Algerian watermelon mosaic virus (AWMV): a new potyvirus species in the PRSV cluster

Abstract

A potyvirus was isolated from a naturally infected squash plant in Algeria in 1986. Biological and serological data have revealed that the virus, initially described as H4, is related to other cucurbit-infecting potyviruses, particularly Moroccan watermelon mosaic virus (MWMV) and Papaya ringspot virus (PRSV). To establish unequivocally the taxonomic status of H4, its full-length genome sequence was established. H4 shared identities of 70% and 65% at the amino acid level with MWMV and PRSV, respectively, indicating that H4 is a distinct species of the PRSV cluster. The name Algerian watermelon mosaic virus (AWMV) is proposed for this new potyvirus species.

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

“…Using biological and serological data on 20 PRSV-related isolates, Quiot-Douine et al defined at least seven related isolate groups (QuiotDouine et al, 1990). Subsequent molecular analysis indicated that five of these seven groups correspond to acknowledged species: PRSV, Moroccan watermelon mosaic virus (MWMV) (Lecoq et al, 2001), Algerian watermelon mosaic virus (AWMV) (Yakoubi et al, 2008b), Zucchini yellow fleck virus (ZYFV) (Desbiez et al, 2007) and Zucchini tigre mosaic virus (ZTMV) (Romay et al, 2014). Other species, different from the seven previously studied groups, have subsequently been characterized in the PRSV cluster.…”

“…Reverse transcription, PCR amplification and sequencing of Su03-07 genome were performed as previously described for members of the PRSV clusters, using 3 pairs of "potyvirus" primers (Colinet et al, 1998;Gibbs and Mackenzie, 1997;Ha et al, 2008), one primer PRSV-5NC-5' designed at the 5' extremity of several members of the PRSV cluster (Romay et al, 2014;Yakoubi et al, 2008a;Yakoubi et al, 2008b), and specific internal primers as needed, designed from the partial sequences obtained with the generic primers. Sequence assembly was performed with CAP3 (Huang and Madan, 1999) with manual corrections when needed.…”

“…MWMV is present in Africa (Lecoq and Desbiez, 2012;Yakoubi et al, 2008a) and has been recently introduced in southern Europe (Lecoq and Desbiez, 2012 ;Malandraki et al, 2015), but it has not been found on other continents (Lecoq and Desbiez, 2012). AWMV was detected once in Algeria in 1986 and once in Nigeria in 2005(Yakoubi et al, 2008b, Owolabi et al, 2011. ZYFV was observed occasionally in Crete, Israel and Italy in melon crops (Antignus et al, 1995;Avgelis, 1985;Tomassoli et al, 2010) and in France, where it is mostly confined to the wild cucurbits Ecballium elaterium and Bryonia dioica (Lecoq and Desbiez, 2012).…”

High-throughput sequencing of virus-infected Cucurbita pepo samples revealed the presence of Zucchini shoestring virus in Zimbabwe

“…Moreover, MWMV, AWMV, ZSSV, SuWMV and WMVBV were identified in Africa, suggesting that the PRSV cluster underwent an important diversification in Africa [25]. Out of these viruses present in Africa, MWMV is the widespread one having been reported in all African regions [3,[26][27][28][29][30][31][32]. The HTS in this study made the detection of ZSSV on infected leaf sample possible.…”

Susceptibility assessment of watermelon and pumpkin cultivars to potyvirus in Tocantins

“…According to Yakoubi et al (2008), the predominance of PRSV-W was probably due to its virulence and plant x vector interaction. Several studies have reported differences in the interactions between viruses in laboratory and field conditions associated with the host plant and the interaction 1541 Susceptibility assessment… OOTANI, M. A. et al between the insect and vector.…”