Aldrin epoxidase from pea roots

Earl, JW; Kennedy, Ivan R.

doi:10.1016/0031-9422(75)85340-4

Cited by 22 publications

(4 citation statements)

References 16 publications

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“…Moreover, they are able to stimulate the activity of some enzymes, probably by removing metals that inhibit them. 16 These chelating ligands are also employed for several interesting environmental applications. The need to remove highly toxic compounds from potential sources of drinking water with efficient catalytic materials is of profound importance.…”

Section: Introductionmentioning

confidence: 99%

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Noncovalent Interactions of Cu⁺ with N-Donor Ligands (Pyridine, 4,4-Dipyridyl, 2,2-Dipyridyl, and 1,10-Phenanthroline): Collision-Induced Dissociation and Theoretical Studies

Rannulu

Rodgers

2007

J. Phys. Chem. A

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Collision-induced dissociation of complexes of Cu+ bound to a variety of N-donor ligands (N-L) with Xe is studied using guided ion beam tandem mass spectrometry. The N-L ligands examined include pyridine, 4,4-dipyridyl, 2,2-dipyridyl, and 1,10-phenanthroline. In all cases, the primary and lowest-energy dissociation channel observed corresponds to the endothermic loss of a single intact N-L ligand. Sequential dissociation of additional N-L ligands is observed at elevated energies for the pyridine and 4,4-dipyridyl complexes containing more than one ligand. Ligand exchange processes to produce Cu+Xe are also observed as minor reaction pathways in several systems. The primary cross section thresholds are interpreted to yield 0 and 298 K bond dissociation energies (BDEs) after accounting for the effects of multiple ion-neutral collisions, the kinetic and internal energy distributions of the reactants, and dissociation lifetimes. Density functional theory calculations at the B3LYP/6-31G* level are performed to obtain model structures, vibrational frequencies, and rotational constants for the neutral N-L ligands and the Cu+(N-L)x complexes. The relative stabilities of the various conformations of these N-L ligands and Cu+(N-L)x complexes as well as theoretical BDEs are determined from single point energy calculations at the B3LYP/6-311+G(2d,2p) level of theory using B3LYP/6-31G* optimized geometries. Excellent agreement between theory and experiment is observed for all complexes containing one or two N-L ligands, while theory systematically underestimates the strength of binding for complexes containing more than two N-L ligands. The ground-state structures of these complexes and the trends in the sequential BDEs are explained in terms of stabilization gained from sd-hybridization and repulsive ligand-ligand interactions. The nature of the binding interactions in the Cu+(N-L)x complexes are examined via natural bond orbital analyses.

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Section: Introductionmentioning

confidence: 99%

“…Their activities are usually a consequence of their ability to complex metal ions. Moreover, they are able to stimulate the activity of some enzymes, probably by removing metals that inhibit them …”

Section: Introductionmentioning

confidence: 99%

Noncovalent Interactions of Cu⁺ with N-Donor Ligands (Pyridine, 4,4-Dipyridyl, 2,2-Dipyridyl, and 1,10-Phenanthroline): Collision-Induced Dissociation and Theoretical Studies

Rannulu

Rodgers

2007

J. Phys. Chem. A

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“…The in vitro epoxidation of the organochlorine insecticide, aldrin, to dieldrin was reported by Lichtenstein and Corbett [132] to be mainly associated with the soluble fraction after high speed centrifugation of active pea root homogenates; however, other investigations [133][134][135][136] have localized this epoxidase activity in the particulate fractions of plant root homogenates. The failure of these enzyme preparations to epoxidize the structurally-related insecticide, heptachlor [132][133][134], suggested a narrow substrate specificity.…”

Section: Specific Metabolism In Plantsmentioning

confidence: 93%

“…PVP has the capability to preserve enzyme activity by preventing binding of naturally occurring phenolic compounds or their oxidized products to enzyme proteins [137,138], a common problem associated with the isolation of active enzyme preparations from plant tissues. Earl and Kennedy [136] overcame this problem in the isolation of a partially purified aldrin epoxidase from pea roots by using rapid anaerobic gel filtration.…”

Section: Specific Metabolism In Plantsmentioning

confidence: 99%

Plant Uptake, Transport and Metabolism

Morrison

Cohen

1980

The Handbook of Environmental Chemistry

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The biochemical mechanisms of the plant activation of promutagenic aromatic amines

Wagner

Verdier

Plewa

1990

Environ and Mol Mutagen

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Using specific monooxygenase and oxidase inhibitors in a plant cell/microbe coincubation assay, the biochemical mechanisms of the plant activation of two aromatic amines were compared. The biological endpoints included mutation induction, inhibition of mutagenicity, viability of the plant cells (activating system), and viability of the microbial cells (genetic indicator organism). The activation of m-phenylenediamine by TX1 cells was mediated by enzyme systems that were inhibited by diethyldithiocarbamate, potassium cyanide, methimazole, (+)-catechin or acetaminophen. The inhibition by metyrapone was attended by toxicity in the plant cells. These data implicate a TX1 cell peroxidase and a FAD-dependent monooxygenase in the plant activation of m-phenylenediamine. The TX1 cell activation of 2-aminofluorene was inhibited by diethyldithiocarbamate, 7,8-benzoflavone, acetaminophen or (+)-catechin. An additional pathway of the plant cells in the activation of 2-aminofluorene may involve a cytochrome P-448-type N-hydroxylase.

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Aldrin epoxidase from pea roots

Cited by 22 publications

References 16 publications

Noncovalent Interactions of Cu⁺ with N-Donor Ligands (Pyridine, 4,4-Dipyridyl, 2,2-Dipyridyl, and 1,10-Phenanthroline): Collision-Induced Dissociation and Theoretical Studies

Noncovalent Interactions of Cu⁺ with N-Donor Ligands (Pyridine, 4,4-Dipyridyl, 2,2-Dipyridyl, and 1,10-Phenanthroline): Collision-Induced Dissociation and Theoretical Studies

Plant Uptake, Transport and Metabolism

The biochemical mechanisms of the plant activation of promutagenic aromatic amines

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Aldrin epoxidase from pea roots

Cited by 22 publications

References 16 publications

Noncovalent Interactions of Cu+ with N-Donor Ligands (Pyridine, 4,4-Dipyridyl, 2,2-Dipyridyl, and 1,10-Phenanthroline): Collision-Induced Dissociation and Theoretical Studies

Noncovalent Interactions of Cu+ with N-Donor Ligands (Pyridine, 4,4-Dipyridyl, 2,2-Dipyridyl, and 1,10-Phenanthroline): Collision-Induced Dissociation and Theoretical Studies

Plant Uptake, Transport and Metabolism

The biochemical mechanisms of the plant activation of promutagenic aromatic amines

Contact Info

Product

Resources

About

Noncovalent Interactions of Cu⁺ with N-Donor Ligands (Pyridine, 4,4-Dipyridyl, 2,2-Dipyridyl, and 1,10-Phenanthroline): Collision-Induced Dissociation and Theoretical Studies

Noncovalent Interactions of Cu⁺ with N-Donor Ligands (Pyridine, 4,4-Dipyridyl, 2,2-Dipyridyl, and 1,10-Phenanthroline): Collision-Induced Dissociation and Theoretical Studies