Alfalfa (Medicago sativa L.) MsCML46 gene encoding calmodulin-like protein confers tolerance to abiotic stress in tobacco

Du, Binghao; Chen, Naiyu; Song, Lili; Wang, Dan; Cai, Hongsheng; Lin, Yao; Li, Xiuting; Guo, Changhong

doi:10.1007/s00299-021-02757-7

Cited by 22 publications

(12 citation statements)

References 95 publications

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“…The pathway included multiple genes that respond to salt stress, such as calmodulin (CAM4), mitogenactivated protein kinase 3 (MPK3), and WRKY transcription factor 33 (WRKY33). The overexpression of MsCML46 in tobacco can lead to enhanced tolerance to multiple stresses, namely, drought, freezing, and salt stress (Du et al, 2021). In potatoes, it is confirmed that the overexpression of MPK3 can enhance osmosis and salinity tolerances by modulating the antioxidant system and proline biosynthesis (Zhu et al, 2020).…”

Section: Discussionmentioning

confidence: 90%

Comparative Transcriptome Analysis Unravels Defense Pathways of Fraxinus velutina Torr Against Salt Stress

et al. 2022

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Fraxinus velutina Torr with high salt tolerance has been widely grown in saline lands in the Yellow River Delta, China. However, the salt-tolerant mechanisms of F. velutina remain largely elusive. Here, we identified two contrasting cutting clones of F. velutina, R7 (salt-tolerant), and S4 (salt-sensitive) by measuring chlorophyll fluorescence characteristics (Fv/Fm ratio) in the excised leaves and physiological indexes in roots or leaves under salt treatment. To further explore the salt resistance mechanisms, we compared the transcriptomes of R7 and S4 from leaf and root tissues exposed to salt stress. The results showed that when the excised leaves of S4 and R7 were, respectively, exposed to 250 mM NaCl for 48 h, Fv/Fm ratio decreased significantly in S4 compared with R7, confirming that R7 is more tolerant to salt stress. Comparative transcriptome analysis showed that salt stress induced the significant upregulation of stress-responsive genes in R7, making important contributions to the high salt tolerance. Specifically, in the R7 leaves, salt stress markedly upregulated key genes involved in plant hormone signaling and mitogen-activated protein kinase signaling pathways; in the R7 roots, salt stress induced the upregulation of main genes involved in proline biosynthesis and starch and sucrose metabolism. In addition, 12 genes encoding antioxidant enzyme peroxidase were all significantly upregulated in both leaves and roots. Collectively, our findings revealed the crucial defense pathways underlying high salt tolerance of R7 through significant upregulation of some key genes involving metabolism and hub signaling pathways, thus providing novel insights into salt-tolerant F. velutina breeding.

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Section: Discussionmentioning

confidence: 90%

Comparative Transcriptome Analysis Unravels Defense Pathways of Fraxinus velutina Torr Against Salt Stress

et al. 2022

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“…Alfalfa (Medicago sativa) MsCML10 regulates cold tolerance through activating MsGSTU8 and MsFBA6, leading to improved maintenance of ROS homeostasis and increased accumulation of sugars for osmoregulation, respectively (Yu et al, 2022). MsCML46 enhances tolerance to abiotic stresses through alleviating osmotic stress and oxidative damage in transgenic tobacco (Du et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Calmodulin and calmodulin-like gene family in barley: Identification, characterization and expression analyses

et al. 2022

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Calmodulin (CaM) and calmodulin-like (CML) proteins are Ca2+ relays and play diverse and multiple roles in plant growth, development and stress responses. However, CaM/CML gene family has not been identified in barley (Hordeum vulgare). In the present study, 5 HvCaMs and 80 HvCMLs were identified through a genome-wide analysis. All HvCaM proteins possessed 4 EF-hand motifs, whereas HvCMLs contained 1 to 4 EF-hand motifs. HvCaM2, HvCaM3 and HvCaM5 coded the same polypeptide although they differed in nucleotide sequence, which was identical to the polypeptides coded by OsCaM1-1, OsCaM1-2 and OsCaM1-3. HvCaMs/CMLs were unevenly distributed over barley 7 chromosomes, and could be phylogenetically classified into 8 groups. HvCaMs/CMLs differed in gene structure, cis-acting elements and tissue expression patterns. Segmental and tandem duplication were observed among HvCaMs/CMLs during evolution. HvCML16, HvCML18, HvCML50 and HvCML78 were dispensable genes and the others were core genes in barley pan-genome. In addition, 14 HvCaM/CML genes were selected to examine their responses to salt, osmotic and low potassium stresses by qRT-PCR, and their expression were stress-and time-dependent. These results facilitate our understanding and further functional identification of HvCaMs/CMLs.

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“…Some studies have shown that crops respond to salt stress by reducing the osmotic potential of cells and enhancing the water absorption capacity of roots by regulating inorganic ions 5 or synthesizing and accumulating organic substances 6 in cytoplasm, to alleviate the osmotic stress caused by salt. Besides, under salt stress, crops increase the activity of antioxidant enzymes 7 and the content of small molecular antioxidants (ascorbic acid, 8 glutathione, 9 tocopherol, 10 etc. ), to reduce the damage caused by excessive ROS, such as membrane damage, protein degradation, and enzyme inactivation, and alleviate the toxicity of soil salt.…”

Section: Introductionmentioning

confidence: 99%

Nitrogen application alleviates salt stress by enhancing osmotic balance, ROS scavenging, and photosynthesis of rapeseed seedlings (Brassica napus)

Tian

Wang

et al. 2022

Plant Signaling & Behavior

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Nitrogen application could alleviate salt stress on crops, but the specific physiological mechanism is still unclear. Therefore, in this study, a pot experiment was conducted to explore the effects of different application rates of nitrogen (0, 0.15, 0.30, and 0.45 g·kg −1 ) on the growth parameters, osmotic adjustment, reactive oxygen species scavenging, and photosynthesis of rapeseed seedlings planted in the soils with different concentrations of sodium chloride (1.5, 3.5, 5.5, and 7.5 g·kg −1 ). The results showed that nitrogen could alleviate the inhibition of salt on rapeseed growth, and improve the antioxidant enzyme activities and the contents of non-enzymatic substances, K + , soluble protein (SP), soluble sugar (SS), and proline. Besides, there was a significant correlation between the indexes of active oxygen scavenging system, osmoregulation system, and photosynthesis. Therefore, applying appropriate amount of nitrogen can promote the growth and development of rapeseed seedlings under salt stress, accelerate the scavenging of reactive oxygen species, maintain osmotic balance, and promote photosynthesis. This study will improve our understanding on the mechanism by which nitrogen application alleviates salt stress to crops.

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Alfalfa (Medicago sativa L.) MsCML46 gene encoding calmodulin-like protein confers tolerance to abiotic stress in tobacco

Cited by 22 publications

References 95 publications

Comparative Transcriptome Analysis Unravels Defense Pathways of Fraxinus velutina Torr Against Salt Stress

Comparative Transcriptome Analysis Unravels Defense Pathways of Fraxinus velutina Torr Against Salt Stress

Calmodulin and calmodulin-like gene family in barley: Identification, characterization and expression analyses

Nitrogen application alleviates salt stress by enhancing osmotic balance, ROS scavenging, and photosynthesis of rapeseed seedlings (Brassica napus)

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