Aldolase activities of the small intestinal mucosa in malabsorption states and hereditary fructose intolerance

Streb, Hanspeter; Posselt, Hans-Georg; Wolter, Karl E.; Bender, S. W.

doi:10.1007/bf00441161

Cited by 2 publications

(3 citation statements)

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“…Key Words: Aldola se-Crypt hyperplastic villous atrophy-Malabsorptive dise ases. can be established by inte stinal biop sy (5). Typical acti vity patterns were also ob ser ved in cr ypt hyperpl astic villous atrophy.…”

mentioning

confidence: 80%

“…Further det ails con cerning definit ion of disea se entities , handling of biop sy mat erial, and biochemical analysis have been outlined previously (5). Thi s communication pr imaril y int ends to emphasiz e a close correlation between fructose-I,6-p/fructose-I-p activity ratio and morphologic abnormalitie s quantified by morphometry.…”

Section: Methodsmentioning

confidence: 99%

“…3, N o 4,. 1984 crypt I vi llo us height tose-1,6-d iphosphate (aldolase A) was markedly increased(5). The correlation of these biochemical data with morphology shows that the aldolase A/B activity ratio allows precise biochemical quantification of a damaged mucosa with cryp t hyperplastic villous atro phy.…”

mentioning

confidence: 99%

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Biochemical Quantification of Crypt Hyperplastic Villous Atrophy by Aldolase Activity Assay

Bender,

Posselt,

Stäps

et al. 1984

J. pediatr. gastroenterol. nutr.

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Aldolase activity with the two substrates fructose‐1‐phosphate and fructose‐1,6‐diphosphate was measured in the homogenate of small intestinal biopsy specimens from children with different malabsorptive diseases (celiac disease, cow's milk protein intolerance, infectious diarrhea, giardiasis, and Crohn's disease) and controls. It is demonstrated that the ratio of fructose‐1,6− diphosphate/fructose‐1‐phosphate activity, which reflects the relative amounts of the crypt enzyme aldolase A (EC 4.1.2.13) and the villous enzyme aldolase B (EC 4.1.2.7), correlates very well with both the ratio of crypt to villous height (correlation factor r = 0.92) and the mitotic index (r = 0.80).

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mentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%