of the tryptophan pathway in fluorescent pseudomonads: DNA sequences and characterization of Pseudomonas putida trpE and trpGDC

1990

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Pseudomonas putida possesses seven structural genes for enzymes of the tryptophan pathway. All but one, trpG, which encodes the small (beta) subunit of anthranilate synthase, have been mapped on the circular chromosome. This report describes the cloning and sequencing of P. putida trpE, trpG, trpD, and trpC. In P. putida and Pseudomonas aeruginosa, DNA sequence analysis as well as growth and enzyme assays of insertionally inactivated strains indicated that trpG is the first gene in a three-gene operon that also contains trpD and trpC. In P. putida, trpE is 2.2 kilobases upstream from the trpGDC cluster, whereas in P. aeruginosa, they are separated by at least 25 kilobases (T. Shinomiya, S. Shiga, and M. Kageyama, Mol. Gen. Genet., 189:382-389, 1983). The DNA sequence in P. putida shows an open reading frame on the opposite strand between trpE and trpGDC; this putative gene was not characterized. Evidence is also presented for sequence similarities in the 5' untranslated regions of trpE and trpGDC in both pseudomonads; the function of these regions is unknown, but it is possible that they play some role in regulation of these genes, since all the genes respond to repression by tryptophan. The sequences of the anthranilate synthase genes in the fluorescent pseudomonads resemble those of p-aminobenzoate synthase genes of the enteric bacteria more closely than the anthranilate synthase genes of those organisms; however, no requirement for p-aminobenzoate was found in the Pseudomonas mutants created in this study.

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“…A consensus promoter sequence has not been identified in Pseudomonas sp. (Deretic et al, 1987). Although no obvious promoter sequences (i.e.…”

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Cloning, sequence analysis and expression in Escherichia coli of a gene encoding an alginate lyase from Pseudomonas sp. OS-ALG-9

Maki

Mori²,

Fujiyama³

et al. 1993

Journal of General Microbiology

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Alginate Biosynthesis: A Model System for Gene Regulation and Function in Pseudomonas

Cited by 67 publications

References 49 publications

Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringae pv. tomato

Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringae pv. tomato

Evolutionary differences in chromosomal locations of four early genes of the tryptophan pathway in fluorescent pseudomonads: DNA sequences and characterization of Pseudomonas putida trpE and trpGDC

Cloning, sequence analysis and expression in Escherichia coli of a gene encoding an alginate lyase from Pseudomonas sp. OS-ALG-9

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