Aldehyde dehydrogenase activity helps identify a subpopulation of murine adipose-derived stem cells with enhanced adipogenic and osteogenic differentiation potential

Itoh, Harumichi; Nishikawa, Shimpei; Haraguchi, Tomoya; Arikawa, Yu; Eto, Shotaro; Hiyama, Masato; Iseri, Toshie; Itoh, Yoshiki; Nakaichi, Munekazu; Sakai, Yusuke; Tani, Kenji; Taura, Yasuho; Itamoto, Kazuhito

doi:10.4252/wjsc.v9.i10.179

Cited by 8 publications

(5 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study contains the results confirming the expression of intracellular markers vimentin, desmin, αSMA, and the activity of aldehyde dehydrogenase (ALDH). The level of ALDH is currently used as a selection marker of the stem cells due to its relation to self-renewal and differentiation abilities [ 54 , 55 ]. The phenotypic profile was confirmed by flow cytometry and PCR methods on the mRNA level.…”

Section: Discussionmentioning

confidence: 99%

Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells

Tirpáková

Vašíček

Svoradová

et al. 2021

Genes

View full text Add to dashboard Cite

Although the rabbit is a frequently used biological model, the phenotype of rabbit adipose-derived mesenchymal stem cells (rAT-MSCs) is not well characterized. One of the reasons is the absence of specific anti-rabbit antibodies. The study aimed to characterize rAT-MSCs using flow cytometry and PCR methods, especially digital droplet PCR, which confirmed the expression of selected markers at the mRNA level. A combination of these methods validated the expression of MSCs markers (CD29, CD44, CD73, CD90 and CD105). In addition, cells were also positive for CD49f, vimentin, desmin, α-SMA, ALDH and also for the pluripotent markers: NANOG, OCT4 and SOX2. Moreover, the present study proved the ability of rAT-MSCs to differentiate into a neurogenic lineage based on the confirmed expression of neuronal markers ENO2 and MAP2. Obtained results suggest that rAT-MSCs have, despite the slight differences in marker expression, the similar phenotype as human AT-MSCs and possess the neurodifferentiation ability. Accordingly, rAT-MSCs should be subjected to further studies with potential application in veterinary medicine but also, in case of their cryopreservation, as a source of genetic information of endangered species stored in the gene bank.

show abstract

Section: Discussionmentioning

confidence: 99%

Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells

Tirpáková

Vašíček

Svoradová

et al. 2021

Genes

View full text Add to dashboard Cite

show abstract

“…In addition, Harumichi et al [ 72 ] reported that although significant differences in proliferation capacity were not seen, the adipogenic and osteogenic differentiation capacities were higher in aldehyde dehydrogenase (ALDH)-high subpopulations than in ALDH-low subpopulations. All these studies revealed that osteogenic potential is related to different subpopulations.…”

Section: Advances In Related Basic Researchmentioning

confidence: 99%

Stem cell therapy for treating osteonecrosis of the femoral head: From clinical applications to related basic research

Lin

Liang

et al. 2018

Stem Cell Res Ther

View full text Add to dashboard Cite

Osteonecrosis of the femoral head (ONFH) is a refractory disease that is associated with collapse of the femoral head, with a risk of hip arthroplasty in younger populations. Thus, there has been an increased focus on early interventions for ONFH that aim to preserve the native articulation. Stem cell therapy is a promising treatment, and an increasing number of recent studies have focused on this topic. Many clinical studies have reported positive outcomes of stem cell therapy for the treatment of ONFH. To improve the therapeutic effects of this approach, many related basic research studies have also been performed. However, some issues must be further explored, such as the appropriate patient selection procedure, the optimal stem cell selection protocol, the ideal injection number, and the safety of stem cell therapy. The purpose of this review is to summarize the available clinical studies and basic research related to stem cell therapy for ONFH.

show abstract

“…Although ALDH br cells with stem cell properties have been detected in various normal tissues, including the bone marrow [ 14 , 23 ], umbilical cord blood [ 24 , 25 ], mammary glands [ 26 , 27 ], heart [ 28 ], and adipose tissue [ 29 ], little is known about lung-resident ALDH br cells. A study showed that isolated murine airway basal and submucosal gland duct ALDH br cells exhibited stem cell properties in normal/healthy lungs [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Antifibrotic effect of lung-resident progenitor cells with high aldehyde dehydrogenase activity

et al. 2021

View full text Add to dashboard Cite

Background Aldehyde dehydrogenase (ALDH) is highly expressed in stem/progenitor cells in various tissues, and cell populations with high ALDH activity (ALDHbr) are associated with tissue repair. However, little is known about lung-resident ALDHbr. This study was performed to clarify the characteristics of lung-resident ALDHbr cells and to evaluate their possible use as a tool for cell therapy using a mouse model of bleomycin-induced pulmonary fibrosis. Methods The characteristics of lung-resident/nonhematopoietic (CD45−) ALDHbr cells were assessed in control C57BL/6 mice. The kinetics and the potential usage of CD45−/ALDHbr for cell therapy were investigated in bleomycin-induced pulmonary fibrosis. Localization of transferred CD45−/ALDHbr cells was determined using mCherry-expressing mice as donors. The effects of aging on ALDH expression were also assessed using aged mice. Results Lung CD45−/ALDHbr showed higher proliferative and colony-forming potential than cell populations with low ALDH activity. The CD45−/ALDHbr cell population, and especially its CD45−/ALDHbr/PDGFRα+ subpopulation, was significantly reduced in the lung during bleomycin-induced pulmonary fibrosis. Furthermore, mRNA expression of ALDH isoforms was significantly reduced in the fibrotic lung. When transferred in vivo into bleomycin-pretreated mice, CD45−/ALDHbr cells reached the site of injury, ameliorated pulmonary fibrosis, recovered the reduced expression of ALDH mRNA, and prolonged survival, which was associated with the upregulation of the retinol-metabolizing pathway and the suppression of profibrotic cytokines. The reduction in CD45−/ALDHbr/PDGFRα+ population was more remarkable in aged mice than in young mice. Conclusions Our results strongly suggest that the lung expression of ALDH and lung-resident CD45−/ALDHbr cells are involved in pulmonary fibrosis. The current study signified the possibility that CD45−/ALDHbr cells could find application as novel and useful cell therapy tools in pulmonary fibrosis treatment.

show abstract

Aldehyde dehydrogenase activity helps identify a subpopulation of murine adipose-derived stem cells with enhanced adipogenic and osteogenic differentiation potential

Cited by 8 publications

References 21 publications

Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells

Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells

Stem cell therapy for treating osteonecrosis of the femoral head: From clinical applications to related basic research

Antifibrotic effect of lung-resident progenitor cells with high aldehyde dehydrogenase activity

Contact Info

Product

Resources

About