Algal Biotechnology: A Sustainable Route for Omega-3 Fatty Acid Production

Dhanya, B.; Sowmiya, Gandhi; Jeslin, J.; Chamundeeswari, M.; Verma, Madan L.

doi:10.1007/978-981-15-0169-2_4

Cited by 6 publications

(4 citation statements)

References 60 publications

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“…maximum accumulation of linolenic acid (50%) and linoleic acid (37%) was observed in F/2 media, followed by MKM and MASM media. The differences in types of fatty acid accumulation in different media support earlier findings in which nutrients were found to regulate FA biosynthesis (Ren et al, 2010;Dhanya et al, 2020). Thus, selection of optimum media should be carried out before attenuation of specific stress factors for enhancing targeted fatty acids.…”

Section: Effect Of Different Media On Growth and Fatty Acid Profilingsupporting

confidence: 81%

“…In order to combat diverse environments, specifically higher salinity conditions and low nitrogen availability, microalgae alters or enhances the PUFA production (Dhanya et al, 2020). Enhancement in the TAG content (Kan et al, 2012) and intracellular lipids in microalgae (Zhila et al, 2011) are also reported for salinity stress.…”

Section: Effect Of Different Media On Growth and Fatty Acid Profilingmentioning

confidence: 99%

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Bioprospecting Indigenous Marine Microalgae for Polyunsaturated Fatty Acids Under Different Media Conditions

Jain

Minhas

Shukla

et al. 2022

Front. Bioeng. Biotechnol.

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Marine microalgae produce a number of valuable compounds that have significant roles in the pharmaceutical, biomedical, nutraceutical, and food industries. Although there are numerous microalgal germplasms available in the marine ecosystem, only a small number of strains have been recognized for their commercial potential. In this study, several indigenous microalgal strains were isolated from the coast of the Arabian Sea for exploring the presence and production of high-value compounds such as polyunsaturated fatty acids (PUFAs). PUFAs are essential fatty acids with multiple health benefits. Based on their high PUFA content, two isolated strains were identified by ITS sequencing and selected for further studies to enhance PUFAs. From molecular analysis, it was found both the strains were green microalgae: one of them was a Chlorella sp., while the other was a Planophila sp. The two isolated strains, together with a control strain known for yielding high levels of PUFAs, Nannochloropsis oculata, were grown in three different nutrient media for PUFA augmentation. The relative content of α-linolenic acid (ALA) as a percentage of total fatty acids reached a maximum of 50, 36, and 50%, respectively, in Chlorella sp., Planophila sp., and N. oculata. To the best of our knowledge, this is the first study in exploring fatty acids in Planophila sp. The obtained results showed a higher PUFA content, particularly α-linolenic acid at low nutrients in media.

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Section: Effect Of Different Media On Growth and Fatty Acid Profilingsupporting

confidence: 81%

Section: Effect Of Different Media On Growth and Fatty Acid Profilingmentioning

confidence: 99%

Bioprospecting Indigenous Marine Microalgae for Polyunsaturated Fatty Acids Under Different Media Conditions

Jain

Minhas

Shukla

et al. 2022

Front. Bioeng. Biotechnol.

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“…With people's concerns about the sustainability of fish, algae, and microalgae with single cells as new sources for producing ω−3, PUFAs have become research hotspots in recent years. Relevant reviews have already been discussed about the production method of long‐chain PUFAs (docosahexaenoic acid/eicosapentaenoic acid [DHA/EPA]) derived from algae and microalgae with single cells (Dhanya et al., 2020; Diao et al., 2020; Russo et al., 2021). In addition, plant‐derived PUFAs (α‐linolenic acid/stearic acid [ALA/SDA]) have also become more and more popular.…”

Section: Introductionmentioning

confidence: 99%

Omega‐3 polyunsaturated fatty acid encapsulation system: Physical and oxidative stability, and medical applications

