“…The alkaloid extract was dissolved in 2 mL of chloroform, then 25 mL of 0.02 N H 2 SO 4 were added, The resulting solution was warmed to driven off the chloroform, cooled and titrated back the excess acid with 0.02 N NaOH solution, using methyl red as an indicator. Each ml of H 2 SO 4 (0.02 N) was equivalent to 5.78 mg of alkaloid.…”