Algal extracellular organic matter pre-treatment enhances microalgal biofilm adhesion onto microporous substrate

Tong, C. Y.; Lew, J.K.; Chan, Derek Juinn Chieh

doi:10.1016/j.chemosphere.2022.135740

Cited by 16 publications

(8 citation statements)

References 47 publications

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“…This process ultimately promotes irreversible cell adhesion onto the membranes [ 17 , 35 ]. Apart from this, our previous findings have shown that bounded polysaccharides were mainly responsible for cell adhesion in N. incerta too [ 11 ].…”

Section: Resultsmentioning

confidence: 83%

“…From the observed cell adhesion trend and biofilm morphological characteristics difference above, presence of AOM bio-coating has successfully demonstrated the enhancement of cell deposition and growth profile variation which was most likely affected by a wide range of environmental conditions or perhaps, due to the cell adaptation to different types of conditioning layer [ 11 ]. In this context, it is important to further probe into their respective AOM production rates to examine the cell response or mechanisms toward the bio-coating.…”

Section: Resultsmentioning

confidence: 99%

“…culture’s EPS was shown to be the best substratum to support the highest adhesion capability of around 94.60% [ 10 ]. Investigations from our former study demonstrated that cell binding affinity of three marine microalgae, namely Amphora coffeaeformis , Cylindrotheca fusiformis , and Navicula incerta, was 10 times greater on EPS-coated membranes with specific cell adaptation period toward the bio-coatings derived from different microalgae species [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Uncovering the role of algal organic matter biocoating on Navicula incerta cell deposition and biofilm formation

Tong,

Lim,

Chua

et al. 2023

Bioengineered

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Spontaneous natural biofilm concentrates microalgal biomass on solid supports. However, the biofilm is frequently susceptible to exfoliation upon nutrient deficiency, particularly found in aged biofilm. Therefore, this study highlights a novel biofilm cultivation technique by pre-depositing the algal organic matters from marine diatom, Navicula incerta onto microporous polyvinylidene fluoride membrane to further strengthen the biofilm developed. Due to the improvement in membrane surface roughness and hydrophobicity, cells adhered most abundantly to soluble extrapolymeric substances-coated (sEPS) (76 10 6 16 10 6 cells m −2 ), followed by bounded EPS-coated (57.67 10 6 0.33 10 6 cells m −2 ), internally organic matter (IOM)-coated (39.00 10 6 5.19 10 6 cells m −2 ), and pristine control the least (6.22 10 6 0.77 10 6 cells m −2 ) at 24 th h. Surprisingly, only bEPS-coated membrane demonstrated an increase in cell adhesion toward the end of the experiment at 72 h. The application of the bio-coating has successfully increased the rate of cell attachment by at least 45.3% upon inoculation and achieved as high as 89.9% faster attachment at 72 hours compared to the pristine control group. Soluble polysaccharides and proteins might be carried along by the cells adhering onto membranes hence resulting in a built up of EPS hydrophobicity (>70% in average on bio-coated membranes) over time as compared with pristine (control) that only recorded an average of approximately 50% hydrophobicity. Interestingly, cells grown on bio-coated membranes accumulated more internally bounded polysaccharides, though bio-coating had no discernible impact on the production of both externally and internally bounded protein. The collective findings of this study reveal the physiological alterations of microalgal biofilms cultured on bio-coated membranes.

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Section: Resultsmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Uncovering the role of algal organic matter biocoating on Navicula incerta cell deposition and biofilm formation

Tong,

Lim,

Chua

et al. 2023

Bioengineered

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“…Besides, EPS are the components of biofilms, which can control adhesion and protect bacteria from harsh environments (Morales-Garcia, Bailey, Jana, & Burgess, 2019). EPS accumulating on cell surfaces strengthened biofilm adhesion by forming a regulatory membrane (Cheah & Chan, 2021;Tong, Lew, & Derek, 2022;Mayton, Marcus, & Walker, 2019). For example, the accumulation of EPS secreted by E. coli O157:H7 on glass surfaces strengthened the growth and development of biofilm (Oh, Jo, Yang, & Park, 2007).…”

Section: Discussionmentioning

confidence: 99%

The effects of freezing-induced sublethally injury and recovery on adhesion of Escherichia coli O157:H7

Zhai,

Zhang,

Chen

et al. 2023

Preprint

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Freezing as an effective approach for food storage is commonly used in food industry. Notably, Escherichia coli O157:H7 can survive in a sublethally injured state after freezing and recover under suitable conditions, which is a great threat. This research was to investigate changes and mechanism in adhesion of sublethally injured E. coli O157:H7 during freezing and recovery. Adhesion on stainless steel or lettuce surface showed an increase after 16 h-freezing with sublethal ratio of more than 99%. Adhesion recovered to untreated level in recovery on lettuce while didn’t recover on stainless steel. Motility decreased as freezing time increasing, reached minimum after 12 h-freezing and didn’t recover to untreated level under recovery conditions, which was determined by diameter of motility halo. Extracellular polymeric substances production and carbohydrate to protein ratio both reached minimum after 4 h-freezing and then increased. No significant change of LPS structural feature or gene expression was observed during injury and recovery. Besides, the expressions of flagella, pili and EPS related genes were all down-regulated during freezing while were up-regulated after recovery. The results revealed adhesion behavior of freezing-induced sublethally injured E. coli O157:H7, which provides theoretical basis to bacterial prevention.

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“…It was discovered that the physiological behavior of microalgae was the most dynamic within the first six hours right after cell inoculation and liquid channeling which has been established in our previous studies. 13,14 It was dubbed as the "golden period" for identification of architectural and physiological transitions to examine the cell persistence on solid substrates. However, knowledge about the possibility of cell detachment during that particular in-contact period has not been reported anywhere, and the current understanding of cell detachment is largely derived from macroscopic field observations using batch and chemostat systems, which are too generalized to be used and are not specifically created to effectively optimize operating parameters.…”

Section: Introductionmentioning

confidence: 99%

A microscale system for in situ investigation of immobilized microalgal cell resistance against liquid flow in the early inoculation stage

Cheah¹,

Li²,

Chan³

2023

Lab Chip

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Algal extracellular organic matter pre-treatment enhances microalgal biofilm adhesion onto microporous substrate

Cited by 16 publications

References 47 publications

Uncovering the role of algal organic matter biocoating on Navicula incerta cell deposition and biofilm formation

Uncovering the role of algal organic matter biocoating on Navicula incerta cell deposition and biofilm formation

The effects of freezing-induced sublethally injury and recovery on adhesion of Escherichia coli O157:H7

A microscale system for in situ investigation of immobilized microalgal cell resistance against liquid flow in the early inoculation stage

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