Albumin: Source, preparation, determination, applications, and prospects

Hutapea, Tri Paus Hasiholan; Madurani, Kartika A.; Syahputra, Muhammad Yudha; Hudha, Moh. Nuril; Asriana, Andi Nina; Suprapto, Suprapto; Kurniawan, Fredy

doi:10.1016/j.jsamd.2023.100549

Cited by 7 publications

(5 citation statements)

References 179 publications

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“…At pH values

3.5, electrostatic interactions can occur. The isoelectric point of albumin, which is the pH value at which it has no electrical charge, is found between 4.6 and 4.9 [ 63 ]. At lower pH values, the amine groups within BSA become protonated and can react with the aldehyde groups within OxG.…”

Section: Resultsmentioning

confidence: 99%

“…As mentioned before, curcumin has low bioavailability due to its water insolubility. Although it dissolves in polar organic solvents [ 63 , 64 , 65 ], the presence of organic solvents causes biopolymers to precipitate if we try to encapsulate curcumin in hydrogel films. In order to avoid this, it was decided to obtain a β-cyclodextrin–curcumin inclusion complex in a molar ratio of 2:1, which can then be included in hydrogel films based on BSA/OxG.…”

Section: Resultsmentioning

confidence: 99%

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Albumin-Based Hydrogel Films Covalently Cross-Linked with Oxidized Gellan with Encapsulated Curcumin for Biomedical Applications

Tincu (Iurciuc),

Daraba,

Jérôme

et al. 2024

Polymers

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Bovine serum albumin (BSA) hydrogels are non-immunogenic, low-cost, biocompatible, and biodegradable. In order to avoid toxic cross-linking agents, gellan was oxidized with NaIO4 to obtain new functional groups like dialdehydes for protein-based hydrogel cross-linking. The formed dialdehyde groups were highlighted with FT-IR and NMR spectroscopy. This paper aims to investigate hydrogel films for biomedical applications obtained by cross-linking BSA with oxidized gellan (OxG) containing immobilized β-cyclodextrin–curcumin inclusion complex (β-CD–Curc) The β-CD–Curc improved the bioavailability and solubility of Curc and was prepared at a molar ratio of 2:1. The film’s structure and morphology were evaluated using FT-IR spectroscopy and SEM. The swelling degree (Q%) values of hydrogel films depend on hydrophilicity and pH, with higher values at pH = 7.4. Additionally, the conversion index of -NH2 groups into Schiff bases increases with an increase in OxG amount. The polymeric matrix provides protection for Curc, is non-cytotoxic, and enhances antioxidant activity. At pH = 5.5, the skin permeability and release efficiency of encapsulated curcumin were higher than at pH = 7.4 because of the interaction of free aldehyde and carboxylic groups from hydrogels with amine groups from proteins present in the skin membrane, resulting in a better film adhesion and more efficient curcumin release.

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“…At pH values

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Albumin-Based Hydrogel Films Covalently Cross-Linked with Oxidized Gellan with Encapsulated Curcumin for Biomedical Applications

Tincu (Iurciuc),

Daraba,

Jérôme

et al. 2024

Polymers

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“…Different water solubility has been found for proteins. Crambin has been reported as a completely water-insoluble [ 21 ] , whereas the solubility more than 500 mg/mL has been determined for serum albumins [ 22 ] . Characterization of the meuNa10 has revealed that it is a water-soluble protein.…”

Section: Discussionmentioning

confidence: 99%

Identification, Characterization, and Modeling of a Bioinsecticide Protein Isolated from Scorpion Venom gland: A Three-Finger Protein

Baradaran,

Mahdavinia,

Naderi Soorki

et al. 2023

IBJ

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Background: The majority of insecticides target sodium channels. The increasing emergence of resistance to the current insecticides has persuaded researchers to search for alternative compounds. Scorpion venom gland as a reservoir of peptides or proteins, which selectively target insect sodium channels. These proteins would be an appropriate source for finding new suitable anti-insect components. Methods: Transcriptome of venom gland of scorpion M. eupeus was obtained by RNA extraction and cDNA library synthesis. The obtained transcriptome was blasted against protein databases to find insect toxins against sodium channel based on the statistically significant similarity in sequence. Physicochemical properties of the identified protein were calculated using bioinformatics software. The 3D structure of this protein was determined using homology modeling, and the final structure was assessed by MD simulation. Results: The sodium channel blocker found in the transcriptome of M. eupeus venom gland was submitted to the GenBank under the name of meuNa10, a stable hydrophilic protein consisting of 69 amino acids, with the molecular weight of 7721.77 g/mol and pI of 8.7. The tertiary structure of meuNa10 revealed a conserved CS-alpha/beta domain stabilized by eight cysteine residues. The meuNa10 is a member of the 3FP superfamily consisting of three finger-like beta strands. Conclusion: This study identified meuNa10 as a small insect sodium channel-interacting protein with some physicochemical properties, including stability and water-solubility, which make it a good candidate for further in vivo and in vitro experiments in order to develop a new bioinsecticide.

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“…Chemically, they are globular proteins comprising over 500 amino acids. There are many types of albumin, including ovalbumin, bovine serum albumin, and human serum albumin [185,186]. In humans, albumin, particularly human serum albumin, plays a key role in maintaining oncotic pressure and transporting substances such as hormones, fatty acids, and drugs.…”

Section: Albumin-based Drug Carriersmentioning

confidence: 99%

Advanced Drug Carriers: A Review of Selected Protein, Polysaccharide, and Lipid Drug Delivery Platforms

Jamroży,

Kudłacik-Kramarczyk,

Drabczyk

et al. 2024

IJMS

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Studies on bionanocomposite drug carriers are a key area in the field of active substance delivery, introducing innovative approaches to improve drug therapy. Such drug carriers play a crucial role in enhancing the bioavailability of active substances, affecting therapy efficiency and precision. The targeted delivery of drugs to the targeted sites of action and minimization of toxicity to the body is becoming possible through the use of these advanced carriers. Recent research has focused on bionanocomposite structures based on biopolymers, including lipids, polysaccharides, and proteins. This review paper is focused on the description of lipid-containing nanocomposite carriers (including liposomes, lipid emulsions, lipid nanoparticles, solid lipid nanoparticles, and nanostructured lipid carriers), polysaccharide-containing nanocomposite carriers (including alginate and cellulose), and protein-containing nanocomposite carriers (e.g., gelatin and albumin). It was demonstrated in many investigations that such carriers show the ability to load therapeutic substances efficiently and precisely control drug release. They also demonstrated desirable biocompatibility, which is a promising sign for their potential application in drug therapy. The development of bionanocomposite drug carriers indicates a novel approach to improving drug delivery processes, which has the potential to contribute to significant advances in the field of pharmacology, improving therapeutic efficacy while minimizing side effects.

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Albumin: Source, preparation, determination, applications, and prospects

Cited by 7 publications

References 179 publications

Albumin-Based Hydrogel Films Covalently Cross-Linked with Oxidized Gellan with Encapsulated Curcumin for Biomedical Applications

Albumin-Based Hydrogel Films Covalently Cross-Linked with Oxidized Gellan with Encapsulated Curcumin for Biomedical Applications

Identification, Characterization, and Modeling of a Bioinsecticide Protein Isolated from Scorpion Venom gland: A Three-Finger Protein

Advanced Drug Carriers: A Review of Selected Protein, Polysaccharide, and Lipid Drug Delivery Platforms

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