Algae-based oral recombinant vaccines

Specht, Elizabeth A.; Mayfield, Stephen P.

doi:10.3389/fmicb.2014.00060

Cited by 111 publications

(73 citation statements)

References 44 publications

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“…The resulting purified protein from the A/PR/8/34 (H1N1) strain was found in mice to be immunogenic and protective against a lethal challenge with homologous virus. 116 Subunit recombinant vaccines expressed in microalgae are candidates of choice for oral administration, 117 the system allowing for production of complex antigens that elicit immunogenic responses, while numerous reports have identified algae-derived compounds as immunomodulatory molecules. 107 For example, the D2 fibronectin-binding domain of Staphylococcus, fused to the cholera toxin B and expressed from the chloroplast of C. reinhardtii, was shown to be immunogenic in mice after oral administration of lyophilized alga powder.…”

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confidence: 99%

Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules

Legastelois

Buffin

Peubez

et al. 2016

Human Vaccines & Immunotherapeutics

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The increasing demand for recombinant vaccine antigens or immunotherapeutic molecules calls into question the universality of current protein expression systems. Vaccine production can require relatively low amounts of expressed materials, but represents an extremely diverse category consisting of different target antigens with marked structural differences. In contrast, monoclonal antibodies, by definition share key molecular characteristics and require a production system capable of very large outputs, which drives the quest for highly efficient and cost-effective systems. In discussing expression systems, the primary assumption is that a universal production platform for vaccines and immunotherapeutics will unlikely exist. This review provides an overview of the evolution of traditional expression systems, including mammalian cells, yeast and E.coli, but also alternative systems such as other bacteria than E. coli, transgenic animals, insect cells, plants and microalgae, Tetrahymena thermophila, Leishmania tarentolae, filamentous fungi, cell free systems, and the incorporation of non-natural amino acids.

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mentioning

confidence: 99%

Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules

Legastelois

Buffin

Peubez

et al. 2016

Human Vaccines & Immunotherapeutics

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“…For each P450, its scaffold number, DNA strand and number of introns lutionary history of earth and became major players in global energy / biomass production and biogeochemical recycling (Grossman 2005, Blanc et al 2010. Among algae, research on microalgae has been intensified because of their enormous biotechnological potential, including generation of biofuels (Behera et al 2015), phytochemicals (De Jesus Raposo and De Morais 2015), cosmetics (Wang et al 2015) and vaccines (Specht and Mayfield 2014) and their use in wastewater treatment (Tiron et al 2015). Microalgae are also used as biomarkers for organic and inorganic pollution (Torres et al 2008).…”

Section: Methodsmentioning

confidence: 99%

Cytochrome P450 monooxygenase analysis in free-living and symbiotic microalgae Coccomyxa sp. C-169 and Chlorella sp. NC64A

