“…In the 2-thiobarbituric acid (TBA) assay MDA, a secondary decomposition product of polyunsaturated fatty acids with three or more double bonds, reacts with TBA to form a stable pink chromophore (Dahle, Hill, & Holman, 1962). The term TBA-reactive substances (TBARS) is more commonly used to describe the assay because the term takes into account the contribution of compounds other than MDA, such as 2,4-heptadienal, t-2-heptenal, t-2-hexenal and hexanal, to chromophore formation following reaction with TBA (Sun, Faustman, Senecal, Wilkinson, & Furr, 2001).…”