Algal growth and utilization of phosphorus studied by combined mono-culture and co-culture experiments

Flow Regime and Nutrient-Loading Trends from the Largest South European Watersheds: Implications for the Productivity of Mediterranean and Black Sea’s Coastal Areas

“…Despite it does not include other processes that supply new nutrients in the coastal zones (e.g., upwelling of deeper waters, wastewater loads, atmospheric deposition), as well as the regenerated production, this calculation highlights the role of the rivers to induce nitrogen or phosphorus limitations of the new production in the coastal zones and to favor (depress) the growth of non-siliceous algal species delivering large (scarce) quantities of both these nutrients compared to silicon. Moreover, considering the ability of several autotrophic plankton species to use organic phosphorus for their growth after enzymatic hydrolysis [57,58], the eutrophication potential of DIN was compared to both those of PO 4 3− and TP.…”

“…For all the rivers, EP based on the river loads of DIN was higher than that of PO 4 3− , suggesting that the supply of land-borne inorganic phosphorus was fundamentally the factor limiting the new production in the coastal marine environment ( Figure 5). However, the scarcity of phosphorus could be mitigated by the fraction of the organic phosphorus pool potentially bioavailable for algal growth [57,58]. During the last two decades, this compensation was potentially greater for the Rhône and Po (TP-based production respectively equal to 61% and 79% of DIN-based production) compared to the Ebro and Danube (TP-based production equal to 45% and 54% of DIN-based production).…”

“…Nevertheless, a high concentration of bioavailable particulate phosphorus was found in the Danube Delta [78]. Moreover, many freshwater and marine algae species are able to synthesize alkaline phosphatase to obtain PO 4 3− by the enzymatic hydrolysis of phosphormonoesters, which are an important component of the natural pool of dissolved organic phosphorus [57]. This enzymatic catalysis can significantly sustain phytoplankton productivity in the marine environments characterized by a persistent deprivation of PO 4 3− , like the Northern Adriatic [58].…”

Ecotoxicological effects of enrofloxacin and its removal by monoculture of microalgal species and their consortium

“…The remediation of EOCs by microbial consortia can be very advantageous because synergistic relationships between the co-cultivated microorganisms can be constructed, enhancing the overall uptake efficiency. The microbial consortia systems are more resistant to changes in environmental conditions, since the multi-microorganism species with distinct metabolic activities that are adapted to diverse environmental variations was found to allow the development of a robust biological system that can operate under different environmental stresses (B acsi et al, 2016;Ren et al, 2017).…”

An Experimental Insight into Extracellular Phosphatases – Differential Induction of Cell-Specific Activity in Green Algae Cultured under Various Phosphorus Conditions

“…An early application of the ELF ® 97 Endogenous Phosphatase Detection Kit successfully visualized phosphatase-positive cells in both algal cultures and natural phytoplankton, i.e., directly tagged the P-limited algal species ( González-Gil et al, 1998 ; Dyhrman and Palenik, 1999 ; Rengefors et al, 2001 ), although further studies revealed some uncertainties and/or potential misinterpretations (e.g., Rengefors et al, 2003 ; Dignum et al, 2004 ; Ou et al, 2010 ). The principle shortcoming of the ELF ® 97 method as it is applied in recent studies, including the original paper by González-Gil et al (1998) , is that only the occurrence of ELFA-labeling, i.e., merely qualitative estimates of phosphatase-positive algal cells and/or species, presence/absence of tagged phytoplankton, etc., could be reported ( Štrojsová et al, 2003 ; Cao et al, 2005 ; Rychtecký et al, 2015 ; Ren et al, 2017 ). The only quantification of phosphatase activity accessible within the limits of this original method is to score a percentage of ELFA-labeled cells (e.g., Rengefors et al, 2003 ; Dyhrman and Ruttenberg, 2006 ; Litchman and Nguyen, 2008 ; Young et al, 2010 ).…”