Mthakathi¹,

Kgosiemang²,

Chen³

et al. 2015

ALGAE

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Microalgae research is gaining momentum because of their potential biotechnological applications, including the generation of biofuels. Genome sequencing analysis of two model microalgal species, polar free-living Coccomyxa sp. C-169 and symbiotic Chlorella sp. NC64A, revealed insights into the factors responsible for their lifestyle and unravelled biotechnologically valuable proteins. However, genome sequence analysis under-explored cytochrome P450 monooxygenases (P450s), heme-thiolate proteins ubiquitously present in species belonging to different biological kingdoms. In this study we performed genome data-mining, annotation and comparative analysis of P450s in these two model algal species. Sixty-nine P450s were found in two algal species. Coccomyxa sp. showed 40 P450s and Chlorella sp. showed 29 P450s in their genome. Sixty-eight P450s (>100 amino acid in length) were grouped into 32 P450 families and 46 P450 subfamilies. Among the P450 families, 27 P450 families were novel and not found in other biological kingdoms. The new P450 families are CYP745-CYP747, CYP845-CYP863, and CYP904-CYP908. Five P450 families, CYP51, CYP97, CYP710, CYP745, and CYP746, were commonly found between two algal species and 16 and 11 P450 families were unique to Coccomyxa sp. and Chlorella sp. Synteny analysis and gene-structure analysis revealed P450 duplications in both species. Functional analysis based on homolog P450s suggested that CYP51 and CYP710 family members are involved in membrane ergosterol biosynthesis. CYP55 and CYP97 family members are involved in nitric oxide reduction and biosynthesis of carotenoids. This is the first report on comparative analysis of P450s in the microalgal species Coccomyxa sp. C-169 and Chlorella sp. NC64A.Key Words: carotenoid biosynthesis; Chlorella sp. NC64A; Coccomyxa sp. C-169; cytochrome P450 monooxygenases; nitric oxide reductase; P450nor; symbiosis INTRODUCTIONGreen algae belonging to the phylum Chlorophyta consist of microorganisms adapted to diverse ecological niches. Green algae are photosynthetic in nature and it is estimated that more than one billion years ago terrestrial plants emerged from these organisms (Heckman et al. 2001). These organisms played a key role during the evoReceived April 3, 2015, Accepted July 6, 2015 *Corresponding Author E-mail: khajamohiddinsyed@gmail.com Tel: This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 234 MATERIALS AND METHODSThe genomes of Coccomyxa sp. C-169 and Chlorella sp. NC64A have been sequenced and their genome sequence data are available for public use (Blanc et al. 2010(Blanc et al. , 2012. Genome data mining of P450s in the two species was carried out as described elsewhere (Kgosiemang et al. 2014. Briefly, the proteomes of these species were downloaded from the respective databases...

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“…Some of the biotech plants, including microalgae, used for the production platforms of a number of biopharmaceuticals are listed in Table 6.2. For more detailed listings of plant-based biopharmaceuticals see reviews by Daniell et al (2009), Walsh (2010, Obeme et al (2011), Da Cunha et al (2014, and Specht and Mayfield (2014). …”

Section: Molecular Farmingmentioning

confidence: 99%

Next Generation Plant Biotechnology

Ahuja¹

2014

Sustainable Development and Biodiversity

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Modern plant biotechnology began with the transfer of foreign chimeric genes into plants. Initially recombinant genes were derived from bacteria, animals and plants for gene transfer. Gene transfer was accomplished by Agrobacteriummediated or biolistic methods into the plant genome. The first wave of transgenic plants that were monitored for transgene integration and expression, the second wave transgenic plants carried economically important genes for herbicide tolerance, pest resistance, drought and salt tolerance, growth traits, and flowering control. Subsequently, a number of genetically modified crops with several useful traits have been commercialized. Although relatively stable transgene expression has been observed in a number of plant species, there were also unintended unstable events in transgenic plants. This is due to the fact that transgene integration achieved by the two traditional methods ( Agrobacterium or biolistic) of gene transfer in the plant genome is random, and one to several copies of the transgenes may be integrated at one or several locations in the genome. In order to overcome the problem of randomness of transgene integration, site-specific transgene integration strategies have been experimentally tested in plants, and offer prospects of stable gene integration and expression in transgenic plants. In order to broaden the scope of transgenic plants, biotechnologists started looking for other useful avenues for their utility. With finite reserves of fossil fuels and climate change, and growing demands for fuels, plastics, and pharmaceuticals, transgenic plants have been also explored as production platforms for these commodities. This paper is an overview of next generation transgenic plants that can serve as bioreactors or biofactories for the cost-effective production of biofuels, biopharmaceuticals, bioplastics, and as a resource for nutritional supplements to meet human demands in the future. New developments in nanobiotechnology offer prospects for improved production of crop plants.

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Algae-based oral recombinant vaccines

Cited by 111 publications

References 44 publications

Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules

Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules

Cytochrome P450 monooxygenase analysis in free-living and symbiotic microalgae Coccomyxa sp. C-169 and Chlorella sp. NC64A

Next Generation Plant Biotechnology

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