Aldosterone in vitro restores nephron Na?K-ATPase of distal segments from adrenalectomized rabbits

Horster, Michael; Schmid, Heide; Schmidt, Udo

doi:10.1007/bf00584554

Cited by 56 publications

(20 citation statements)

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“…As reported earlier (17)(18)(19), short term aldosterone (3 h) increased the maximal hydrolytic activity of the Na,K-ATPase by ϳ200% (ADX control, 323 Ϯ 80; ADX aldosterone, 940 Ϯ 150 pmol ATP ϫ mm Ϫ1 ϫ h Ϫ1 ; n ϭ 9; p Ͻ 0.01). We performed Western blotting to test whether or not this increase in enzyme activity could be explained by an increase in the cellular pool of the Na,K-ATPase.…”

Section: Short Term Aldosterone Increases In Vivo Nak-atpase Hydrolysupporting

confidence: 79%

“…Concerning the effect of aldosterone on the Na,K-ATPase, measurements performed two decades ago on cortical collecting ducts (CCD) of adrenalectomized rats have shown that aldosterone restores within 1-3 h the Na,K-ATPase hydrolytic activity V max and/or the number of ouabain binding sites (17)(18)(19)(20)(21). The nature of this effect was not known because the data could not distinguish between the possibilities that additional Na ϩ pump units are synthesized, that Na ϩ pumps are activated in situ, or that Na ϩ pumps are recruited/translocated from an intracellular pool to the cell surface.…”

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confidence: 99%

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Short Term Effect of Aldosterone on Na,K-ATPase Cell Surface Expression in Kidney Collecting Duct Cells

Summa¹,

Mordasini²,

Roger³

et al. 2001

Journal of Biological Chemistry

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Aldosterone controls extracellular volume and blood pressure by regulating Na ؉ reabsorption, in particular by epithelia of the distal nephron. A main regulatory site of this transcellular transport is the epithelial sodium channel (ENaC) that mediates luminal Na ؉ influx. The Na,K-ATPase (Na ؉ pump) that coordinately extrudes Na ؉ across the basolateral membrane is known to be regulated by short term aldosterone as well. We now show that in the cortical collecting duct (CCD) from adrenalectomized rats, the increase in Na,K-ATPase activity (approximately 3-fold in 3 h), induced by a single aldosterone injection, can be fully accounted by the increase in Na,K-ATPase cell surface expression (؉ 497 ؎ 35%). The short term aldosterone action was further investigated in cultured mouse collecting duct principal cells mpkCCD cl4 . Within 2 h, maximal Na,K-ATPase function assessed by Na ؉ pump current (I p ) measurements and Na,K-ATPase cell surface expression were increased by 20 -50%. Aldosterone did not modify the Na ؉ dependence of the Na ؉ pumps and induced transcription-and translation-dependent actions on pump surface expression and current independently of ENaC-mediated Na ؉ influx. In summary, short term aldosterone directly increases the cell surface expression of preexisting Na ؉ pumps in kidney CCD target cells. Thus, aldosterone controls Na ؉ reabsorption in the short term not only by regulating the apical cell surface expression of ENaC (Loffing, J., Zecevic, M., Feraille, E., Kaissling, B., Asher, C., Rossier, B. C., Firestone, G. L., Pearce, D., and Verrey, F. (2001) Am. J. Physiol. 280, F675-F682) but also by coordinately acting on the basolateral cell surface expression of the Na,K-ATPase.

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Section: Short Term Aldosterone Increases In Vivo Nak-atpase Hydrolysupporting

confidence: 79%

mentioning

confidence: 99%

Short Term Effect of Aldosterone on Na,K-ATPase Cell Surface Expression in Kidney Collecting Duct Cells

Summa¹,

Mordasini²,

Roger³

et al. 2001

Journal of Biological Chemistry

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“…Adrenalectomy reduces the activity of Na,K-ATPase in both the cortical and medullary segments of the thick ascending limb (34)(35)(36)(37)(38)(39)(40)(41). Although early studies indicated that aldosterone was capable of increasing the activity of Na,K-ATPase after adrenalectomy (36,39,40), more recent studies have shown that dexamethasone but not aldosterone increases enzyme activity (34,35,38 (6) did not observe an effect of an aute infusion of aldosterone on potassium reabsorption in the loop of Henle. This latter observation contrasts with the results in the present study, in which chronic aldosterone treatment stimulated potassium reabsorption.…”

Section: Discussionmentioning

confidence: 99%

Regulation by adrenal corticosteroids of sodium and potassium transport in loop of Henle and distal tubule of rat kidney.

Stanton

1986

J. Clin. Invest.

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“…Horster and co-workers demonstrated that adrenalectomy caused a decrease in Na-K-ATPase in both thick ascending limb and collecting duct of rabbits (1). Aldosterone, when added in vitro to these nephron segments, restored Na-KATPase activity to normal within 1 h of incubation in both thick ascending limb and cortical collecting tubule.…”

Section: Introductionmentioning

confidence: 99%

Effect of adrenalectomy on transport in the rat medullary thick ascending limb.

Work¹,

Jamison²

1987

J. Clin. Invest.

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Previous studies in adrenalectomized (Adx) rats suggest that aldosterone may regulate ion transport in the ascending portion of Henle's loop. In order to examine directly the effect of adrenalectomy on transport, medullary thick ascending limb (Mtal) segments were isolated from Adx, Adx replaced with aldosterone (Adx + Ald, 0.5 &g* 100 g* body wt * d), and control Sprague-Dawley rats. Both net sodium and net chloride fluxes were significantly less in the Mtal segments from Adx rats compared with those in the control or Adx + Ald group. Physiologic levels of exogenous aldosterone increased net sodium chloride flux toward control values in the Adx + Ald group. Net potassium flux was not different among the three groups. We conclude that adrenalectomy impairs reabsorptive NaCl but not K transport in the Mtal, and that aldosterone restores this process. This reabsorptive defect may contribute to the urinary concentrating and diluting abnormality associated with adrenal insufficiency.

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Aldosterone in vitro restores nephron Na?K-ATPase of distal segments from adrenalectomized rabbits

Cited by 56 publications

References 11 publications

Short Term Effect of Aldosterone on Na,K-ATPase Cell Surface Expression in Kidney Collecting Duct Cells

Short Term Effect of Aldosterone on Na,K-ATPase Cell Surface Expression in Kidney Collecting Duct Cells

Regulation by adrenal corticosteroids of sodium and potassium transport in loop of Henle and distal tubule of rat kidney.

Effect of adrenalectomy on transport in the rat medullary thick ascending limb.

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