Alginate and chitosan particles as drug delivery system for cell therapy

Ciofani, Gianni; Raffa, Vittoria; Menciassi, Arianna; Dario, P.

doi:10.1007/s10544-007-9118-7

Cited by 48 publications

(14 citation statements)

References 23 publications

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“…Over the past few decades, controlled drug delivery technology has made substantial progress and became a hot topic in modern medication (Lesinski et al 2005;Ciofani et al 2007;Vallet-Regi et al 2007;Ciofani et al 2008). Up to now, according to the physicochemical properties of the drug encapsulated and the requirement of the clinic, various drug delivery platforms, including polymeric and inorganic matrix, have been designed and developed.…”

Section: Introductionmentioning

confidence: 99%

Effect of size on the cellular endocytosis and controlled release of mesoporous silica nanoparticles for intracellular delivery

Gan

Dai

Yuan

et al. 2011

Biomed Microdevices

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Due to the unique physicochemical properties and membrane-permeable capacity, mesoporous silica nanoparticles (MSNs) are considered as an ideal carrier for intracellular delivery. Herein, we endeavored to address the size effect of MSNs on the cellular uptake, endosomal escape and controlled release, the key steps for the intracellular delivery. The well-ordered MSNs in the range from 55-nm to 440-nm with similar pore texture were prepared by modified base-catalyzed sol-gel method. With MC3T3-E1 model cell line, the in vitro results indicated that after 12 h cultivation, MSNs within 55 ~ 440 nm could all be internalized into the cells, and further escaped out of the endosomal compartment. The efficiency of the cellular uptake and endosomal escape strongly depended on the particle size, with the best efficiencies from 100-nm MSNs. Furthermore, the MTT results indicated that these MSNs materials were all biocompatible. The controlled release experiments with hydrophobic dexamethasone and hydrophilic vitamin C as models showed that for these small-molecular drugs, the loading amount all mainly determined by the surface area of the MSNs, and the subsequent release of the drug dramatically decreased with the increasing of the particle size. By contrast, the release rate of vitamin C was much quicker than that of the dexamethasone. These findings presented here could provide new means to tailor the size of MSNs and thus to guide the design of MSNs-based intracellular delivery system. Due to the good cell biocompatibility, high cellular uptake and endosomal escape, we conjectured that the 100-nm MSNs are more favorable for the intracellular delivery of drugs in live cells.

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Section: Introductionmentioning

confidence: 99%

Effect of size on the cellular endocytosis and controlled release of mesoporous silica nanoparticles for intracellular delivery

Gan

Dai

Yuan

et al. 2011

Biomed Microdevices

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“…This gradual release profile of the entrapped drug over a prolonged period might be due to the special environment of the tumor tissue, especially the low pH and the lysosomal degradation of the chitosan NPs. Also, in the immortalized mouse hippocampal cell line HN9.10e an increased uptake of chitosan NPs could be observed and the internalization process showed first order kinetics [177].…”

Section: Utilization Of Carbohydrates For Nanoparticulate Systemsmentioning

confidence: 88%

Exploiting the Properties of Biomolecules for Brain Targeting of Nanoparticulate Systems

Cramer

Rempe²,

Galla³

2012

CMC

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The main obstacle in the treatment of central nervous system diseases is represented by a limited passage of diagnostic and therapeutic agents across the blood-brain barrier, which separates the blood stream from the cerebral parenchyma and maintains the homeostasis of the brain. The growing knowledge about the brain capillary endothelium and the discovery of specific mechanisms for the uptake of substances enables the development of various strategies to enhance the drug delivery rate into the brain. Among the different strategies, nanoparticles are promising candidates for drug delivery to the brain due to their potential in encapsulating drugs and thereby disguising their permeation limiting characteristics. Furthermore a surface functionalization of many nanoparticles can easily be achieved allowing the active targeting of nanoparticles to the brain. For this non-invasive approach, the surface functionalization of nanoparticles with biomolecules has shown promising potential for effective drug delivery to the brain. This review indexes the main classes of biomolecules used for the surface functionalization of nanoparticles and discusses their potential as drug delivery systems for an enhanced passage of diagnostic and therapeutic agents into the brain parenchyma.

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“…Alginate magnetic microspheres were developed by using a water/oil dispersion process and reticulation with calcium ions (Ciofani et al 2008a). Briefly, 75 μl of the alginate-magnetite solution was added in drops to 350 μl of an oil phase (iso-octane and Tween 85, a surfactant) and mixed with a ZX3 vortex (Velp scientific) at 2,400 rpm for 2 min.…”

Section: Microsphere Design and Characterizationmentioning

confidence: 99%

“…Size and size distribution analysis of particles were carried out by specific imaging and CAD software (Adobe PhotoShop, AutoCAD; Ciofani et al 2008a;Hanley et al 2007); five FIB images of three different batches of microspheres were analysed and the size distribution was obtained with about 1,500 microspheres.…”

Section: Microsphere Design and Characterizationmentioning

confidence: 99%

Magnetic alginate microspheres: system for the position controlled delivery of nerve growth factor

Ciofani

Raffa

Menciassi

et al. 2008

Biomed Microdevices

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The use of polymeric carriers containing dispersed magnetic nanocrystalline particles for targeted delivery of drugs in clinical practice has attracted the interest of the scientific community. In this paper a system comprised of alginate microparticles with a core of magnetite and carrying nerve growth factor (NGF) is described. The magnetic properties of these microspheres, typical of superparamagnetic materials, allow precise and controlled delivery to the intended tissue environment. Experiments carried out on PC12 cells with magnetic alginate microspheres loaded with NGF have confirmed the induction of cell differentiation which is strongly dependent on the distance from the microsphere cluster. In addition, finite element modelling (FEM) of the release profile from the microspheres in culture, indicated the possibility of creating defined and predictable NGF gradients from the loaded microspheres. These observations on the carriage and release of growth factors by the proposed microparticles open new therapeutic options for both neuronal regeneration and of the development of effective neuronal interfaces.

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Alginate and chitosan particles as drug delivery system for cell therapy

Cited by 48 publications

References 23 publications

Effect of size on the cellular endocytosis and controlled release of mesoporous silica nanoparticles for intracellular delivery

Effect of size on the cellular endocytosis and controlled release of mesoporous silica nanoparticles for intracellular delivery

Exploiting the Properties of Biomolecules for Brain Targeting of Nanoparticulate Systems

Magnetic alginate microspheres: system for the position controlled delivery of nerve growth factor

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