Albumin precursor and Hsp70 modulate corneal wound healing in an organ culture model

Mushtaq, Shamim; Naqvi, Zoon; Siddiqui, Anwar Ali; Ahmed, Nikhat

doi:10.1016/j.acthis.2009.07.009

Cited by 16 publications

(21 citation statements)

References 20 publications

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“…These data indicate that Runx3 negatively regulates Smad3 directly or indirectly during lung wound healing. The increased protein expression of HSP70 detected during the active phase of cell migration in the corneal epithelium suggests its possible role in wound healing (Mushtaq et al 2007(Mushtaq et al , 2009). Moreover, HSP70 plays an important role in gastric wound healing (Odashima et al 2007).…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have reported that various signaling molecules such as pErk, transforming growth factorbeta 1 (Tgf-β1), Clara cell secretory protein (CCSP), pJnk, Smad3, and heat shock protein 70 (HSP70) are closely related to wound healing (Ashcroft et al 1999;Rämet et al 2002;Reynolds et al 2005;Mushtaq et al 2007Mushtaq et al , 2009Teranishi et al 2009;Xing et al 2010;Zhou et al 1996). Phosphorylated ERK1/2 was detected in human corneal epithelial cells at the wound margin during wound healing (Teranishi et al 2009).…”

Section: Introductionmentioning

confidence: 96%

“…Mice lacking Smad3 show accelerated wound healing (Ashcroft et al 1999). In addition, HSP70 plays a possible role during corneal epithelial wound repair (Mushtaq et al 2007(Mushtaq et al , 2009.…”

Section: Introductionmentioning

confidence: 99%

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Lung tissue regeneration after induced injury in Runx3 KO mice

et al. 2010

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Runx3 is essential for normal murine lung development, and Runx3 knockout (KO) mice, which die soon after birth, exhibit alveolar hyperplasia. Wound healing, tissue repair, and regeneration mechanisms are necessary in humans for proper early lung development. Previous studies have reported that various signaling molecules, such as pErk, Tgf-beta1, CCSP, pJnk, Smad3, and HSP70 are closely related to wound healing. In order to confirm the relationship between lung defects caused by the loss of function of Runx3 and wound healing, we have localized various wound-healing markers after laser irradiation in wild-type and in Runx3 KO mouse lungs at post-natal day 1. Our results indicate that pERK, Tgf-beta1, CCSP, pJnk, and HSP70 are dramatically down-regulated by loss of Runx3 during lung wound healing. However, Smad3 is up-regulated in the Runx3 KO laser-irradiated lung region. Therefore, the lung wound-healing mechanism is inhibited in the Runx3 KO mouse, which shows abnormal lung architecture, by reduced pErk, Tgf-beta1, CCSP, pJnk, and HSP70 and by induced Smad3.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Lung tissue regeneration after induced injury in Runx3 KO mice

et al. 2010

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“…They demonstrated that a fibronectin derivative phosphorylated and upregulated the amount of Hsp70 in human corneal epithelial cells and Hsp70 seemed to be lacking in wounds that heal slowly [51]. Hsp70 has also been demonstrated to act as a significant factor in wound healing of the skin [52] and in corneas of animals [53, 54] (Table 1). …”

Section: Heat Shock Proteins Involved In the Pathogenesis Of Oculamentioning

confidence: 99%

Heat Shock Proteins in the Human Eye

Urbak

Vorum

2010

International Journal of Proteomics

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Heat shock proteins (Hsps) are believed to primarily protect and maintain cell viability under stressful conditions such as those occurring during thermal and oxidative challenges chiefly by refolding and stabilizing proteins. Hsps are found throughout the various tissues of the eye where they are thought to confer protection from disease states such as cataract, glaucoma, and cancer. This minireview summarizes the placement, properties, and roles of Hsps in the eye and aims to provide a better comprehension of their function and involvement in ocular disease pathogenesis.

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“…A high level of hsp70 protein is recorded in the case of well-healing wounds, while in the case of chronic wounds its expression is low or does not occur [22]. Both increased production of heat shock proteins and their active participation in the process of wound healing have been demonstrated over a period of approximately two to ten hours after the damage [23,24]. It is known that increased production of hsp70 protein may occur in the case of tissue damage that is caused both by internal tissue factors, such as PHSRN peptide derived from fibronectin [25], and bacterial lipopolysaccharides [26], where hsp70 expression inhibits cytokine excretion induced by lipopolysaccharides; this increases the survival rate of (for example) rats injected with lipopolysaccharides [27].…”

mentioning

confidence: 99%

The Effect of Silver Carp Skin-Derived Peptides on the Activities of VEGF and Hsp70.1 Gene Transcription Promoters

et al. 2016

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ing to replace animal collagen with another type, e.g. fish collagen [1].Studies on extracellular matrix proteins, including collagen, elastin, laminin and proteoglycans (among others), have revealed that they participate in the regulation of key cellular processes such as cell migration, adhesion and differentiation, organogenesis, mitogenesis and apoptosis [2]. This regulation is mediated by signal peptides, so-called matrikines, produced after a partial proteolysis of the matrix components; this term was first proposed by Maquart to define peptides that For over 20 years collagen of animal origin has been one of the hopes for elaborating novel therapeutic strategies. Studies conducted on animal models have demonstrated that collagen-derived peptides can be beneficial in fighting cancer, accelerating wound healing, and repairing damaged neurons, among other applications. However, all these studies were conducted using animal collagen samples, most often originating from bovine or porcine skin. Currently, due to reported cases of bovine spongiform encephalopathy (BSE) and foot-and-mouth disease ( AbstractBackground. Studies conducted on human cell culture models have demonstrated that collagen-derived peptides can exert a beneficial effect in medicine. However, all these studies were conducted using animal collagen samples, most often originating from bovine or porcine skin. Currently attempts are being made to replace animal collagen with fish collagen. Objectives. The aim of the study was to compare the effect of silver carp skin-derived peptide extract on the transcriptional activities of human VEGF and hsp70.1 gene promoters inserted into the plasmids with secreted alkaline phosphatase as a reporter gene. Material and Methods. Changes in the activity of the promoters were investigated using a HEK293FT cell line transfected with pVEGF-SEAP or pHsp70-SEAP. The cells were cultured in dishes containing peptides separated using reverse-phase high performance liquid chromatography. Results. The study demonstrated that the silver carp skin-derived peptide extract exerts both an inhibitory effect on the VEGF gene promoter and activating effect on the hsp70.1 gene promoter. Higher biological activity was recorded in the case of a freshly prepared peptide extract compared to one stored at 4°C for three months. Conclusions. The silver carp skin-derived collagen peptides influence VEGF and hsp70.1 gene promoters' transcriptional activity (Adv Clin Exp Med 2016, 25, 3, 415-423).

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Albumin precursor and Hsp70 modulate corneal wound healing in an organ culture model

Cited by 16 publications

References 20 publications

Lung tissue regeneration after induced injury in Runx3 KO mice

Lung tissue regeneration after induced injury in Runx3 KO mice

Heat Shock Proteins in the Human Eye

The Effect of Silver Carp Skin-Derived Peptides on the Activities of VEGF and Hsp70.1 Gene Transcription Promoters

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