Aldosterone: From biosynthesis to non-genomic action onto the proteome

Zöllner, Susanne; Hwang, Kyung Hoon; Wilzewski, Britta; Carapito, Christine; Leize‐Wagner, Emmanuelle; Dorsselaer, Alain Van; Bernhardt, Rita

doi:10.1016/j.steroids.2007.12.029

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…These effects are not mediated by gene expression but appear to involve activation of different signaling pathways including PKC, phosphoinositide 3-kinase, and c-Src and of transporters including the Na ϩ /H ϩ exchanger, the Na-K-2Cl cotransporter, and a Mg 2ϩ transporter transient receptor potential melastatin 7 cation channel (TRPM7) (216,582,595). The nongenomic effects are thought to be mediated by interaction with MR or other receptors (including G protein-coupled estrogen receptor 1, GPER1) which are located in the cell membrane (unlike the classical cytosolic/nuclear receptors) (101).…”

Section: B Nonepithelial and Nongenomic Actionsmentioning

confidence: 99%

Primary Aldosteronism: Changing Definitions and New Concepts of Physiology and Pathophysiology Both Inside and Outside the Kidney

Stowasser

Gordon

2016

Physiological Reviews

127

134

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In the 60 years that have passed since the discovery of the mineralocorticoid hormone aldosterone, much has been learned about its synthesis (both adrenal and extraadrenal), regulation (by renin-angiotensin II, potassium, adrenocorticotrophin, and other factors), and effects (on both epithelial and nonepithelial tissues). Once thought to be rare, primary aldosteronism (PA, in which aldosterone secretion by the adrenal is excessive and autonomous of its principal regulator, angiotensin II) is now known to be the most common specifically treatable and potentially curable form of hypertension, with most patients lacking the clinical feature of hypokalemia, the presence of which was previously considered to be necessary to warrant further efforts towards confirming a diagnosis of PA. This, and the appreciation that aldosterone excess leads to adverse cardiovascular, renal, central nervous, and psychological effects, that are at least partly independent of its effects on blood pressure, have had a profound influence on raising clinical and research interest in PA. Such research on patients with PA has, in turn, furthered knowledge regarding aldosterone synthesis, regulation, and effects. This review summarizes current progress in our understanding of the physiology of aldosterone, and towards defining the causes (including genetic bases), epidemiology, outcomes, and clinical approaches to diagnostic workup (including screening, diagnostic confirmation, and subtype differentiation) and treatment of PA.

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Section: B Nonepithelial and Nongenomic Actionsmentioning

confidence: 99%

Primary Aldosteronism: Changing Definitions and New Concepts of Physiology and Pathophysiology Both Inside and Outside the Kidney

Stowasser

Gordon

2016

Physiological Reviews

127

134

View full text Add to dashboard Cite

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“…Linking CTS with aldosterone action, it has also been shown that both share common intracellular signaling pathways [20,21]. …”

Section: Introductionmentioning

confidence: 99%

Influence of Aldosterone and Salt or Ouabain in A10 Rat Aorta Smooth Muscle Cells

Schwerdt

Frisch²,

Mildenberger³

et al. 2012

J Vasc Res

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Background/Aims: It is currently under debate whether aldosterone is able to induce fibrosis or whether it acts only as a cofactor under pathological conditions, e.g. as an elevated salt (NaCl) load. Methods: We tested the interaction of 10 nM aldosterone, 15 mM NaCl and 1 µM ouabain using rat aorta smooth muscle cells (A10) with respect to the following parameters: necrosis, apoptosis, glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase activity, glutathione (GSH) content, collagen and fibronectin homeostasis and intracellular calcium distribution. Results: Necrosis rates were increased after 48 h of incubation with aldosterone, salt or ouabain and in the combination of aldosterone and salt or ouabain. Apoptosis rates were decreased. A reduced defense capacity against oxidative stress was mirrored in the decreased G6PD activity and GSH content. Collagen III or fibronectin synthesis rates were unchanged, but gelatinase activity was increased resulting in a decreased media collagen III and fibronectin content. Calcium stores were increased by aldosterone in combination with ouabain. Conclusion: Aldosterone and salt per se can lead to cell injury that is aggravated in combination or with cardiotonic steroids. In cooperation with other vascular cells, this can generate a permissive milieu enabling aldosterone or salt to promote more extensive vascular injury.

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“…The applicability of the protocol for eukaryotic proteomes was proven using a simple eukaryotic organism, the fission yeast S. pombe. This selection furthermore allowed us to complement the proteomic studies on this model organism, previously reported by us [1,18,19]. After proving the general applicability to eukaryotic proteomes, we set the focus of the study onto human hepatocytes.…”

Section: Introductionmentioning

confidence: 99%

Shotgun proteomic analysis of the microsomal fraction of eukaryotic cells using a two‐dimensional reversed‐phase×ion‐pair reversed‐phase HPLC setup

Wörner

Melchior

Delmotte

et al. 2009

J of Separation Science

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A RPxIP-RP HPLC separation scheme was combined with on-line ESI-IT tandem MS or off-line MALDI tandem TOF MS and applied to the analysis of eukaryotic subcellular proteomes. Previous proteomic studies [1] were complemented by the approval of the approach to eukaryotic proteomes using the fission yeast Schizosaccharomyces pombe. The major focus was set to the analysis of primary human hepatocyte microsomes, representing a compartment of high interest due to its involvement in xenobiotic detoxification and cholesterol homeostasis. Of the 588 proteins identified from two donors, 24% are involved in cholesterol homeostasis or xenobiotic/lipid metabolism. Up to 50% of the identified proteins belong to the group of membrane proteins, difficult to investigate using gel-based proteomic approaches. We further demonstrated the reproducibility and comparability of the approach and reduced the amount of sample load by almost 70% with only minor loss of information about the proteins identified in the samples. The presented study clearly demonstrates the good applicability of the experimental setup to the analysis of subcellular proteomes including large membrane fractions, where only low amounts of sample material are available.

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Aldosterone: From biosynthesis to non-genomic action onto the proteome

Cited by 8 publications

References 37 publications

Primary Aldosteronism: Changing Definitions and New Concepts of Physiology and Pathophysiology Both Inside and Outside the Kidney

Primary Aldosteronism: Changing Definitions and New Concepts of Physiology and Pathophysiology Both Inside and Outside the Kidney

Influence of Aldosterone and Salt or Ouabain in A10 Rat Aorta Smooth Muscle Cells

Shotgun proteomic analysis of the microsomal fraction of eukaryotic cells using a two‐dimensional reversed‐phase×ion‐pair reversed‐phase HPLC setup

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