Aleutian Mink Disease Virus in the breeding environment in Poland and its place in the global epidemiology of AMDV

Kowalczyk, Marek; Horecka, Beata; Jakubczak, Andrzej

doi:10.1016/j.virusres.2019.197665

Cited by 12 publications

(23 citation statements)

References 26 publications

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“…This farm introduced animals and farm supplies from other European countries in 2014. Therefore, one possible explanation for the introduction of AMDV would be international trade, a route that has also been suggested by other authors [ 15 16 24 ]. This hypothesis is supported by global phylogenetic analysis, where the sequences from this farm were related more to other contemporary sequences described in Poland, The Netherlands, and Greece ( Supplementary Figs.…”

Section: Discussionmentioning

confidence: 72%

“…Accordingly, the application of molecular epidemiology has proved useful for tracking possible sources of AMDV infections [ 11 22 ]. This has encouraged many producing countries to characterize their AMDV strains molecularly, and study their relationship with strains from different regions and countries [ 14 15 23 24 ]. On the other hand, available data on Spanish AMDV strains are extremely scarce, and they do not represent the whole Spanish mink sector.…”

Section: Introductionmentioning

confidence: 99%

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Molecular epidemiology of Aleutian mink disease virus causing outbreaks in mink farms from Southwestern Europe: a retrospective study from 2012 to 2019

et al. 2020

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Background Aleutian mink disease virus (AMDV) causes major economic losses in fur-bearing animal production. The control of most AMDV outbreaks is complex due to the difficulties of establishing the source of infection based only on the available on-farm epidemiological data. In this sense, phylogenetic analysis of the strains present in a farm may help elucidate the origin of the infection and improve the control and biosecurity measures. Objectives This study had the following aims: characterize the AMDV strains from most outbreaks produced at Spanish farms between 2012–2019 at the molecular level, and assess the utility of the combined use of molecular and epidemiological data to track the possible routes of infection. Methods Thirty-seven strains from 17 farms were partially sequenced for the NS1 and VP2 genes and analyzed phylogenetically with other strains described worldwide. Results Spanish AMDV strains are clustered in four major clades that generally show a good geographical correlation, confirming that most had been established in Spain a long time ago. The combined study of phylogenetic results and epidemiological information of each farm suggests that most of the AMDV outbreaks since 2012 had been produced by within-farm reservoirs, while a few of them may have been due to the introduction of the virus through international trade. Conclusions The combination of phylogenetic inference, together with epidemiological data, helps assess the possible origin of AMDV infections in mink farms and improving the control and prevention of this disease.

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Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of Aleutian mink disease virus causing outbreaks in mink farms from Southwestern Europe: a retrospective study from 2012 to 2019

et al. 2020

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“…There have been many reports on the possibility of using PCR in the diagnosis of viral diseases in mink [11,30]. Moreover, qPCR method can be used to analyse the sanitary state of the environment in terms of AMDV epidemiology [14,31,32]. While the qPCR reaction is more sensitive, it is diffi cult to introduce for routine diagnostics due to its relatively high cost.…”

Section: Discussionmentioning

confidence: 99%

“…Phylogenetic analysis was carried out in MEGA6. A phylogenetic tree was obtained by the Maximum Likelihood method with 1000 bootstrap replications for the sequence coding NS1 protein as this fragment has been used to analysis of relationships between isolates and tracing of outbreaks [13,14].…”

Section: Evaluation Of the Specifi City Of The Reactionmentioning

confidence: 99%

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Duplex PCR for Detection of Aleutian Disease Virus from Biological and Environmental Samples

2019

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Aleutian disease is one of the most serious disease entities affecting mink farms. The disease causes significant economic losses in mink breeding countries. The aim of the study was to optimize a diagnostic test based on duplex PCR to enable detection of Aleutian disease virus in biological and environmental samples. Blood (n = 40) and spleen (n = 40) samples from animals with suspected infection, and swabs from cages in which infected animals were kept (n = 20) were used for analysis. DNA was isolated from the samples, followed by optimization of the duplex PCR reaction targeting sequences coding NS1 and VP2 proteins. The qPCR method was used to determine the sensitivity of the reaction. The specificity of the analysis was confirmed by the sequencing results. Optimized duplex PCR enabled detection of Aleutian Mink Disease Virus (AMDV) genetic material in biological and environmental samples. Testing of the sensitivity of the method indicated clear amplification for both primer pairs at 102 copies of viral DNA in a reaction. Sequencing confirmed the specificity of the reaction, which in the case of both primer pairs indicated an over 90% agreement between the isolates and the variants of the virus from the databases. The use of duplex PCR to detect two regions of the AMDV genome may increase the sensitivity and specificity of the method and significantly expand the possibilities of further analysis based on sequencing.

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Genomic characterization and phylogenetic analysis of Aleutian mink disease virus identified in a sudden death mink case

Cui,

Yang,

Wang

et al. 2023

Comparative Immunology, Microbiology and Infectious Diseases

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Aleutian Mink Disease Virus in the breeding environment in Poland and its place in the global epidemiology of AMDV

Cited by 12 publications

References 26 publications

Molecular epidemiology of Aleutian mink disease virus causing outbreaks in mink farms from Southwestern Europe: a retrospective study from 2012 to 2019

Molecular epidemiology of Aleutian mink disease virus causing outbreaks in mink farms from Southwestern Europe: a retrospective study from 2012 to 2019

Duplex PCR for Detection of Aleutian Disease Virus from Biological and Environmental Samples

Genomic characterization and phylogenetic analysis of Aleutian mink disease virus identified in a sudden death mink case

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