Albumin nano-encapsulation of caffeic acid phenethyl ester and piceatannol potentiated its ability to modulate HIF and NF-kB pathways and improves therapeutic outcome in experimental colitis

Tambuwala, Murtaza M.; Khan, Mohammed Naeem; Thompson, Paul D.; McCarron, Paul A.

doi:10.1007/s13346-018-00597-9

Cited by 43 publications

(26 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This difference is useful in its spectrofluorometric analysis because this amino acid is the main residue responsible for the protein's intrinsic fluorescence [37][38][39]. Due to its biocompatibility, biodegradable, non-toxic, and non-immunogenic features, it has been proposed that BSA can offer a suitable nanocarrier system for drug delivery [40][41][42][43].…”

Section: Introductionmentioning

confidence: 99%

Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α

Aljabali

Bakshi

Hakkim

et al. 2020

Cancers

Self Cite

View full text Add to dashboard Cite

Piceatannol (PIC) is known to have anticancer activity, which has been attributed to its ability to block the proliferation of cancer cells via suppression of the NF-kB signaling pathway. However, its effect on hypoxia-inducible factor (HIF) is not well known in cancer. In this study, PIC was loaded into bovine serum albumin (BSA) by desolvation method as PIC–BSA nanoparticles (NPs). These PIC–BSA nanoparticles were assessed for in vitro cytotoxicity, migration, invasion, and colony formation studies and levels of p65 and HIF-1α. Our results indicate that PIC–BSA NPs were more effective in downregulating the expression of nuclear p65 and HIF-1α in colon cancer cells as compared to free PIC. We also observed a significant reduction in inflammation induced by chemical colitis in mice by PIC–BSA NPs. Furthermore, a significant reduction in tumor size and number of colon tumors was also observed in the murine model of colitis-associated colorectal cancer, when treated with PIC–BSA NPs as compared to free PIC. The overall results indicate that PIC, when formulated as PIC–BSA NPs, enhances its therapeutic potential. Our work could prompt further research in using natural anticancer agents as nanoparticels with possible human clinical trails. This could lead to the development of a new line of safe and effective therapeutics for cancer patients.

show abstract

Section: Introductionmentioning

confidence: 99%

Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α

Aljabali

Bakshi

Hakkim

et al. 2020

Cancers

Self Cite

View full text Add to dashboard Cite

show abstract

“…Khan et al reported that CAPE can suppress inflammation-induced MPO activity and proinflammatory cytokine production and can enhance epithelial barrier function in experimental colitis (10). Some recent studies also demonstrated that CAPE has a protective effect on colitis (33,34). However, it remains unknown whether CAPE can inhibit CAC and what the underlying mechanism is.…”

Section: Discussionmentioning

confidence: 99%

Caffeic Acid Phenethyl Ester Prevents Colitis-Associated Cancer by Inhibiting NLRP3 Inflammasome

et al. 2020

View full text Add to dashboard Cite

Long-lasting inflammation in the intestinal tract renders individuals susceptible to colitisassociated cancer (CAC). The NOD-like receptor protein 3 (NLRP3) inflammasome plays a key role in the progression of inflammatory bowel disease and CAC. Therefore, identifying effective drugs that prevent CAC by targeting NLRP3 inflammasome is of great interest. Here, we aimed to evaluate the anti-inflammatory effect of caffeic acid phenethyl ester (CAPE) on bone marrow-derived macrophages (BMDMs), THP-1 cells, and azoxymethane/dextran sulfate sodium (AOM/DSS)-induced colon cancer mouse model. We also investigated the anti-tumor mechanism of CAPE. We found that CAPE decreased NLRP3 inflammasome activation in BMDMs and THP-1 cells and protected mice from colorectal cancer induced by AOM/DSS. CAPE regulated NLRP3 at the posttranscriptional level by inhibiting reactive oxygen species (ROS) production. However, CAPE did not affect NLRP3 or IL-1β transcription, but instead enhanced NLRP3 binding to ubiquitin molecules, promoting NLRP3 ubiquitination, and contributing to the antitumor effect in the AOM/DSS mouse model. Moreover, CAPE suppressed the interaction between NLRP3 and CSN5 but enhanced that between NLRP3 and Cullin1 both in vivo and in vitro. Altogether, our findings demonstrate that CAPE prevents CAC by post-transcriptionally inhibiting NLRP3 inflammasome. Thus, CAPE may be an effective candidate for reducing the risk of CAC in patients with inflammatory bowel disease.

show abstract

“…However, the main limitations of CAPE are associated with its low water solubility and instability in physiological media. The incorporation of CAPE in appropriate drug delivery systems could overcome these limitations . For instance, Wadhwa et al .…”

Section: Introductionmentioning

confidence: 99%

“…The incorporation of CAPE in appropriate drug delivery systems could overcome these limitations. 29,30 For instance, Wadhwa et al 31 reported that the complexation of CAPE with gamma cyclodextrin might avoid its degradation in culture medium and lead to a higher anti-tumor activity.…”

Section: Introductionmentioning

confidence: 99%

Functional amphiphilic block copolyethers as carriers of caffeic acid phenethyl ester

Valchanova

Yordanov

Tzankova

et al. 2019

Polymer International

View full text Add to dashboard Cite

Amphiphilic diblock copolymers consisting of hydrophilic polyglycidol (PG) and hydrophobic poly(allylglycidyl ether) (PAGE) were prepared by sequential anionic ring‐opening polymerization of allylglycidyl ether and ethoxyethyl glycidyl ether followed by removal of the protective ethoxyethyl groups. The polymerization was initiated by partially deprotonated dodecanol and performed in solvent‐free conditions. The copolymers were composed of a hydrophobic dodecyl residue attached to a block of PAGE with a fixed degree of polymerization (dp = 44) and differing in length of the PG block (dp = 16 and 66, corresponding to PG contents of 25 and 60 mol%, respectively). The two copolymers were spontaneously soluble in water. Above a certain critical concentration, they formed well‐defined self‐assembled nanoparticles. Their characterization parameters were determined by static and dynamic light scattering. The aggregates of the more hydrophobic copolymer, C12‐PAGE‐PG25, were characterized by considerably larger dimensions and molar mass, reaching 78.6 nm and 253.0 × 106 g mol−1, respectively, than those of the more hydrophilic copolymer, C12‐PAGE‐PG60. The hydrophobic moieties were proved to create a favorable environment for solubilization of caffeic acid phenethyl ester (CAPE) (the main active ingredient of propolis with cytotoxic and antioxidant activities), whereas the numerous hydroxyl groups from the PG moieties brought additional benefits related to the biocompatibility of the copolymers. Preliminary experiments with L929 fibroblast cells showed that the aggregates displayed no signs of toxicity in the applied in vitro test system, suggesting their appropriateness as a drug delivery platform. The CAPE‐loaded aggregates, however, showed dose‐dependent cytotoxic effects, indicating that CAPE retained its cytotoxic activity. © 2019 Society of Chemical Industry

show abstract

Albumin nano-encapsulation of caffeic acid phenethyl ester and piceatannol potentiated its ability to modulate HIF and NF-kB pathways and improves therapeutic outcome in experimental colitis

Cited by 43 publications

References 56 publications

Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α

Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α

Caffeic Acid Phenethyl Ester Prevents Colitis-Associated Cancer by Inhibiting NLRP3 Inflammasome

Functional amphiphilic block copolyethers as carriers of caffeic acid phenethyl ester

Contact Info

Product

Resources

About