Alfred Sturtevant Walks into a <i>Bar</i>: Gene Dosage, Gene Position, and Unequal Crossing Over in <i>Drosophila</i>

Wolfner, Mariana F.; Miller, Danny E.

doi:10.1534/genetics.116.195891

Cited by 10 publications

(9 citation statements)

References 34 publications

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“…Instead, loss of one tandem repeat may be responsible for reversion. Genotypic and the associated phenotypic change patterns similar to those of PvIND and pod strings have been identified in other systems, such as the Bar gene of Drosophila (Sturtevant & Morgan, 1923;Sturtevant, 1925;Wolfner & Miller, 2016). Tandem duplication of Bar leads to dominant overexpression of the gene and a 'bar'type eye shape.…”

Section: Pvind Tandem Duplication As the Source Of Instability And Po...mentioning

confidence: 88%

Loss of pod strings in common bean is associated with gene duplication, retrotransposon insertion and overexpression of PvIND

et al. 2022

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Fruit development has been central in the evolution and domestication of flowering plants. In common bean (Phaseolus vulgaris), the principal global grain legume staple, two main production categories are distinguished by fibre deposition in pods: dry beans, with fibrous, stringy pods; and stringless snap/green beans, with reduced fibre deposition, which frequently revert to the ancestral stringy state. Here, we identify genetic and developmental patterns associated with pod fibre deposition.Transcriptional, anatomical, epigenetic and genetic regulation of pod strings were explored through RNA-seq, RT-qPCR, fluorescence microscopy, bisulfite sequencing and wholegenome sequencing.Overexpression of the INDEHISCENT ('PvIND') orthologue was observed in stringless types compared with isogenic stringy lines, associated with overspecification of weak dehiscencezone cells throughout the pod vascular sheath. No differences in DNA methylation were correlated with this phenotype. Nonstringy varieties showed a tandemly direct duplicated PvIND and a Ty1-copia retrotransposon inserted between the two repeats. These sequence features are lost during pod reversion and are predictive of pod phenotype in diverse materials, supporting their role in PvIND overexpression and reversible string phenotype.Our results give insight into reversible gain-of-function mutations and possible genetic solutions to the reversion problem, of considerable economic value for green bean production.

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Section: Pvind Tandem Duplication As the Source Of Instability And Po...mentioning

confidence: 88%

Loss of pod strings in common bean is associated with gene duplication, retrotransposon insertion and overexpression of PvIND

et al. 2022

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“…The concept of genomic environment influencing the expression of a locus is not a recent one. The famous D. melanogaster studies by Alfred Sturtevant , and by Herrmann Muller and colleagues investigating the genetic control of the fruit fly bar and white eyes phenotypes, led to the elaboration of the “position effect” (PE), a concept well described in . PE posits that some differences in phenotype can arise not from modifications of a locus's genetic information, but instead from the structural reorganization or the physical displacement of the locus into another genomic area.…”

Section: Importance Of Genomic Context On Tdna Expressionmentioning

confidence: 99%

“…A well‐known example concerns the close genomic association of some tDNAs with small nuclear (sn) or nucleolar (sno) RNAs. In Leishmania species, a U2 snRNA gene has been shown to be encoded near an alanine tDNA as well as a tRNA‐like gene, and the expression of this snRNA appears to be highly dependent on the cis regulatory elements (A and B boxes) of tDNAs . Interestingly, both the tRNA‐like and the tRNA Ala are expressed in conjunction with the U2 snRNA in these organisms.…”

Section: Importance Of Genomic Context On Tdna Expressionmentioning

confidence: 99%

The multi‐faceted regulation of nuclear tRNA gene transcription

2019

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Transfer RNAs are among the most ancient molecules of life on earth. Beyond their crucial role in protein synthesis as carriers of amino acids, they are also important players in a plethora of other biological processes. Many debates in term of biogenesis, regulation and function persist around these fascinating non‐coding RNAs. Our review focuses on the first step of their biogenesis in eukaryotes, i.e. their transcription from nuclear genes. Numerous and complementary ways have emerged during evolution to regulate transfer RNA gene transcription. Here, we will summarize the different actors implicated in this process: cis‐elements, trans‐factors, genomic contexts, epigenetic environments and finally three‐dimensional organization of nuclear genomes. © 2019 IUBMB Life, 2019 © 2019 IUBMB Life, 71(8):1099–1108, 2019

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“…Of course, tandem segmental duplications are nothing new. The original and most famous example is that of the Drosophila Bar locus (Wolfner and Miller 2016). In this classic textbook case, the Bar eye phenotype is associated with a tandem duplication encompassing seven bands in the 16A section of the Drosophila X chromosome (Bridges 1936;Sutton 1943).…”

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confidence: 99%