Zhou

et al. 2022

Food Frontiers

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The benefits of omega-3 (ω-3) polyunsaturated fatty acids (PUFAs) to human health and the therapeutic properties of diseases have been widely identified. However, due to abundant double bonds, they are sensitive to environmental conditions which cause oxidative deterioration. This is one of the main factors limiting their application. The encapsulation of ω-3 PUFAs is an effective way to improve their oxidation stability. This review summarizes the recent advances in different forms of ω-3 encapsulation systems, such as emulsions, liposomes, microcapsules, and liquid crystalline. Particular emphasis is given to the effects of food-derived biopolymers wall materials (proteins, polysaccharides, and esters) on encapsulation efficiency and oxidation stability of ω-3 PUFAs encapsulation systems. In addition, the effects of natural antioxidants (polyphenols, carotenoids, flavonoids, plant essential oils, etc.) on the antioxidant enhancement of ω-3 PUFAs encapsulation system based on food-derived biopolymers are discussed.Different forms of ω-3 PUFAs encapsulation systems have been proven to be used as supplements in the medical field. The regulation and improvement of the ω-3 PUFAs encapsulation system on the diseases of animal and human, which are related to central nervous system, retina, lung, kidney and tissue cell, are systematically discussed.

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“…DHA is essential for growth and functional development of the brain in infants and the maintenance of normal brain function in adults (Horrocks and Yeo 1999). Although fish oils represent the main dietary source of EPA and DHA, alternative sources of these omega-3 fatty acids are required owing to increasing marine pollution and depleting wild fish stocks (Doughman et al 2007, Martins et al 2013, Dhanya et al 2020. Some marine protists are known to have high EPA and DHA contents; for example, the eustimatophyte Nannochloropsis spp.…”

Section: Introductionmentioning

confidence: 99%

Semi-continuous cultivation of the mixotrophic dinoflagellate Gymnodinium smaydae, a new promising microalga for omega-3 production

Lim¹,

Jeong²,

You³

et al. 2020

ALGAE

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Omega-3 fatty acids, including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are polyunsaturated fatty acids beneficial to human health. A limited number of microalgae have been used for commercial omega-3 production, which necessitates the identification of new microalgae with high omega-3 contents. We explored the fatty acid composition and EPA and DHA contents of the mixotrophic dinoflagellate Gymnodinium smaydae fed with the optimal algal prey species Heterocapsa rotundata. Cells of G. smaydae were found to be rich in omega-3 fatty acids. In particular, the DHA content of G. smaydae was 21 mg g-1 dry weight, accounting for 43% of the total fatty acid content. The percentage of DHA in the total fatty acid content of G. smaydae was the highest among the reported microalgae except for Crypthecodinium cohnii. Moreover, to determine if the prey supply interval affected the growth rate of G. smaydae and its fatty acid content, three different prey supply intervals (daily, once every 2 d, and once for 4 d) were tested. Daily prey supply yielded the highest total fatty acid and DHA contents in G. smaydae. Furthermore, we successfully produced high-density G. smaydae cultures semi-continuously for 43 d with daily prey supply. During the semi-continuous cultivation period, the highest density of G. smaydae was 57,000 cells mL-1 , with an average growth rate of 0.7 d-1. Taken together, the percentage of EPA and DHA in the total fatty acid content was maintained in the range of 54.2-56.9%. The results of this study support G. smaydae as a promising microalgal candidate for commercial DHA production and demonstrate that daily supply of prey can efficiently produce high-density G. smaydae cultures for more than a month.

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Algal Biotechnology: A Sustainable Route for Omega-3 Fatty Acid Production

Cited by 6 publications

References 60 publications

Bioprospecting Indigenous Marine Microalgae for Polyunsaturated Fatty Acids Under Different Media Conditions

Bioprospecting Indigenous Marine Microalgae for Polyunsaturated Fatty Acids Under Different Media Conditions

Omega‐3 polyunsaturated fatty acid encapsulation system: Physical and oxidative stability, and medical applications

Semi-continuous cultivation of the mixotrophic dinoflagellate Gymnodinium smaydae, a new promising microalga for omega-3 production

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