Albumin nanoparticles coated with polysorbate 80 as a novel drug carrier for the delivery of antiretroviral drug-Efavirenz

Jenita, Josephine Leno; Chocalingam, Vijaya; Wilson, Barnabas

doi:10.4103/2230-973x.138348

Cited by 31 publications

(20 citation statements)

References 21 publications

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“…DSC was performed by a diamond differential scanning calorimeter (model Diamond DSC, PerkinElmer Inc., Waltham, MA, USA). Samples were heated from 30 to 230°C at a scan speed of 5°C/min (Jenita et al 2014). The NPs' chemical structure was assessed by Fourier-transform infrared spectroscopy (FTIR, model IFS 66 s/v, Bruker, Ettlingen, Germany) using KBr discs.…”

Section: Preparation Of Cur@anps Dox@anps and Cur/ Dox@anpsmentioning

confidence: 99%

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Co-encapsulation of curcumin and doxorubicin in albumin nanoparticles blocks the adaptive treatment tolerance of cancer cells

et al. 2019

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The adaptive treatment tolerance (ATT) of cancer cells is the main encumbrance to cancer chemotherapy. A potential solution to this problem is to treat cancer cells with multiple drugs using nanoparticles (NPs).In this study, we tested the co-administration of curcumin (Cur) and doxorubicin (Dox) to MCF-7 resistant breast cancer cells to block the ATTand elicit efficient cell killing. Drugs were co-administered to cells both sequentially and simultaneously. Sequential drug co-administration was carried out by pre-treating the cells with albumin nanoparticles (ANPs) loaded with Cur (Cur@ANPs) followed by treatment with Dox-loaded ANPs (Dox@ANPs). Simultaneous drug co-administration was carried out by treating the cells with ANPs loaded with both the drugs (Cur/Dox@ANPs). We found that the simultaneous drug co-administration led to a greater intra-cellular accumulation of Dox and cell killing with respect to the sequential drug co-administration. However, the simultaneous drug co-administration led to a lower intracellular accumulation of Cur with respect to the sequential drug co-administration. We showed that this result was due to the aggregation and entrapment of Cur in the lysosomes as soon as it was released from Cur@ANPs, a phenomenon called lysosomotropism. In contrast, the simultaneous release of Dox and Cur from Cur/Dox@ANPs into the lysosomes led to lysosomal pH elevation, which, in turn, avoided Cur aggregation, led to lysosome swelling and drug release in the cytosol, and finally provoked efficient cell killing. Our study shed the light on the molecular processes driving the therapeutic effects of anti-cancer drugs co-administered to cancer cells in different manners. Biophysics ReportsRESULTS AND DISCUSSION Preparation and characterization of albumin nanoparticles loaded with Cur and/or Dox Bovine serum albumin (BSA) nanoparticles (ANPs) loaded with Cur (Cur@ANPs), Dox (Dox@ANPs), or both RESEARCH ARTICLE S. M. Motevalli et al.

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Section: Preparation Of Cur@anps Dox@anps and Cur/ Dox@anpsmentioning

confidence: 99%

“…These results validated the encapsulation of a single drug or both the drugs in the BSA matrix. Additionally, the DSC spectra of the NPs did not show the melting point peak(s) of Cur and/or Dox, which suggested that the drugs were in an amorphous state upon encapsulation in the NPs (Jenita et al 2014) (Fig. 1D).…”

Section: Introductionmentioning

confidence: 99%

Co-encapsulation of curcumin and doxorubicin in albumin nanoparticles blocks the adaptive treatment tolerance of cancer cells

et al. 2019

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“…[1][2][3][4] These research activities were stimulated by experiments which showed that nanoparticles penetrated e±ciently through the blood-brain barrier. 5 However, a commercial product for nanoparticle-assisted delivery of drugs through the cutaneous barrier is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Hair follicles as a target structure for nanoparticles

Lademann

Knorr

Richter

et al. 2015

J. Innov. Opt. Health Sci.

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For at least two decades, nanoparticles have been investigated for their capability to deliver topically applied substances through the skin barrier. Based on¯ndings that nanoparticles are highly suitable for penetrating the blood-brain barrier, their use for drug delivery through the skin has become a topic of intense research. In spite of the research e®orts by academia and industry, a commercial product permitting the nanoparticle-assisted delivery of topically applied drugs has not yet been developed. However, nanoparticles of approximately 600 nm in diameter have been shown to penetrate e±ciently into the hair follicles, where they can be stored for several days. The successful loading of nanoparticles with drugs and their triggered release inside the hair follicle may present an ideal method for localized drug delivery. Depending on the particle size, such a method would permit targeting speci¯c structures in the hair follicles such as stem cells or immune cells or blood vessels found in the vicinity of the hair follicles.

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“…A study was carried out to assess the stability of BSA nanoparticles of drug stavudine (drug-polymer ratio 1:1). The samples were stored in room temperature (15-20°C), refrigerator (3-5°C), and 37°C (Relative humidity [RH] = 75%) over a period of 3 months [16]. Samples were evaluated at 0, 1, 2, and 3 months for their drug content as well as any changes in their physical appearance.…”

Section: Stability Studiesmentioning

confidence: 99%

Development, Evaluation and Targeting of Stavudine Loaded Serum Albumin Polymer Based Nanocarriers to Hiv Reservoirs

Jenita

2019

Asian J Pharm Clin Res

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Objective: Stavudine is an antiretroviral drug that is part of the nucleoside reverse transcriptase inhibitor (NRTIs) family, which is used to delay the progression of HIV infection. The present investigation involves the development and evaluates stavudine loaded nanocarriers using natural polymer bovine serum albumin (BSA). Methods: The desolvation technique was used to prepare nanoparticles and coated with 1% v/v polysorbate 80 to improve the targeting of drugs to the organs (HIV reservoirs). Biodistribution studies were also investigated for the best formulation to determine the targeting efficiency of nanocarriers loaded stavudine and compared with pure compound. Results: The formulated nanocarriers have shown mean particle size below 300 nm, zeta potential in the range of -16.5 Mv, encapsulation efficiency in the range of 50.10 to 73.7%, drug loading in the range of 14.73 to73.84%. Cumulative % drug release was in the range of 24.72 to 71.20% and release kinetics studies showed that the mechanism of drug release was controlled simultaneously by diffusion and erosion of the matrix type formulations. The stability studies over a period of three months confirmed the stability of BSA nanoparticles. Biodistribution studies demonstrated that nanoparticles coated with 1% v/v polysorbate 80 were able to reach the HIV reservoirs in an amount higher than that of uncoated stavudine nanoparticles or pure drug itself. Conclusion: The method adopted is simple and the biodistribution studies demonstrated that nanoparticles coated with 1% polysorbate 80 were able to reach the selected organs in an amount higher than that of uncoated stavudine nanoparticles or pure drug itself.

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Albumin nanoparticles coated with polysorbate 80 as a novel drug carrier for the delivery of antiretroviral drug-Efavirenz

Cited by 31 publications

References 21 publications

Co-encapsulation of curcumin and doxorubicin in albumin nanoparticles blocks the adaptive treatment tolerance of cancer cells

Co-encapsulation of curcumin and doxorubicin in albumin nanoparticles blocks the adaptive treatment tolerance of cancer cells

Hair follicles as a target structure for nanoparticles

Development, Evaluation and Targeting of Stavudine Loaded Serum Albumin Polymer Based Nanocarriers to Hiv Reservoirs

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