Aldolase C is a novel molecular marker for folliculo-stellate cells in rodent pituitary

Fujiwara, Ken; Tsukada, Takehiro; Horiguchi, Kotaro; Fujiwara, Yoko; Takemoto, Konomi; Nio‐Kobayashi, Junko; Ohno, Nobuhiko; Inoue, Kinji

doi:10.1007/s00441-020-03200-1

Cited by 13 publications

(14 citation statements)

References 43 publications

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“…FSCs and pituicytes both expressed S100b, historically viewed as a definitive marker for FSCs in the rat anterior pituitary (Le Tissier & Mollard, 2021). Both cell types also expressed aldolase, fructosebisphosphate C (Aldoc, Figure 1d), and a recently reported marker for FSCs in both mouse and rat (Fujiwara et al, 2020). Notably, S100b and Aldoc are also markers of astrocytes (Verkhratsky & Nedergaard, 2018).…”

Section: The Resident Cell Types Of the Pituitary Glandmentioning

confidence: 84%

The astroglial and stem cell functions of adult rat folliculostellate cells

Fletcher

Smiljanić

Prévide

et al. 2022

Glia

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The mammalian pituitary gland is a complex organ consisting of hormone-producing cells (HPC), nonhormonal folliculostellate cells (FSC) and pituicytes, vascular pericytes and endothelial cells, and putative Sox2-expressing stem cells. Here, we used scRNAseq analysis of adult female rat pituitary cells to study the heterogeneity of pituitary cells with a focus on evaluating the transcriptomic profile of the Sox2expressing population. Samples containing whole pituitary and separated anterior and posterior lobe cells allowed the identification of all expected pituitary resident cell types and lobe-specific subpopulations of vascular cells. Sox2 was expressed uniformly in all FSC, pituicytes, and a fraction of HPC. FSC comprised two subclusters; FSC1 contained more cells but expressed less genetic diversity compared to FSC2. The latter contained proliferative cells, expressed genes consistent with stem cell niche formation, including tight junctions, and shared genes with HPC. The FSC2 transcriptome profile was also consistent with the activity of pathways regulating cell proliferation and stem cell pluripotency, including the Hippo and Wnt pathways. The expression of other stem cell marker genes was common for FSC and pituicytes (Sox9,

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Section: The Resident Cell Types Of the Pituitary Glandmentioning

confidence: 84%

The astroglial and stem cell functions of adult rat folliculostellate cells

Fletcher

Smiljanić

Prévide

et al. 2022

Glia

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“…More recently, specific pituitary expression of aldolase C in FS cells has been described in mice, which may allow increased genetic manipulation in this model species. 18 Dispersed FS cells in culture readily self-organise into aggregates of cells that resemble those of the intact pituitary and, in mixed cultures with other pituitary cell types, form clusters with hormone producing cells. 19 A role for the chemoattractant molecule CXCL12 and its receptor CXCR4, both expressed by FS cells, has been described in this in vitro recapitulation of cell organisation.…”

Section: Fs Cell S and Their Cell Identit Ymentioning

confidence: 99%

Renewing an old interest: Pituitary folliculostellate cells

Tissier

Mollard

2021

J Neuroendocrinology

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Increasingly, neuroendocrine research focuses on its potential for an impact of novel findings on highly-prevalent human health problems, in particular those where the underlying physiology and its dysfunction is poorly understood. Research on the pituitary, the master gland relaying hormonal information between the brain and many peripheral tissues, cannot escape this trend, as exemplified by the growing hope of regenerative medicine with the recent discovery of adult stem cells in the pituitary parenchyma. One exciting aspect of stem cell therapy is its potential to correct a number of pituitary disorders with aetiologies that are poorly understood. This focus on stem cells and their potential may explain how a subpopulation of pituitary cells, devoid of secretory granules, has changed from being described as folliculostellate (FS) cells (coined by Evelyne Vila-Porcile in 1972 1 ) to adult Sox2+ve stem cells. Recent studies have highlighted an important aspect of these stem cells, in that they are the

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“…Folliculo-stellate (FS) cells are specialised AL glial cells (14). These express S100b in the rat, but not in the mouse ((15, 16), Sup. Fig.2).…”

Section: Resultsmentioning

confidence: 99%

“…Fig.2). To clarify their relationship with SCs, we examined expression of Aldolase C (16) and of Slc152a , which encodes a transporter responsible for the FS specific import of AMCA (17). Both are selectively present in SCs, in clusters 0 and 2, implying that in the mouse FS and SCs are overlapping populations.…”

Section: Resultsmentioning

confidence: 99%

SOX9-positive pituitary stem cells differ according to their position in the gland and maintenance of their progeny depends on context

Rizzoti

Chakravarty

Sheridan

et al. 2022

Preprint

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Stem cell (SC) differentiation and maintenance of the resultant nascent cells underlie regeneration in many organs, but it is often difficult to pinpoint contribution of either process to SC output. In the pituitary gland, a central regulator of endocrine axes, a population of adult SCs undergo activation following target organ ablation in the mouse, providing a well-characterized paradigm to study regeneration in a multi-organ system. Here we have used single cell technologies to characterize both heterogeneity of this population and modalities of regeneration. We consequently performed short and long-term lineage tracing experiments based on our transcriptomic analyses predictions. Our result show that SC differentiation occurs more frequently than previously thought. In regenerative conditions, differentiation is both increased and more diverse than lineage tracing experiments had formerly demonstrated. Examination of SC progeny at different time points demonstrate that, while differentiation is indeed relatively frequent, maintenance of the nascent cells is an important regulatory step controlling SC-derived regeneration, reconciliating our data with previous observations and highlighting a trophic role for the microenvironment. Analyses of cell trajectories and gene regulatory networks (GRNs) further predict modalities, and identifies potential new players involved in regeneration. Our model provides a unique system to study the influence of evolving physiological states on the mechanisms of regeneration from SCs.

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Aldolase C is a novel molecular marker for folliculo-stellate cells in rodent pituitary

Cited by 13 publications

References 43 publications

The astroglial and stem cell functions of adult rat folliculostellate cells

The astroglial and stem cell functions of adult rat folliculostellate cells

Renewing an old interest: Pituitary folliculostellate cells

SOX9-positive pituitary stem cells differ according to their position in the gland and maintenance of their progeny depends on context

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