Aldehyde dehydrogenase 1, a functional marker for identifying cancer stem cells in human nasopharyngeal carcinoma

Wu, Aibing; Luo, Weiren; Zhang, Qianbing; Yang, Zhijian; Zhang, Gong; Li, Siyi; Yao, Kangde

doi:10.1016/j.canlet.2012.11.046

Cited by 71 publications

(55 citation statements)

References 40 publications

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“…ALDH1 is an intracellular enzyme that oxidizes several kinds of aldehyde to carboxylic acid and shows strong activity in hematopoietic progenitor cells (Kastan et al 1990;Vasiliou et al 2004). In solid tumors, ALDH1 expression has been reported to be a poor prognostic factor in patients with rectal cancer, breast cancer, ovarian cancer, and nasopharyngeal cancer (Ginestier et al 2007;Huang et al 2009;Liu et al 2013;Wu et al 2013;Zhou et al 2010). Among seven putative CSLC markers examined in this study, a multivariate analysis showed that the positive expression of ALDH1 was significantly associated with unfavorable recurrence-free and overall survival after surgical resection of HGNEC.…”

Section: Discussionmentioning

confidence: 97%

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Clinicopathological significance of cancer stem-like cell markers in high-grade neuroendocrine carcinoma of the lung

Morise

Hishida

Takahashi

et al. 2015

J Cancer Res Clin Oncol

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Section: Discussionmentioning

confidence: 97%

“…To achieve a cure, these tumorigenic stem-like cells must be effectively targeted (Reya et al 2001). Studies have recently been conducted in several solid tumors to identify CSLCs as therapeutic targets, based on strategies that identify possible CSLCs using specific CSLC markers (Al-Hajj et al 2003;Huang et al 2009;Wu et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Clinicopathological significance of cancer stem-like cell markers in high-grade neuroendocrine carcinoma of the lung

Morise

Hishida

Takahashi

et al. 2015

J Cancer Res Clin Oncol

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“…We hypothesize that culturing the SP and NSP cells in complete medium after sorting may have caused the SP cells to differentiate, subsequently losing their stem cell properties. Previous studies have revealed that normal and neoplastic stem cells obtained from neural and epithelial organs only exhibit initial tumor-specific properties when cultured in serum-free medium containing epidermal growth factor (EGF) and fibroblast growth factor (FGF)-2 (33)(34)(35). In addition, adherent cells expanded in Laminin-coated culture plates in serum free medium containing N2-supplement, EGF and basic FGF maintain initial tumor-specific properties (36).…”

Section: Discussionmentioning

confidence: 99%

Dysregulation of the PI3K/Akt signaling pathway affects cell cycle and apoptosis of side population cells in nasopharyngeal carcinoma

et al. 2015

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Abstract.Increasing evidence has suggested that certain types of cancer possess their own stem-like cells, and that one subset of these cells, termed the side population (SP), may have an important role in tumorigenesis and cancer therapy. However, the molecular mechanisms underlying the modulation of SP cells in nasopharyngeal carcinoma (NPC) have remained elusive. In the present study, it was hypothesized that dysregulation of the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/Akt signaling pathway may influence SP and non-SP (NSP) phenotype. SP cells from the HK-1 NPC cell line were identified, and cancer stem cell markers were found to be highly expressed in SP cells compared with that of NSP cells. Freshly sorted SP cells demonstrated a significant increase in the proportion of cells in G0/G1 phase, while the majority of NSP cells were in the proliferative phase. Following 48 h of culture subsequent to cell sorting, the differences in cell cycle distribution between the SP and NSP cells converged. In addition, the apoptotic ratio of NSP cells was higher than that of SP cells at 24 h following sorting, but had no significant differences 48 h following sorting. To elucidate the potential mechanism mediating the cell cycle and apoptosis in SP cells, the expression levels of key molecules in the PI3K/Akt signaling pathway were evaluated. PI3K and Akt were upregulated, while 14-3-3σ protein was downregulated in SP cells when freshly sorted (0 h). However, there was no significant difference in the expression of these molecules between SP and NSP cells following 48 h of culture. These results suggested that dysregulation of the PI3K/Akt signaling pathway may be associated with the cell cycle and apoptosis of SP cells in NPC. However, further investigation is required to elucidate the detailed mechanisms underlying these effects. IntroductionIncreasing evidence has suggested that specific types of cancer may contain their own stem-like cells, known as cancer stem cells (CSCs), which have key roles in the initiation, maintenance and recurrence of tumors (1-3). In particular, attention has been paid to a subset of CSCs, termed the side population (SP), which was identified by flow cytometry. These SP cells are able to exclude the DNA binding dye, Hoechst 33342, and are highly enriched for stem cells in numerous types of tissue (4-6). SP cells have been isolated from multiple solid tumors, and studies have suggested that they may have significant roles in tumorigenesis and cancer therapy. A SP of cells in nasopharyngeal carcinoma (NPC) were found to exhibit characteristics of stem-like cancer cells (7-12). However, the molecular mechanisms underlying the modulation of these stem-like cell populations in NPC have remained elusive. Cellular proliferation is a critical process underlying the growth, development and regeneration of eukaryotic organisms, and appropriate control of the cell cycle is required for the proliferation of normal cells (13,14). Deregulation of the cell cycle is responsible for the aberrant...

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“…It has also been suggested that CD24 could be considered as a marker for nasopharyngeal CSCs, since CD24 + cells from NPC cell lines expressed SC genes and were highly proliferative, tumorigenic, and drug resistant [71]. The other introduced molecule for NPC-SCs is ALDH1, because cells with ALDH1 activity expressed pluripotent markers and presented great proliferation, differentiation, migration, and tumorigenic abilities [65,72,73]. Moreover, clinical studies indicated that ALDH1 expression was associated with aggressive tumor behavior and poor survival of NPC [74].…”

Section: Pharynxmentioning

confidence: 98%

Cancer stem cells in human digestive tract malignancies

2015

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Digestive tract malignancies, including oral, pharyngeal, esophageal, gastric, and colorectal cancers, are among the top 10 most common cancers worldwide. In spite of using various treatment modalities, cancer patients still suffer from recurrence and metastasis of malignant cells. Cancer stem cells (CSCs) are undifferentiated and highly proliferative malignant cells with unique properties mediated by overexpression of stemness markers, metastasis-related proteins, drug transporters, and DNA repair machinery. Due to their salient characteristics, it has been suggested that CSCs are responsible for tumor initiation, progression, invasion, recurrence, and therapy resistance. Exploring different aspects of CSC biology has fueled a great enthusiasm in designing novel therapeutic strategies to help patients. For instance, identification of markers associated with digestive tract CSCs, such as CD44, CD133, CD24, EpCAM, LGR5, ALDH1, and BMI1, has made it possible to develop more accurate diagnosis approaches. In addition, specifically targeting CSCs by their markers imposes fewer side effects and improves therapeutic outcomes. Here, we focus on the current status of CSC biology in digestive tract cancers, with emphasis on CSC markers, and review achieved progress in eradication of digestive tract CSC cells.

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Aldehyde dehydrogenase 1, a functional marker for identifying cancer stem cells in human nasopharyngeal carcinoma

Cited by 71 publications

References 40 publications

Clinicopathological significance of cancer stem-like cell markers in high-grade neuroendocrine carcinoma of the lung

Clinicopathological significance of cancer stem-like cell markers in high-grade neuroendocrine carcinoma of the lung

Dysregulation of the PI3K/Akt signaling pathway affects cell cycle and apoptosis of side population cells in nasopharyngeal carcinoma

Cancer stem cells in human digestive tract malignancies

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