Alcoholic Carbowax Prefixation and Formal Alcohol Fixation

Tang, Cang-Sing; Tang, Cecilia M.C.; Lau, Yee-yee; Kung, I. T. M.

doi:10.1159/000332843

Cited by 5 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to address this problem, coating fixatives were introduced, which provide a thin protective coating over cells. Coating fixatives include polyethylene glycol (Carbowax TM ; Dow Chemicals, Pittsburg, CA, USA) in addition to alcohol, which results in a reduction of artefacts and improved morphological preservation 26 . By itself, the addition of polyethylene glycol to fixative does not appear to impact DNA recovery, as amplification from cellular material fixed in either alcohol or alcohol/polyethylene glycol solution is highly efficient 12,27,28 .…”

Section: Discussionmentioning

confidence: 99%

Factors predicting successful DNA recovery from archival cervical smear samples

Canfell¹,

Gray

Snijders

et al. 2004

Cytopathology

View full text Add to dashboard Cite

Polymerase chain reaction (PCR)-based DNA testing of archival cervical smear slides is a useful method of retrospectively establishing the presence of the human papillomavirus (HPV) in cervical cells. A cellular DNA recovery test is performed in parallel to HPV DNA testing to ensure that sufficient cells are present and purification of sample DNA has been successfully performed. Previous studies have not comprehensively assessed DNA recovery rates in slides older than 13 years. We undertook a study to determine the factors impacting DNA recovery in 436 UK slides dating from 11 to 33 years prior to testing. Overall, a low cellular DNA recovery success rate of 29% was obtained but a strong trend was observed with increasing recovery rates the older the slides (P < 0.001). Recovery rates increased from 22% in the most recent slides collected from 1988 to 1992, to 61% in the oldest slides, collected in 1970-72. It is likely that fixation compounds incorporating acetic acid, introduced in the UK through the 1980s, have compromised subsequent attempts at PCR amplification. These findings emphasize the importance of the original fixation method in the success of DNA recovery from archival smear samples.

show abstract

Section: Discussionmentioning

confidence: 99%

Factors predicting successful DNA recovery from archival cervical smear samples

Canfell¹,

Gray

Snijders

et al. 2004

Cytopathology

View full text Add to dashboard Cite

show abstract

“…Saccomanno et al 21 recommend use of a solution of 2% polyethylene glycol (Carbowax 1540) in alcohol, which prevents shrinkage of sputum cells and ensures their good adhesion to glass slides. 23 We investigated the value of alcohol-Carbowax (Nakalai Tesque, Kyoto, Japan) solution to improve TUNEL reactivity. Our goal was to establish a reliable protocol that can be adapted to the TUNEL assay of cytologic preparations.…”

Section: Objective: To Determine the Optimal Fixation Methods For Cultmentioning

confidence: 99%

Optimal Prefixation of Cells To Demonstrate Apoptosis by the TUNEL Method

Kiyozuka¹,

Akamatsu²,

Singh³

et al. 1999

Acta Cytologica

View full text Add to dashboard Cite

“…Samples were centrifuged at 500g for 10 minutes. The sediment was treated with alcoholic carbowax (3 g carbowax in 100 mL 60% ethanol) as a prefixative 18 and 2% paraformaldehyde as a final fixative. Two slides precoated with 3-aminopropyltriethoxysilane were made from each specimen.…”

Section: Preparation Of Urinary Sedimentmentioning

confidence: 99%

Loss of Chromosome 16 From Renal Epithelial Cells in Humans

Gardner¹,

Yang²,

Skurnick³

et al. 2002

Hypertension

View full text Add to dashboard Cite

Abstract-This work explores the notion that low-frequency, acquired aneuploidy may play a role in complex genetic traits such as essential hypertension. To this end, renal epithelial cells in urinary sediments and in renal cysts were examined by fluorescent in situ hybridization with DNA probes specific for the heterochromatic and centromere regions of chromosomes 16 and 1. Chromosome 16 was probed because it harbors variant genes causing monogenic hypertension. These genes have also been investigated for their role in essential hypertension. Chromosome 1 was also probed as an internal control. Higher proportions of renal epithelial cells in the urinary sediments showed monosomy of chromosome 16 than monosomy of chromosome 1 (PϽ0.001). We also observed in epithelial cells of renal cysts a preponderance of monosomy for chromosome 16 over monosomy for chromosome 1 (PϽ0.024).

show abstract

Alcoholic Carbowax Prefixation and Formal Alcohol Fixation

Cited by 5 publications

References 18 publications

Factors predicting successful DNA recovery from archival cervical smear samples

Factors predicting successful DNA recovery from archival cervical smear samples

Optimal Prefixation of Cells To Demonstrate Apoptosis by the TUNEL Method

Loss of Chromosome 16 From Renal Epithelial Cells in Humans

Contact Info

Product

Resources

About